Measurement of plasma corticosterone and fecal glucocorticoid metabolites in the chicken (Gallus domesticus), the great cormorant (Phalacrocorax carbo), and the goshawk (Accipiter gentilis)

Dehnhard, Martin; Schreer, A.; Krone, Oliver; Jewgenow, Katarina; Krause, M.; Großmann, Roland

doi:10.1016/s0016-6480(03)00033-9

Cited by 125 publications

(100 citation statements)

References 31 publications

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“…Möstl and Palme, 2002;Millspaugh and Washburn, 2004;Goymann, 2005;Möstl et al, 2005;Palme et al, 2005;Touma and Palme, 2005). A small number of studies have simultaneously measured plasma hormones and faecal hormone metabolites to detect changes following artificial stimulation and suppression in domestic animals Palme et al, 1999;Dehnhard et al, 2003); however, to our knowledge no studies have specifically quantified the relationship between plasma and faecal measures in a natural context, nor examined the consistency of results obtained when plasma hormone and faecal hormone metabolite measures are used simultaneously to assess baseline hormone concentrations. Ultimately, plasma and faecal measures appear suited for application to quite different questions.…”

Section: Introductionmentioning

confidence: 99%

Comparing plasma and faecal measures of steroid hormones in Adelie penguins Pygoscelis adeliae

et al. 2009

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Abstract. Physiological measurements of both stress and sex hormones are often used to estimate the consequences of natural or human-induced change in ecological studies of various animals. Different methods of hormone measurement exist, potentially explaining variation in results across studies; methods should be cross-validated to ensure they correlate.We directly compared faecal and plasma hormone measurements for the first time in a wild free-living species, the Adelie penguin (Pygoscelis adeliae). Blood and faecal samples were simultaneously collected from individual penguins for comparison, and assayed for testosterone and corticosterone (or their metabolites). Sex differences and variability within each measure, and correlation of values across measures were compared. For both hormones, plasma samples showed greater variation than faecal samples. Males had higher mean corticosterone concentrations than females, but the difference was only statistically significant in faecal samples. Plasma testosterone, but not faecal testosterone, was significantly higher in males than females. Correlation between sample types was poor overall, and weaker in females than in males, perhaps because measures from plasma represent hormones that are both free and bound to globulins, whereas measures from faeces represent only the free portion. Faecal samples also represent a cumulative measure of hormones over time, as opposed to a plasma 'snapshot' concentration. Our data indicate that faecal sampling appears more suitable for assessing baseline hormone concentrations, while plasma sampling may best define immediate responses to environmental events. Consequently, future studies should ensure they select the most appropriate matrix and method of hormone measurement to answer their research questions.

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Section: Introductionmentioning

confidence: 99%

Comparing plasma and faecal measures of steroid hormones in Adelie penguins Pygoscelis adeliae

et al. 2009

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“…Non-invasive methods originally developed for research into animal welfare (Wasser et al 2000; have become a tool in ecology and conservation biology (Nakagawa et al 2003;Baltic et al 2005;Walker et al 2005;Thiel et al 2008). Enzyme immunoassays (EIA) that measure faecal glucocorticoid (GC) metabolites have enabled researchers to associate behavioural changes in free-ranging animals with the physiological costs they generate and to quantify the impact of natural or anthropogenic-induced stress (Kotrschal et al 1998;Dehnhard et al 2003;Quillfeldt and Möstl 2003;Tempel and Gutiérrez 2004).…”

Section: Introductionmentioning

confidence: 99%

Non-invasive measurement of faecal glucocorticoid metabolites in Upland Geese Chloephaga picta

et al. 2008

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Glucocorticoid (GC) hormones rise in response to stressors, including natural events including weather or predator presence, and human activities, such as hunting, scientiWc research or recreational visits. However, because blood sampling itself causes stress and is dangerous or even impossible in some wildlife species, feedback-free methods for GC determination are needed to assess stress in these animals. Faecal GC analyses have thus gained interest. Here, we validate a non-invasive method to estimate the physiological stress in the Upland goose Chloephaga picta. An adrenocorticotropin hormone (ACTH) challenge was conducted in captive adults (female and male), and droppings were collected before, during and after the experiment. Corticosterone metabolite (CM) secretion in response to the ACTH challenge was measured with several enzyme immunoassays (EIA) to Wnd the most appropriate test. We used CM levels during the periods before and after the experiment as control data. An EIA for 11-oxoetiocholanolone achieved the highest response to the ACTH challenge and also reXected a stress response to unfamiliar environment. Furthermore, CM concentrations of dry samples were highly correlated with the corresponding non-dried (frozen) samples. The data suggest that this method is appropriate to measure the stress in Upland geese, and that samples can be stored either frozen or dry form.

