Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification

Nam, Jungchan; Yoo, Minyoung; Yeo, Woon‐Seok

doi:10.1007/s13206-015-9205-4

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…After this, they can easily be detected by MS that results in an amplified signal of the targets. The combination of mass barcodes and magnetic beads has been proved to be a very effective for MS detection of large biomolecules, e.g., allergens (Nam et al, 2015).…”

Section: Barcoding and Detection Of Allergensmentioning

confidence: 99%

A Spellbinding Interplay Between Biological Barcoding and Nanotechnology

Munir

Ahmed

Ibrahim

et al. 2020

Front. Bioeng. Biotechnol.

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Great scientific research with improved potential in probing biological locales has remained a giant stride. The use of bio-barcodes with the potential use of nanotechnology is a hallmark being developed among recent advanced techniques. Biobarcoding is a novel method used for screening biomolecules to identify and divulge ragbag biodiversity. It establishes successful barcoding projects in the field of nanomedical technology for massively testing disease diagnosis and treatment. Biobarcoding and nanotechnology are recently developed technologies that provide unique opportunities and challenges for multiplex detection such as DNAs, proteins and nucleic acids of animals, plants, viruses, and various other species. These technologies also clump drug delivery, gene delivery, and DNA sequencing. Bio-barcode amplification assay (BCA) is used at large for the detection and identification of proteins and DNAs. DNA barcoding combined with nanotechnology has been proven highly sensitive rendering fast uniplex and multiplex detection of pathogens in food, blood, and other specimens. This review takes a panoramic view of current advances in nano biobarcodes which have been summarized to explore additional applications such as detection of cytokines, neurotransmitters, cancer markers, prostate-specific antigens, and allergens. In the future, it will also be possible to detect some fungi, algae, protozoa, and other pollutants in food, agriculture, and clinical samples. Using these technologies, specific and efficient sensors would possibly be developed that can perform swift detections of antigens, allergens, and other specimens.

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Section: Barcoding and Detection Of Allergensmentioning

confidence: 99%

A Spellbinding Interplay Between Biological Barcoding and Nanotechnology

Munir

Ahmed

Ibrahim

et al. 2020

Front. Bioeng. Biotechnol.

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“…More recently, Yeo's group further applied the same approach for the detection of a tumor marker, prostate specic antigen (PSA) in human serum samples, with a linear dynamic range from 10 fM to 5 pM. 18 Du et al reported aptamer and hexa(ethylene glycol)-co-modied Au NPs, which allow detection of thrombin and EpCAM (epithelial cell adhesion molecule), with LODs on the sub-femtomolar scale, through a sandwich assay coupled with an LDI-MS detection platform (Fig. 3A).…”

Section: Detection Of Proteinsmentioning

confidence: 99%

“…1c,6c,10a,10b,13 However, these reviews discuss the use of nanomaterials as matrices for LDI-MS analysis. A considerable number of articles on the LDI-MS analysis of biomolecules, as well as inorganic analytes, by monitoring the surface ligands of Au NPs [14][15][16][17][18][19][20][21][22][23][24] or gold cluster ions have been published. [25][26][27][28][29][30][31][32][33][34] Although a mini-review published in 2009 by Vachet et al discussed the application of the surface ligands of Au NPs as mass tags in LDI-MS for DNA and protein analysis, 35 reviews on various aspects of surface ligand ions and gold cluster ions as indirect methods for bioanalysis have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Functional gold nanoparticles coupled with laser desorption ionization mass spectrometry for bioanalysis

et al. 2016

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“…This mass signal amplification strategy uses organic-matrix-free laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) and gold nanoparticles (AuNPs). [12][13][14][15] In the present study, we extended this method with a multiplexing capability to detect the two types of serum PSA and quantify their ratio in complex samples with tPSA levels between 4 and 10 ng/mL. Our strategy combines two antibodies that recognize the PSA epitopes with two amplification tags (Am-tags), one of which is deuterium labeled.…”

Section: Introductionmentioning

confidence: 99%

Determining the Ratio of Two Types of Prostate Specific Antigens with Biochips and Gold Nanoparticles for Accurate Prostate Cancer Diagnosis

Yoo

Yeo

2016

ANAL. SCI.

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Prostate-specific antigen (PSA) is a well-known biomarker for prostate-cancer diagnosis. However, the serum PSA measurement alone is insufficient for accurate diagnoses because the correlation with cancer is weak within the gray zone-the biomarker level range wherein a clear-cut diagnosis is impossible. As such, accurate prostate cancer diagnosis has been supplemented by measurements of the ratio of two types of PSA: free PSA (fPSA) and complexed PSA (cPSA; α-1-antichymotrypsin-bound PSA). Herein, we describe a new method for measuring the ratio of these two types of PSA by using gold nanoparticles (AuNPs) and biochips. Both types of PSA in a sample are captured by the antibody immobilized on a biochip based on self-assembled monolayers on gold. fPSA and cPSA on the biochip are then distinguished by AuNPs that present antibodies against fPSA and cPSA, respectively. The presence of PSAs in a sample is detected with laser desorption/ionization time-of-flight mass spectrometry by observing reporter molecules, called amplification tags (Am-tags), on the AuNPs. One of the reporter molecules is an Am-tag without isotope labeling, and the other is a deuterium-labeled Am-tag (dAm-tag). These tags amplify mass signals so as to enhance the sensitivity of the method. A comparison of the mass intensities between the Am-tag and dAm-tag signals allows the determination of the ratio between fPSA and cPSA. We validated the selective measurement of fPSA and cPSA at different ratios in 50, 75, and 100 pM of total PSA (fPSA + cPSA) solutions corresponding to the gray zone in prostate-cancer diagnosis (4 - 10 ng/mL). Finally, the two types of PSA were spiked in fetal bovine serum at various ratios, and our strategy greatly afforded their accurate ratios as spiked based on a constructed calibration curve. These results clearly indicate that the strategy is applicable to human serum as a diagnostic and prognostic assay for prostate cancer.

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Measurement of prostate-specific antigen level as a biomarker for breast cancer by using mass signal amplification

Cited by 10 publications

References 33 publications

A Spellbinding Interplay Between Biological Barcoding and Nanotechnology

A Spellbinding Interplay Between Biological Barcoding and Nanotechnology

Functional gold nanoparticles coupled with laser desorption ionization mass spectrometry for bioanalysis

Determining the Ratio of Two Types of Prostate Specific Antigens with Biochips and Gold Nanoparticles for Accurate Prostate Cancer Diagnosis

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