Measurement of MR signal and T2* in lung to characterize a tight skin mouse model of emphysema using single‐point imaging

Olsson, Lars; Lindahl, Maria; Onnervik, Per-Ola; Johansson, Lars B; Palmer, M Dean; Reimer, Martina Kvist; Hultin, Leif; Hockings, Paul D.

doi:10.1002/jmri.20840

Cited by 52 publications

(72 citation statements)

References 20 publications

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“…Indeed, proton MR imaging was successfully applied in experimental animal models of pulmonary emphysema. For example, Olsson et al (11) used single-point imaging to detect structural changes in the lungs using a tight skin experimental model. Similarly, Quintana et al (12) reported decreased MR signal intensities with a gradient-echo sequence in an experimental rat elastase-model of emphysema.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal and noninvasive assessment of emphysema evolution in a murine model using proton MRI

Żurek

Boyer

Caramelle

et al. 2011

Magnetic Resonance in Med

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Ultrashort echo time (550 ms) MR imaging was implemented to track the emphysema development in mice lung challenged with elastase. Two parameters, namely, signal intensity and T 2 *, were used to monitor the disease evolution. Nine mice were imaged before and at 24 h as well as at 3 and 8 weeks after elastase instillation. Five mice instilled with saline served as controls. At week 8, the mean normalized signal intensity 6 SD was 0.89 6 0.20 for healthy controls and 0.64 6 0.10 for animals with emphysema. Similarly, a reduced value of T 2 * (1.27 6 0.35 ms vs 0.96 6 0.18 ms) was found in the emphysema group. The mean signal intensity drop and the reduction of T 2 * were prominent at 3 weeks following elastase instillation and stabilized between 3 and 8 weeks. The results indicated an excellent agreement between MR findings and histological morphometry (signal intensity, r 5 20.78, P 5 0.004; T 2 *, r 5 20.78, P 5 0.001). This result shows that proton MRI allows structural changes at alveolar level to be monitored longitudinally. This technique, applied routinely in preclinical trials will represent a valuable tool for assessment of drug therapy efficacy. Magn Reson Med 68:898-904,

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Section: Introductionmentioning

confidence: 99%

Longitudinal and noninvasive assessment of emphysema evolution in a murine model using proton MRI

Żurek

Boyer

Caramelle

et al. 2011

Magnetic Resonance in Med

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“…where SI is measured signal intensity, PD is proton density, and N includes background noise (22). The same fitting analysis performed pixel-wise produced parametric maps of proton density and T2*.…”

Section: Proton Density and T2* With Utementioning

confidence: 99%

“…Since UTE-MRI of the lung was first demonstrated in the early 1990s on a 1.5 T system (28), most of the subsequent studies have been performed on animal MR systems at higher field strengths (14,15,18,22). Only recently, investigators performed UTE-MRI of the lung in animal models using clinical systems at 3.0 T (16,17).…”

Section: Te=01 Ms Te=21 Msmentioning

confidence: 99%

“…The UTE sequence offers the possibility of measuring signal at different TE intervals (16,17,22). Thus, signal decay can be observed empirically in the submillisecond time range, during which the majority of lung signal is believed to disappear at an extremely rapid rate.…”

Section: Te=01 Ms Te=21 Msmentioning

confidence: 99%

“…The fitting equation was adapted from the study of Olsson et al (22). The monoexponential fit allowed for consistent calculation of the proton density and T2* of the sponges, and the goodness of the fit was confirmed statistically.…”

Section: Te=01 Ms Te=21 Msmentioning

confidence: 99%

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Ultrashort echo time MRI of pulmonary water content: assessment in a sponge phantom at 1.5 and 3.0 Tesla

Molinari¹,

Madhuranthakam²,

Lenkinski³

et al. 2013

Diagn Interv Radiol

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ORIGINAL ARTICLE PURPOSE We aimed to develop a predictive model for lung water content using ultrashort echo time (UTE) magnetic resonance imaging (MRI) and a sponge phantom. MATERIALS AND METHODSImage quality was preliminarily optimized, and the signalto-noise ratio (SNR) of UTE was compared with that obtained from a three-dimensional fast gradient echo (FGRE) sequence. Four predetermined volumes of water (3.5, 3.0, 2.5, and 2.0 mL) were soaked in cellulose foam sponges 1.8 cm 3 in size and were imaged with UTE-MRI at 1.5 and 3.0 Tesla (T). A multiple echo time experiment (range, 0.1-9.6 ms) was conducted, and the T2 signal decay curve was determined at each volume of water. A three-parameter equation was fitted to the measured signal, allowing for the calculation of proton density and T2*. The calculation error of proton density was determined as a function of echo time. The constants that allowed for the determination of unknown volumes of water from the measured proton density were calculated using linear regression. RESULTS UTE-MRI provided excellent image quality for the four phantoms and showed a higher SNR, compared to that of FGRE. Proton density decreased proportionally with the decreases in both lung water and field strength (from 3.5 to 2.0 mL; proton density range at 1.5 T, 30.5-17.3; at 3.0 T, 84.2-41.5). Minimum echo time less than 0.6 ms at 1.5 T and 1 ms at 3.0 T maintained calculation errors for proton density within the range of 0%-10%. The slopes of the lines for determining the unknown volumes of water with UTE-MRI were 0.12±0.003 at 1.5 T and 0.05±0.002 at 3.0 T (P < 0.0001). CONCLUSIONIn a sponge phantom imaged at 1.5 and 3.0 T, unknown volumes of water can be predicted with high accuracy using UTE-MRI.

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Erratum

2009

Cancer

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Both chemical and topographic cues are crucial for the development of skeletal muscle. In this study, the relative roles of both signals in regard to cell adhesion, morphology, and differentiation of C2C12 skeletal myoblasts were investigated. Grooved polystyrene substrates containing grooves with approximately 900 nm in width with 600 nm ridge spans and 665 nm in depth were conjugated with the cell adhesion peptide arginine–glycine–aspartic acid (RGD). RGD conjugation significantly enhanced the adhesion, growth and differentiation of C2C12 cells. On the other hand, anisotropic topography primarily directed the direction and alignment of myoblasts and myotubes. The results in this study provide information regarding the relative roles of chemical and topographic cues in musculoskeletal myogenesis, and are of interest to applications in muscle tissue engineering. Biotechnol. Bioeng. 2012; 109:2104–2115. © 2012 Wiley Periodicals, Inc.

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Measurement of MR signal and T2* in lung to characterize a tight skin mouse model of emphysema using single‐point imaging

Cited by 52 publications

References 20 publications

Longitudinal and noninvasive assessment of emphysema evolution in a murine model using proton MRI

Longitudinal and noninvasive assessment of emphysema evolution in a murine model using proton MRI

Ultrashort echo time MRI of pulmonary water content: assessment in a sponge phantom at 1.5 and 3.0 Tesla

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