Measurement of mouse plasma erythropoietin by an improved ELISA

Satoh, Susumu; Nakatani, Akira; Jimaru, D.; Ueda, Masatsugu; Kohzuki, Hisaharu; Ohga, Yoshimi; Misawa, Hiromi; Takaki, Miyako

doi:10.1007/bf02585545

Search citation statements

Order By: Relevance

Paper Sections

Select...

Discussion1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2015

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We did not perform a pharmacokinetic analysis of rhEPO levels in this study. However, considering that erythropoietin is normally present at 18 mIU/mL in mice, our injection schedule of 200 IU bolus injections three times a week could lead to appreciably higher than natural endogenous levels of erythropoietin. Our objective was to inject the HEPO and EPO formulations without dilution to get a readout on their relative immunogenicity, and as a consequence we had to use the smallest reproducible injection volume; however, these small volumes still translated to very high doses in mice.…”

Section: Discussionmentioning

confidence: 99%