Measurement of Frequency of Ciliary Beats of Human Respiratory Epithelium

Yager, Jean; Chen, Tzeng-Ming; Dulfano, Mauricio J.

doi:10.1378/chest.73.5.627

Cited by 122 publications

(36 citation statements)

References 12 publications

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“…Historically, high-speed cinematography, where light-detected images were played back at slow speeds in order to allow direct frequency and beat pattern measurements, was regarded as the optimal method for ciliary beat analysis [30]. Because of cost, difficult image processing, and lack of real time analysis, this method was later replaced by video-coupled photomultiplier and photodiode techniques, which estimate the beat frequency indirectly by detecting changes in light intensity passing through the field of beating cilia [31,32]. Recent advances and increased availability of digital high-speed video technology has allowed capturing images of beating ciliated cells at a rate of 250 frames/s [33,34 ,35 ,36-41].…”

Section: Ex Vivo/in Vitro Cilia Function and Methods Of Assessmentmentioning

confidence: 99%

Mucociliary clearance – a critical upper airway host defense mechanism and methods of assessment

Antunes

Cohen²

2007

Current Opinion in Allergy &Amp; Clinical Immunology

151

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Section: Ex Vivo/in Vitro Cilia Function and Methods Of Assessmentmentioning

confidence: 99%

Mucociliary clearance – a critical upper airway host defense mechanism and methods of assessment

Antunes

Cohen²

2007

Current Opinion in Allergy &Amp; Clinical Immunology

151

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“…These electrical signals were ampli fied, displayed on the oscilloscopic screen (Leader, , and recorded by a pen recorder (Pana sonic, VP62I3). Measurements of CBF were aver aged from clumps of two or more cells with free bor ders devoid of debris [Yager et al, 1978], In addition to CBF, ciliary coordination was assessed by the im age of beating recorded on a video camera (Hama matsu Photonics, C-1846-01) with a 0.75-inch (1.9-cm) video cassette recorder (Sony, VO-5800) capable of freeze-frame replay.…”

Section: Methodsmentioning

confidence: 99%

Stimulation of Ciliary Activity by Indomethacin in Rabbit Tracheal Epithelial Organ Culture

Tamaoki¹,

Kondo²,

Takizawa³

1988

Respiration

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Inflammatory mediators influence mucociliary transport function in the lung, which may be dependent on the beating of the underlying cilia. To determine whether a nonsteroidal anti-inflammatory drug affects this function, and, if so, what is the mechanism action, we examined the effect of indomethacin on ciliary beat frequency (CBF) of cultured rabbit tracheal epithelial cells by a photoelectric technique. Addition of indomethacin dose-dependently enhanced CBF without any ciliary disco-ordination, the maximal increase and K_m being 26.5 ± 4.8% (mean ± SE, p < 0.001) and 6.8 × 10^-7M, respectively. Pretreatment of tissues with nordihydroguaretic acid (10^-5M), an inhibitor of the lipoxygenase pathway, did not change the baseline CBF, but inhibited the response of CBF to 3 × 10^-6M indomethacin (p < 0.01). These results suggest that indomethacin may accelerate mucociliary clearance through the augmentation of ciliary motility and that this ciliostimulation may be mediated by leukotrienes produced by activation of the lipoxygenase pathway of arachidonic acid metabolism

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“…Ciliary beat frequency (CBF) measurements were carried out with a photometric technique [11,12]. The same ciliated area of each sample was observed throughout the experimental period, and CBF measurements were taken at baseline (B) and 1 (T|), 15 (T,), and 30 (T3) min after perfusion with the drug and after washing the chamber with culture medium only (T,) at the end of each concen tration run.…”

Section: Methodsmentioning

confidence: 99%

Effect of Amiloride on Human Bronchial Ciliary Activity in vitro

Benedetto

Lopez-Vidriero²,

Carratù³

et al. 1990

Respiration

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Amiloride (AM) reduces mucociliary transport in the frog palate in vitro preparation, whereas in vivo inhalation of AM enhances mucociliary and cough clearance in cystic fibrosis patients. AM influences the bioelectric properties of the respiratory mucosa, and since electrical activity and ciliary activity are strictly related in the beating cell, an effect on ciliary function cannot be excluded. We have, therefore, investigated the effect of AM (10^-7 to 10^-3M) in vitro on the ciliary activity often samples of healthy human bronchial epithelium at 37 °C. The baseline ciliary beat frequency was 13.7 ± (SD)1.0 Hz. At concentrations of AM of 10^-4 and 10^-3M, small statistically significant (p < 0.01) increases in ciliary beat frequency were recorded only after 1 min of exposure to the drug, with changes of +8.2 and +7.6%, respectively. We conclude that (1) AM affects the ciliary activity of the beating cell, but (2) because of the weakness and short duration of the effect, it is unlikely that AM plays a major role on mucociliary transport

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Measurement of Frequency of Ciliary Beats of Human Respiratory Epithelium

Cited by 122 publications

References 12 publications

Mucociliary clearance – a critical upper airway host defense mechanism and methods of assessment

Mucociliary clearance – a critical upper airway host defense mechanism and methods of assessment

Stimulation of Ciliary Activity by Indomethacin in Rabbit Tracheal Epithelial Organ Culture

Effect of Amiloride on Human Bronchial Ciliary Activity in vitro

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