Measurement of carvedilol in plasma by high-performance liquid chromatography with electrochemical detection

Machida, Maiko; Watanabe, Masato; Takechi, Shigeru; Kakinoki, Shigeo; Nomura, Akio

doi:10.1016/j.jchromb.2003.09.039

Cited by 40 publications

(25 citation statements)

References 18 publications

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“…Carvedilol has been determined in biological fluids using highperformance liquid chromatography (HPLC) with fluorescence 1-7 , electrochemical detection 8 or mass spectrometry (MS) MS detection 13 . Reverse-phase (RP)-HPLC methods were described for the analysis of Carvedilol racemate 1, 3-5, 8, 9 or carvedilol enantiomers using chiral derivatization 2,10 .…”

Section: Introductionmentioning

confidence: 99%

“…HPLC methods using a chiral stationary phase have been reported for the analysis of carvedilol enantiomers 6,7 . The clean-up procedures for the extraction of carvedilol from biological matrix consist of protein precipitation 5,6,10,12 , solid-phase extraction (SPE) 2,3,9 , liquid-liquid extraction (LLE) 6,7,[11][12][13] , combinations of protein precipitation with SPE 4 or combinations of LLE with back-extraction 1,8 . Those methods use a large amount of biological samples (0.15-1.0 mL plasma or 2-5 mL urine samples) in order to obtain the high sensitivity or include time-consuming extraction procedures and/or relatively long run time.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Carvedilol and Its Metabolite in Human Plasma Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Janjanam¹,

Bimireddy²

2017

IJPTR

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: A rapid, sensitive and selective method for the determination of Carvedilol and its metabolite in human plasma was developed using liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). Carvedilol, its metabolite 4-OH carvedilol and abacavir (internal standard) were extracted from human plasma by solid phase extraction technique (SPE) and analyzed on an Discovery column (C8, 50 × 4.6 mm, 5µ) with the mobile phase of acetonitrile -0.1% formic acid in water (70:30 v/v). The analytes were detected using an electro spray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The standard curve was linear (r = 0.9998) over the concentration range of 0.5-100 ng/mL and 0.3-40 ng/mL for carvedilol and 4-OH carvedilol respectively. The lower limit of quantification for carvedilol was 0.5 ng/mL and 0.3 ng/mL for carvedilol and 4-OH carvedilol respectively using 500 µL plasma samples. The coefficient of variation and relative error for intra-and inter-assay at four QC levels were 3.37 to 9.53 %, 4.76 to 7.01% and 3.31 to 6.91%, 5.23 to 6.64 % respectively. The matrix effect for carvedilol, 4-OH carvedilol and abacavir were practically absent. The extraction recoveries of carvedilol, 4-OH carvedilol and abacavir were 78.90, 83.25 and 85.20%, respectively.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Carvedilol and Its Metabolite in Human Plasma Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Janjanam¹,

Bimireddy²

2017

IJPTR

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“…β-blockers affect the heart and blood circulation (Flanagan, 1998). Analytical methods such as HPLC (Belal et al, 2013;Machida et al, 2003;Zarghi et al, 2007), differential pulse voltammetry (Dogan and Ozkan, 2005) and spectrofuorimetry (Önal, 2011;Cardoso et al, 2007) have been used for the determination of CRV. Fig.…”

Section: Introductionmentioning

confidence: 99%

Application of Bromocresol Green and Bromothymol Blue for the Extractive Spectrophotometric Determination of Anti-hypertensive Drugs

