Measurement of plasma renin activity by semi-micro radioimmunoassay of generated angiotensin I.

Dillon, M J

doi:10.1136/jcp.28.8.625

Cited by 31 publications

(14 citation statements)

References 16 publications

(16 reference statements)

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“…Urinary albumin was measured using a commercial ELISA kit (Randox Laboratories) where the sensitivity was 0.1 rag/1 and intra-and interassay CVs were 6% and 9%. PRA was measured by a semi-micro radio-immunoassay [18] and the intra-and interassay CVs were 5% and 10%-15%, respectively. Plasma and urine creatinines were measured utilising standard enzymatic and Jaff6 reactions, respectively.…”

Section: Methodsmentioning

confidence: 99%

Tubular proteinuria in reflux nephropathy: post ureteric re-implantation

1996

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We studied urine protein excretion in 55 adults with reflux nephropathy (median age 26.9 years) who had had normal blood pressure, renal function and ureteric reimplantation in childhood. Urine retinol binding protein (RBP), N-acetyl-beta-D-glucosaminidase (NAG), albumin, bacteriuria, systolic blood pressure, glomerular filtration rate (GFR), peripheral plasma renin activity (PRA) and the degree of renal scarring were measured in each subject; 20 had bilateral and 35 unilateral renal scarring; 5 were hypertensive and none were in renal failure. Urinary NAG and RBP excretions were significantly greater in the study group than in 34 healthy controls (median age 29.7 years). Within the study group, NAG excretion significantly correlated with PRA (P = 0.02). RBP excretion correlated with PRA, systolic blood pressure and the laterality (bilateral vs. unilateral) of scarring (P < 0.01). Urinary albumin excretion correlated with systolic blood pressure (P = 0.03). We conclude that increased urinary protein, especially NAG and RBP excretion, occur late after ureteric re-implantation in reflux nephropathy independent of GFR. Its association with PRA supports the concept of segmental perfusion and filtration as an important mechanism that may explain the above findings.

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Section: Methodsmentioning

confidence: 99%

Tubular proteinuria in reflux nephropathy: post ureteric re-implantation

1996

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“…Plasma renin activity was measured in venous blood after the patient had 2 h bedrest by radioimmunoassay of angiotensin I generated (Dillon et al, 1975). A midstream urine was obtained for urine analysis and culture.…”

Section: Patient Characteristics and Methodsmentioning

confidence: 99%

Renal size and function after cure of Wilms' tumour

Levitt

Yeomans²,

Mireaux³

et al. 1992

Br J Cancer

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“…), throughout the 25 years [24,25]. The within-assay variation was ±5%, and hence the maximum PRA ratio between identical samples caused by imprecision in the assay was 1.1.…”

Section: Methodsmentioning

confidence: 98%

The usefulness of renal vein renin studies in hypertensive children: a 25-year experience

Goonasekera

Shah

Wade

et al. 2002

Pediatric Nephrology

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Lateralised renin secretion predicts improvement following surgery/angioplasty of the affected kidney in paediatric hypertension. We retrospectively analysed all our renal vein renin (RVR) studies undertaken in hypertensive children and their subsequent clinical outcomes over a 25-year period. The outcomes were categorised as cure, improvement or no change in hypertension. The proportions that benefited by 'following' or 'ignoring' the RVR results in relation to the aetiology of hypertension and subsequent therapy were compared. A ratio between the RVR value of the dominant kidney and that of the contralateral kidney (R/Rc ratio) above 1.5 was considered to show significant lateralisation. A ratio between RVR value of the contralateral kidney and that of the caudal inferior vena cava (Rc/P ratio) of less than 1.3 was considered to indicate contralateral renin suppression. Of 233 RVR records, 137 were suitable for analysis. Of these, 39 had reflux nephropathy (RN), 73 renovascular disease (RVD) and the others miscellaneous conditions. Of the 39 hypertensive children with RN, the R/Rc ratio was over 1.5 in 20 subjects, of whom 11 underwent surgery. Amongst the 19 with an R/Rc less than 1.5, 4 also had surgery. Of the 73 hypertensive children with RVD, the R/Rc ratio was over 1.5 in 38 subjects, of whom 20 underwent surgery/angioplasty. Amongst the 35 with an R/Rc less than 1.5, 7 also underwent surgery/angioplasty. In RN there was no significant difference in the proportion that improved with allocated treatment, as suggested by the 'test' when compared with those who were 'misallocated'. In RVD, however, the proportion improved (35.0%) by 'following the test' (i.e. R/Rc ratio) was significantly greater than the proportion improved (12.5%) in the group in whom the test was 'ignored'. This was true for both R/Rc and Rc/P ratios. Thus, RVR measurements are useful for identifying hypertensive children with RVD that may have a favourable outcome after surgery, but this does not apply to children with RN. Measurements, however, may have diagnostic value even if surgery or angioplasty is not anticipated.

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Measurement of plasma renin activity by semi-micro radioimmunoassay of generated angiotensin I.

Cited by 31 publications

References 16 publications

Tubular proteinuria in reflux nephropathy: post ureteric re-implantation

Tubular proteinuria in reflux nephropathy: post ureteric re-implantation

Renal size and function after cure of Wilms' tumour

The usefulness of renal vein renin studies in hypertensive children: a 25-year experience

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