The fabrication technique of deformation carriers with focused ion beam (FIB) milling for microscale/nanoscale deformation measurement has been studied in this chapter. The deformation carriers refer to microscale/nanoscale gratings and speckle patterns, which are indispensable elements for moiré and digital image correlation (DIC) method under scanning electron microscope (SEM) respectively. The fabrication principle, the design, and the influencing factors of these two kinds of deformation carriers are studied respectively. Their successful applications to microscale/ nanoscale deformation measurement are also presented, which demonstrate that the deformation carriers combined with photo-mechanics techniques under SEM are effective tools for microscale/nanoscale deformation measurement.