Measurement of plasma cell‐free DNA concentrations in dogs with sepsis, trauma, and neoplasia

Letendre, Jo‐Annie; Goggs, Robert

doi:10.1111/vec.12592

Cited by 45 publications

(67 citation statements)

References 36 publications

(66 reference statements)

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“…A significant increase in the blood concentration of cfDNA was found in diseased dogs, as compared to healthy dogs, and a positive association between cfDNA concentration and disease severity and survival was observed . Both dogs with sepsis and those with moderate‐to‐severe trauma had significantly increased cfDNA concentrations compared to healthy dogs, and cfDNA concentration was associated with death in dogs with immune‐mediated hemolytic anemia …”

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confidence: 94%

“…19,20 The ability to detect cfDNA in canine blood led to the investigation of cfDNA in other disease states. 21,22 A significant increase in the blood concentration of cfDNA was found in diseased dogs, as compared to healthy dogs, and a positive association between cfDNA concentration and disease severity and survival was observed. 21 22 and cfDNA concentration was associated with death in dogs with immune-mediated hemolytic anemia.…”

mentioning

confidence: 98%

“…Dogs with mammary tumors or lymphoma have high concentrations of cfDNA in the blood, and the concentration of cfDNA is inversely correlated with remission time . The ability to detect cfDNA in canine blood led to the investigation of cfDNA in other disease states . A significant increase in the blood concentration of cfDNA was found in diseased dogs, as compared to healthy dogs, and a positive association between cfDNA concentration and disease severity and survival was observed .…”

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confidence: 99%

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Kinetics of Plasma Cell‐Free DNA and Creatine Kinase in a Canine Model of Tissue Injury

Wilson

Burchell

Worth

et al. 2017

Veterinary Internal Medicne

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BackgroundCell‐free DNA (cfDNA) comprises short, double‐stranded circulating DNA sequences released from damaged cells. In people, cfDNA concentrations correlate well with disease severity and tissue damage. No reports are available regarding cfDNA kinetics in dogs.Objectives/HypothesisCell‐free DNA will have a short biological half‐life and would be able to stratify mild, moderate, and severe tissue injury. Our study aims were to determine the kinetics and biological half‐life of cfDNA and to contrast them with those of creatine kinase (CK).AnimalsThree groups of 10 dogs undergoing open ovariohysterectomy, surgery for cranial cruciate ligament rupture (CCLR), or hemilaminectomy.MethodsPlasma for cfDNA and CK analysis was collected at admission, at induction of anesthesia, postsurgery (time 0) and at 6, 12, 24, 36, 48, 60, and 72 hours after surgery.ResultsThe biological half‐life of plasma cfDNA and CK were 5.64 hours (95% confidence interval [CI 95], 4.36–7.98 hours) and 28.7 hours (CI95, 25.3–33.3 hours), respectively. In the hemilaminectomy group, cfDNA concentrations differed significantly from admission at 6–12 hours after surgery. Creatine kinase activity differed among the surgical groups and reached a peak 6 hours after surgery. In the ovariohysterectomy and CCLR groups, plasma CK activity 72 hours after surgery did not differ from admission activity of the ovariohysterectomy group. In contrast, in the hemilaminectomy group, plasma CK activity after 72 hours did not return to the ovariohysterectomy group admission activity.Conclusions and Clinical ImportancePlasma CK activity has a longer biological half‐life than previously thought. In contrast to plasma CK activity, cfDNA has a short half‐life and could be a useful marker for peracute severe tissue injury.

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confidence: 94%

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confidence: 98%

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confidence: 99%

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Kinetics of Plasma Cell‐Free DNA and Creatine Kinase in a Canine Model of Tissue Injury

Wilson

Burchell

Worth

et al. 2017

Veterinary Internal Medicne

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“…In the past, cfDNA was thought to originate largely from cells undergoing apoptosis or necrosis, but in recent years, it has been suggested that DNA might also be released from living cells . Plasma concentrations of cfDNA are increased in various disease states in dogs, including mammary tumors, lymphoid neoplasia, pulmonary thromboembolism, sarcomas, trauma, and sepsis . However, as for NT‐proBNP, the effect of prolonged strenuous exercise on plasma cfDNA concentrations in dogs is unknown.…”

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confidence: 99%

“…17,18 Plasma concentrations of cfDNA are increased in various disease states in dogs, 19 including mammary tumors, 20,21 lymphoid neoplasia, 22 pulmonary thromboembolism, 23 sarcomas, trauma, and sepsis. 24 However, as for NT-proBNP, the effect of prolonged strenuous exercise on plasma cfDNA concentrations in dogs is unknown.…”

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confidence: 99%

Plasma NT‐proBNP and Cell‐Free DNA Concentrations after Prolonged Strenuous Exercise in Working Farm Dogs

Hunt

Cave

Bridges

et al. 2017

Veterinary Internal Medicne

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BackgroundPlasma N‐terminal pro–B‐type natriuretic peptide (NT‐proBNP) concentration is increased in dogs with myocardial dysfunction, and cell‐free DNA (cfDNA) increases in numerous disease states. In humans, both of these biomarkers can be altered after endurance exercise.ObjectiveTo investigate the effect of prolonged strenuous exercise on circulating NT‐proBNP and cfDNA concentrations in working farm dogs.AnimalsSix healthy, privately owned working farm dogs (4 Huntaways and 2 heading dogs) from the same hill country farm in New Zealand.MethodsProspective, nonrandomised cohort study. Venous blood samples were collected before and after the dogs worked over 4 days. Plasma NT‐proBNP concentrations were measured by a commercially available ELISA assay and cfDNA concentrations were determined by fluorometry without prior DNA extraction.ResultsThe baseline (before work, Day 1) median plasma NT‐proBNP concentration was 664 pmol/L. A linear mixed‐effects model showed that work increased plasma NT‐proBNP concentrations by 101 ± 9% (P < 0.001), but with each consecutive day of work, NT‐proBNP concentrations declined by 16 ± 4% (P < 0.001). The baseline median plasma cfDNA concentration was 653 ng/mL, and plasma cfDNA concentrations increased by 138 ± 45 ng/mL after work (P = 0.004).Conclusions and Clinical ImportanceThe plasma concentration of NT‐proBNP in healthy Huntaways and heading dogs after work can exceed the upper limit of the reference range. Results in dogs sampled on the day of prolonged strenuous exercise should be interpreted with caution. Plasma concentrations of cfDNA also increase with exercise, but further studies are needed to establish reference ranges in healthy dogs.

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Single-step measurement of cell-free DNA for sepsis prognosis using a thread-based microfluidic device

et al. 2022

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Measurement of plasma cell‐free DNA concentrations in dogs with sepsis, trauma, and neoplasia

Abstract: cfDNA can be readily identified in canine plasma using 2 fluorescence assays. DNA extraction offers no advantage over direct measurement. Compared to healthy controls, dogs with sepsis or moderate-severe trauma have significantly increased plasma cfDNA concentrations.

Cited by 45 publications

References 36 publications

Kinetics of Plasma Cell‐Free DNA and Creatine Kinase in a Canine Model of Tissue Injury

Kinetics of Plasma Cell‐Free DNA and Creatine Kinase in a Canine Model of Tissue Injury

Plasma NT‐proBNP and Cell‐Free DNA Concentrations after Prolonged Strenuous Exercise in Working Farm Dogs

Single-step measurement of cell-free DNA for sepsis prognosis using a thread-based microfluidic device

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