Measurement of anti‐thyroglobulin IgG in urine of patients with autoimmune thyroid diseases by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay)

Yogi, Yoshikuni; Hirota, Kouichi; Kohno, Takeyuki; Toshimori, Hirotaka; Matsukura, Shigeru; Setoguchi, Toshiaki; Ishikawa, Eiji

doi:10.1002/jcla.1860070112

Cited by 7 publications

(11 citation statements)

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“…10,15,16 In contrast, IgG autoantibodies can be found in the urine of patients with autoimmune thyroid disease, 17,18 pernicious anaemia, 19 and interstitial cystitis. 20 In addition, there is ample evidence demonstrating that IgG-class HIV-1 antibodies can be detected in the urine of HIV-1 seropositive and even seronegative patients.…”

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confidence: 99%

Mucosal Immunity and Primary Biliary Cirrhosis: Presence of Antimitochondrial Antibodies in Urine

et al. 2000

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We have shown that IgA-class antimitochondrial autoantibodies (AMA) can be detected in the bile and saliva of patients with PBC, suggesting that AMA are secreted into the luminal fluid across bile ducts and salivary glands. These data prompted us to determine whether AMA of the IgA isotype may be transported across other epithelial mucosa. Therefore, we tested for the presence of AMA in the urine specimens of 83 patients with PBC and 58 non-PBC controls including healthy individuals and patients with other liver diseases. Patients enrolled in this study had no history of renal disease, and we confirmed there was less than 50 g/mL of protein in each of the urine specimens. Interestingly, we found that AMA were present in the urine of 71/83 (86%) of all patients with PBC and in 71/78 (91%) of patients with PBC that were serum AMA positive. In contrast, AMA were not detected in any of the 58 control urine specimens. Of particular interest, AMA of the IgA isotype was present in 57/83 (69%) of patients with PBC, and in 52 of these 57, we found secretory-type IgA. In a nested random subgroup of urine samples, the prevalence of the IgA2 AMA was 6/18 (33%), significantly lower than in matched serum samples, 13/16 (81%, P ‫؍‬ .007). These data show that AMA of the IgA isotype is secreted into urine from the uroepithelium of patients with PBC, and support the thesis that PBC originated from either a mucosal challenge or a loss of mucosal tolerance. (HEPATOLOGY 2000;32: 910-915.)

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Mucosal Immunity and Primary Biliary Cirrhosis: Presence of Antimitochondrial Antibodies in Urine

et al. 2000

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“…Those levels were well correlated to serum levels, whether the values were corrected by the concentration of creatinine or IgG in urine or IgG in both urine and serum (Fig. 25) (14). This indicated that antithyroglobulin IgCi in urine derived from that in blood.…”

Section: -D-galactosidase From E Coli As Label (Open Symbols) and mentioning

confidence: 73%

“…The presence of antithyroglobulin IgG at low concentrations in healthy subjects was confirmed by preincubation of sera with excess of thyroglobulin in the presence of excess of inactive P-D-galactosidase ( Fig. 15) (12,14).…”

Section: Application Of Immune Complex Transfer Enzyme Immunoassay Fomentioning

confidence: 85%

“…coli as label, antithyroglobulin IgC was measurable in urine of most of patients with Graves' disease and chronic thyroiditis (Fig. 24) (14). Those levels were well correlated to serum levels, whether the values were corrected by the concentration of creatinine or IgG in urine or IgG in both urine and serum (Fig.…”

Section: -D-galactosidase From E Coli As Label (Open Symbols) and mentioning

confidence: 90%

“…26) and, therefore, only high levels of antithyroglobulin TgG in urine were correlated to those in serum ( Fig. 27) (14).…”

Section: -D-galactosidase From E Coli As Label (Open Symbols) and mentioning

confidence: 94%

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Principle and applications of ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for antibodies in body fluids

Ishikawa¹,

Hashida²,

Kohno³

et al. 1993

Clinical Laboratory Analysis

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The sensitivity and specificity of enzyme immunoassay for antibodies in body fluids have been improved considerably by transferring the complex of labelled antigen and antibody to be detected from one solid phase to another to eliminate interfering substance(s) in the samples (immune complex transfer enzyme immunoassay). Usefulness of the new method has been tested for antibodies in serum as well as in urine. Anti-thyroglobulin IgG could be measured not only in serum of all patients with autoimmune thyroid diseases and almost all healthy subjects but also in the urine of most of the patients. Anti-HTLV-I IgG was unequivocally demonstrated in some of sera, which were indeterminate or negative by Western blotting, and diagnosis of HIV infection by detecting anti-HIV IgG in urine and saliva would be possible with higher reliability than by conventional methods.

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Improved measurement of anti‐thyroglobulin IgG in urine of patients with autoimmune thyroid diseases by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay)

Yogi

Hirota

Hashida

et al. 1993

Clinical Laboratory Analysis

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Previously, antithyroglobulin IgG was assayed in dialyzed urine from patients with autoimmune thyroid diseases by a sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay), and most of the assay results were useful as a diagnostic aid for autoimmune thyroid diseases. However, dialysis of urine was laborious and time-consuming, and some results were less reliable due to low levels of anti-thyroglobulin IgG in urine. This paper describes some improvements of the assay. Useful assay results could be obtained for most of urine samples without dialysis, although some interfering substance(s) was suggested to be present in some urine samples before dialysis. Accurate assay results with no interference could be obtained after gel filtration by only two min centrifugation in place of dialysis. More reliable assay results for urine samples containing low levels of antithyroglobulin IgG were obtained after concentration using a molecular sieve.

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Measurement of anti‐thyroglobulin IgG in urine of patients with autoimmune thyroid diseases by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay)

Cited by 7 publications

References 11 publications

Mucosal Immunity and Primary Biliary Cirrhosis: Presence of Antimitochondrial Antibodies in Urine

Mucosal Immunity and Primary Biliary Cirrhosis: Presence of Antimitochondrial Antibodies in Urine

Principle and applications of ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for antibodies in body fluids

Improved measurement of anti‐thyroglobulin IgG in urine of patients with autoimmune thyroid diseases by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay)

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