El‐Didamony¹,

Hafeez²,

Saad³

2015

J App Pharm Sci

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Simple, selective and highly sensitive spectrophotometric methods are proposed for the rapid and accurate determination of anti-hypertensive drugs namely telmisartan (TEL), propranolol (PRO), bisoprolol (BIS) and carvedilol (CRV) in tablets and biological fluids using bromocressol green (BCG) and bromothymol blue (BTB). The developed methods involve formation of stable yellow colored dichloromethane extractable ion-pair complexes of the amino derivative of four antihypertensive drugs such as TEL, PRO, BIS and CRV with two sulphonphthalein acid dyes, namely; BCG and BTB in acidic buffer. The effect of optimum conditions via pH on the ion-pair formation, reagent concentration, time and temperature and solvent was studied. The composition of the ion-pairs was found 1: 1 by Job's method. The established methods having high sensitivity and good selectivity could be applied to the determination of the studied drugs in pharmaceutical, urine and blood serum samples with satisfactory results. The results obtained are good agreement with experimental data. The reaction mechanism was also discussed.

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“…Carvedilol has been quantified in biological fluids using high-performance liquid chromatography (HPLC) with fluorescence [1][2][6][7][8][9][10][11] or electrochemical detector [12], mass spectrometry [13][14][15], hydrophilic interaction liquid chromatography (HILIC) with tandem mass spectrometry [16], capillary electrophoresis-ultra violet detector [17] and gas chromatography (GC)-MS detector [18]. The clean-up procedures for the extraction of carvedilol from biological matrix has been carried out by protein precipitation [8][9]13,15], solid-phase extraction (SPE) [2,6,12], liquid-liquid extraction (LLE) [10,14,18], combinations of protein precipitation with SPE [7] or combinations of LLE with back-extraction [1,11].…”

Section: Introductionmentioning

confidence: 99%

“…The clean-up procedures for the extraction of carvedilol from biological matrix has been carried out by protein precipitation [8][9]13,15], solid-phase extraction (SPE) [2,6,12], liquid-liquid extraction (LLE) [10,14,18], combinations of protein precipitation with SPE [7] or combinations of LLE with back-extraction [1,11]. However, these methods used a large volume of biological fluids (0.15-1.0 ml plasma or 2-5 ml urine), time-consuming pre-treat procedures for LLE, SPE or derivatization steps and required a long run-time (20 min) to obtain high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Carvedilol Assay in Human Plasma Using a High-Performance Liquid Chromatography with Mass/Mass Spectrometer Detection Employed for a Bioequivalence Study

Kim¹,

Lee²

2010

AJAC

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A method for the determination of carvedilol in human plasma was developed using a high-performance liquid chromatography with tandem mass spectrometer (HPLC-MS/MS). Plasma samples were deproteinized using acetonitrile and the supernatant was directly injected onto the HPLC column without any preparative steps. Chromatography was performed on a reversed-phase (C 18 ) column with isocratic mobile phase for 2 min. The calibration curve was linear over the range of 2 to100 ng/ml (R 2 > 0.9998) and the lower limit of quantitation (LLOQ) was 2 ng/ml. This method showed acceptable precision and accuracy, good recovery from the plasma matrix, and stability during the analytical procedures. When its application to the bioequivalence test of two carvedilol 25 mg tablet formulations in male healthy 28 volunteers, this validated analysis method was appropriate resulting in the bioequivalence of two formulations: no statistically significant difference was observed between the logarithmic transformed area under curve (AUC) and maximum plasma concentration (C max ) values of the two formulations. The 90% confidence interval for the ratio of the above mentioned two parameters were within the bioequivalence limit of 0.80-1.25. These results suggested that the HPLC-MS/MS analysis method developed was suitable for the carvedilol analysis in human plasma.

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Measurement of carvedilol in plasma by high-performance liquid chromatography with electrochemical detection

Cited by 40 publications

References 18 publications

Analysis of Carvedilol and Its Metabolite in Human Plasma Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Analysis of Carvedilol and Its Metabolite in Human Plasma Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Application of Bromocresol Green and Bromothymol Blue for the Extractive Spectrophotometric Determination of Anti-hypertensive Drugs

Rapid and Sensitive Carvedilol Assay in Human Plasma Using a High-Performance Liquid Chromatography with Mass/Mass Spectrometer Detection Employed for a Bioequivalence Study

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