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“…As modificações das concentrações fecais de MG observadas no presente estudo foram semelhantes às observadas por Lee et al 19 , que relataram pico de excreção de hormônios esteroides após quatro horas da administração de esteroides radiomarcados em dois gêneros de psitaciformes (Melopsittacus undulates e Amazona amazonica), e por Wasser et al 9 , Denhard et al 11 e Thiel et al 20 que, após administração de ACTH, observaram, respectivamente, picos de excreção às duas e às 12 horas em duas espécies de corujas (Strix vari e Bubo virginianus), aumento de excreção de metabólitos de glucocorticóides após quatro horas em galinhas (Gallus domesticus), corvo (Phalacrocorax carbo) e açor (Accipiter gentilis) e variação individual nos picos de excreção em tetraz-grande (Tetrao urogallus). Quando considerados os resultados obtidos na dosagem de MG por RIA, empregando-se anticorpo contra corticosterona, apesar da ausência de modificações significativas após a administração de ACTH ou solução salina, também foram observados picos de excreção; contudo, esses picos foram assincrônicos em relação aos observados na dosagem por EIA empregando-se anticorpo contra cortisol.…”

Section: Discussionunclassified

“…Entre os diversos métodos de monitoramento, destaca-se a dosagem hormonal, por meio da qual pode-se observar o nível de estresse nos animais, entre outros 9 . Em amostras fecais, para que as dosagens possam ser realizadas, torna-se necessária a validação das técnicas de quantificação hormonal, pois a forma e o tempo de excreção dos metabólitos dos hormônios variam consideravelmente entre as espécies [9][10][11][12] .…”

Section: Introductionunclassified

Dosagem de metabólitos de glucocorticoides e progesterona em fezes de papagaio-verdadeiro (Amazona aestiva)

Fujihara¹,

Filho²,

Monteiro³

et al. 2014

Ciênc. anim. bras.

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RESUMOOs objetivos do presente trabalho foram avaliar as concentrações fecais de metabólitos de glicocorticoides, mensurados por enzimaimunoensaio, empregando-se anticorpos contra cortisol, e por radioimunoensaio, empregando-se anticorpo contra corticosterona, e dos metabólitos da progesterona, mensurados por raioimunoensaio empregando-se anticorpo contra progesterona, em papagaios-verdadeiros (Amazona aestiva) após desafio com ACTH. A estimulação da adrenal com 25 UI/animal de ACTH resultou na elevação das concentrações de metabólitos de glicocorticoides, mas não modificou a dos metabólitos da progesterona fecal em papagaio-verdadeiro. Embora não tenha sido observada a sincronização dos picos de excreção fecal dos metabólitos de glicocorticoides mensurados por enzimaimunoensaio e radioimunoensaio, houve dois picos de excreção, um entre 2 e 4 horas e outro entre 8 e 10 horas. Apesar dos picos, não foram detectados efeitos de Grupos (Tratamento x Controle), momento (horas de coleta) ou sexo (macho x fêmea) nos resultados observados nas concentrações fecais de metabólitos de glicocorticoides e de progesterona, com os métodos empregados. PALAVRAS-CHAVE: aves, corticosterona, cortisol, não-invasivo, progesterona. MEASUREMENT OF GLUCOCORTICOID AND PROGESTERONE METABOLITES IN FECES OF BLUE FRONTED PARROT (Amazona aestiva) ABSTRACTThe objectives of the present study were to evaluate fecal concentrations of metabolites of glucocorticoids, measured by enzyme immunoassay with a cortisol antibody and by radioimmunoassay with a corticosterone antibody, and progesterone by radioimmunoassay with a progesterone antibody in blue-fronted parrot (Amazona aestiva) after ACTH challenge. The adrenal stimulation with ACTH (25 UI/animal) resulted in an increase of fecal glucocorticoids metabolites concentration, but it did not affect the concentrations of fecal progesterone metabolites. Although there were no synchronized peaks of glucocorticoid metabolites excretion measured by enzyme immunoassay and radioimmunoassay, there were two peaks of excretion, one at 2-4 hours and other at 8-10 hours. Despite the occurrence of peaks, the analysis of fecal glucocorticoids metabolites and progesterone

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Measurement of plasma corticosterone and fecal glucocorticoid metabolites in the chicken (Gallus domesticus), the great cormorant (Phalacrocorax carbo), and the goshawk (Accipiter gentilis)

Cited by 125 publications

References 31 publications

Comparing plasma and faecal measures of steroid hormones in Adelie penguins Pygoscelis adeliae

Comparing plasma and faecal measures of steroid hormones in Adelie penguins Pygoscelis adeliae

Non-invasive measurement of faecal glucocorticoid metabolites in Upland Geese Chloephaga picta

Dosagem de metabólitos de glucocorticoides e progesterona em fezes de papagaio-verdadeiro (Amazona aestiva)

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