Measurement of competitive adsorption isotherms by frontal chromatography

Jacobson, Jana; Frenz, John; Horváth, Csaba

doi:10.1021/ie00061a009

Cited by 119 publications

(46 citation statements)

References 9 publications

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“…(17), continuum fractioning and tandem HPLC analysis for the determination of intermediate plateau concentrations is necessary [7,8]. Additionally, to determine the full competitive adsorption isotherm, multiple FA sequences have to be carried out with mixtures of two components in different relative ratios.…”

Section: Famentioning

confidence: 99%

Determination of adsorption isotherms by means of HPLC: Adsorption mechanism elucidation and separation optimization

Marchetti¹,

Cavazzini²,

Pasti³

et al. 2009

J of Separation Science

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The purpose of this review is to illustrate the most important techniques for isotherm determination by means of HPLC. Starting on the traditional Frontal Analysis approach, Frontal Analysis by Characteristic Point, Elution by Characteristic Point, Perturbation Method in its different applications will be considered to conclude with the most recent Inverse Method approach. Since many of these techniques are based on the fundamentals of nonlinear chromatography, a short overview of the theory of nonlinear chromatography is presented. Emphasis is given to the most recent applications of these techniques for pharmaceutical applications, characterization of binding mechanisms, bioaffinity studies, molecular and chiral recognition processes.

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Section: Famentioning

confidence: 99%

Determination of adsorption isotherms by means of HPLC: Adsorption mechanism elucidation and separation optimization

Marchetti¹,

Cavazzini²,

Pasti³

et al. 2009

J of Separation Science

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“…The FA method was adapted to measure competitive isotherms by Jacobson et al (8). However, this approach is tedious and time-consuming, even for the binary case.…”

Section: T Th He Er Rm Mo Od Dy Yn Na Am MI Ic Cs Smentioning

confidence: 99%

“…It is also necessary, however, to collect and analyze eluent fractions on the intermediate plateaus of the breakthrough curves (1,8).…”

Section: T Th He Er Rm Mo Od Dy Yn Na Am MI Ic Cs Smentioning

confidence: 99%

Peer Reviewed: Nonlinear Effects in LC and Chiral LC

Fornstedt¹,

Guiochon²

2001

Anal. Chem.

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See https://pubs.acs.org/sharingguidelines for options on how to legitimately share published articles. N O V E M B E R 1 , 2 0 0 1 / A N A LY T I C A L C H E M I S T R Y6 0 9 A o achieve high resolution and accurate peak integration, analytical chromatographers choose experimental conditions under which nonlinear effects are minimized. Careful use of these same effects allows them to design profitable separations.Nonlinear effects are ubiquitous. There are almost no experimental conditions under which they do not exist, although under many they are negligible. The key to understanding nonlinear effects lies in the equilibrium isotherms, the relationship between the solute concentrations at equilibrium in the two phases of the system. In most separations, particularly in chiral ones, the determination of these isotherms also allows more accurate investigation of the retention mechanism. We will show how band profiles can be modeled, why the model results are useful, and how systematic isotherm mea surements allow a deeper understanding of certain retention mechanisms.W Wh ha at t i is s n no on nl li in ne ea ar r c ch hr ro om ma at to og gr ra ap ph hy y? ?The goal of preparative chromatography is the rapid preparation of a significant amount of one component in a mixture to a sufficient degree of purity (1). Thus, large amounts of high-concentration samples are injected. Because of the finite surface area of the stationary phase in the column, the relative amount of a component adsorbed at equilibrium usually decreases as concentration increases, affecting retention. This phenomenon characterizes nonlinear chromatography.Peaks tail strongly in preparative chromatography, in contrast to the symmetrical peaks in analytical (i.e., linear) chromatography (2). Most often, nonlinear tailing peaks have sharp fronts and long, diffuse rear boundaries. The retention time of the high-concentration front decreases with increasing sample size, while the end of the peak tail has a con-

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“…It has been extended to binary mixture by Jacobson et al (1987). As for single-component isotherms, successive frontal steps of solutions of increasing concentration, but constant relative composition, are introduced, and the composition of the intermediate plateau formed is determined by on-line chromatographic analysis.…”

Section: Introductionmentioning

confidence: 99%

“…The advantages of the method over the classical frontal analysis with binary mixtures (Jacobson et al, 1987) is the improved accuracy, the shorter time required to acquire the experimental data, and the much smaller amounts of chemicals needed. The main inconvenience is that the isotherm points are not obtained directly, but rather the experimental data must be fitted on an isotherm.…”

Section: Introductionmentioning

confidence: 99%

Prediction of binary, overloaded elution profiles using the simple wave effect

Katti

Guiochon

1990

AIChE Journal

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The separation of binary mixtures in overloaded elution chromatography has been investigated experimentally and theoretically using the Rouchon-Golshan-Shirazi implementation of the semiideal model (equilibrium-dispersive model). A competitive Langmuir isotherm with parameters determined from single-component data gives good prediction of the experimental data. Improved predictions, however, can be obtained by directly measuring the binary isotherm using the method of the hodograph transform.

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Measurement of competitive adsorption isotherms by frontal chromatography

Cited by 119 publications

References 9 publications

Determination of adsorption isotherms by means of HPLC: Adsorption mechanism elucidation and separation optimization

Determination of adsorption isotherms by means of HPLC: Adsorption mechanism elucidation and separation optimization

Peer Reviewed: Nonlinear Effects in LC and Chiral LC

Prediction of binary, overloaded elution profiles using the simple wave effect

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