Measurement of SCE frequencies in plants: a simple Feulgen-staining procedure for Vicia faba

Tempelaar, M. J.; Both, Michiel T. J. de; Versteegh, J.E.G.

doi:10.1016/0165-7992(82)90061-6

Cited by 20 publications

(9 citation statements)

References 5 publications

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“…In fact, Tempelaar et al (1982) noted that when plants with a high mitotic index were used, the yield of plants showing sister chromatid differentiation could be doubled, suggesting a relationship between more actively dividing root meristems and increased BrdU uptake.…”

Section: Factors Affecting the Mitotic Indexmentioning

confidence: 99%

“…(Vosa 1981, Tempelaar et al 1982. Since BrdU substituted DNA resists acid hydrolysis compared to unsubstituted DNA, there is a specific duration of acid hydrolysis treatment which gives rise to good differential contrast.…”

Section: Factors Affecting the Mitotic Indexmentioning

confidence: 99%

“…Of the approximately 76 papers which fall into this category, two have been published in which the Feulgen technique is used (Vosa 1981, Tempelaar et al 1982. In both papers, staining is carried out in Vicia faba.…”

Section: Factors Affecting the Mitotic Indexmentioning

confidence: 99%

“…Kihlman and Sturelid 1978, Andersson et al 1981, Andersson 1981, 1985. (Kihlman 1975b, Tempelaar et al 1982, Kihlman and Andersson 1984), and (6) loss of differential staining due to the effect of impurities which may be present in these enzymes ( Kilhman and Andersson 1984). Kihlman and Kronborg (1975) noted that irregularities in the staining of plant chro mosomes may be a problem with Giemsa, while other workers (Scheid 1976, Scheid andTraupe 1977) failed to obtain sister chromatid differentiation in Vicia faba without the prior proteolytic digestion of the chromosomes with trypsin.…”

Section: Studiesmentioning

confidence: 99%

“…In the present study, this range was found to be between 80 and 86min. SO2 wash: In Tempelaar et al (1982) the maceration of root tips is followed by a 10min wash in SO2 water to remove excess Feulgen reagent. In the present study, no difference was seen between preparations with or without SO2 wash.…”

Section: Factors Affecting the Mitotic Indexmentioning

confidence: 99%

See 4 more Smart Citations

A cytogenetic study of factors affecting sister chromatid differentiation in Vicia faba and Hordeum vulgare.

Gerster

Grant

1989

CYTOLOGIA

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Section: Factors Affecting the Mitotic Indexmentioning

confidence: 99%

Section: Factors Affecting the Mitotic Indexmentioning

confidence: 99%

Section: Factors Affecting the Mitotic Indexmentioning

confidence: 99%

Section: Studiesmentioning

confidence: 99%

Section: Factors Affecting the Mitotic Indexmentioning

confidence: 99%

See 3 more Smart Citations

A cytogenetic study of factors affecting sister chromatid differentiation in Vicia faba and Hordeum vulgare.

Gerster

Grant

1989

CYTOLOGIA

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Sister chromatid exchange in human lymphocytes induced by propoxur following plant activation by Vicia faba

Gómez‐Arroyo¹,

Calderón-Segura²,

Villalobos‐Pietrini³

1995

Environ and Mol Mutagen

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Because the carbamate insecticide propoxur induced sister chromatid exchanges (SCE) in Vicia faba but was ineffective in producing SCE in lymphocytes in culture, it was hardly suspected that plant metabolism was involved. Experiments were conducted in which metabolic activation was afforded by Vicia faba roots, and SCE in human lymphocytes in vitro was used to assess cytogenetic damage. Several concentrations of propoxur (250, 500, 1,000, 1,500, and 2,000 ppm) were applied for 4 hr to the roots of Vicia faba. Extracts prepared from these treatments were added to the lymphocyte cultures and a significant increase of SCE frequencies with a concentration-response relationship could be detected. The lymphocyte proliferation kinetics and the proliferation rate index (PRI) were not affected (except in the highest concentration, of 2,000 ppm). This general behavior was in agreement with the presence of an enzymatic system (S10 fraction) in Vicia roots capable of metabolizing or activating the propoxur. With 2,000 ppm, cell necrosis was produced in Vicia; therefore, this extract did not induce SCE in lymphocytes. However, lymphocyte proliferation kinetics were delayed and PRI was significantly decreased. Ethanol, a promutagen activated by this plant, was applied directly to the lymphocyte cultures as a positive control, and the response was negative. On the other hand, the extracts of roots treated with ethanol increased the SCE to more than twice that of the negative control, but the lymphocyte proliferation kinetics and PRI were not affected.

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Biomonitoring of Pesticides by Plant Metabolism: An Assay Based on the Induction of Sister-Chromatid Exchanges in Human Lymphocyte Cultures by Promutagen Activation of Vicia faba

Gómez‐Arroyo

Calderón-Segura

Villalobos‐Pietrini

2001

Biomonitors and Biomarkers as Indicators of Environmental Change 2

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Measurement of SCE frequencies in plants: a simple Feulgen-staining procedure for Vicia faba

Cited by 20 publications

References 5 publications

A cytogenetic study of factors affecting sister chromatid differentiation in Vicia faba and Hordeum vulgare.

A cytogenetic study of factors affecting sister chromatid differentiation in Vicia faba and Hordeum vulgare.

Sister chromatid exchange in human lymphocytes induced by propoxur following plant activation by Vicia faba

Biomonitoring of Pesticides by Plant Metabolism: An Assay Based on the Induction of Sister-Chromatid Exchanges in Human Lymphocyte Cultures by Promutagen Activation of Vicia faba

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