Measurement of serum and peritoneal fluid LH concentrations as a diagnostic tool for human endometriosis

Abstract

A rapid, sensitive enzymeimmunoassay for the measurement of LH concentrations in serum and peritoneal fluid samples of healthy women and women with endometriosis is reported. The ligand (LH) was captured by a readily available, widely used and well-characterized monoclonal antibody (mAb, 518B7) generated against the beta subunit of bovine LH. This mAb, although specific for LH, shows very little species specificity and detects LH by radioimmunoassay in humans. A polyclonal antiserum raised in rabbits against hCG was conjugated to horseradish peroxidase and was used as the second antibody signal. This anti-hCG antiserum crossreacts with LH. The enzymeimmunoassay uses the standard human LH (hLH) preparations (NIADDK-hLH-I-3, AFP-827OB) and results are based on the relative concentrations of LH in serum and peritoneal fluid. Total assay time was < 3 h. The range of the standard curve was 0.002-0.500 ng LH per well and the lowest concentration of hLH that could be distinguished from zero concentration was 0.15 +/- 0.02 ng ml(-1) serum and 0.058 +/- 0.021 ng ml(-1) peritoneal fluid. Clinical diagnostic parameters for the LH enzymeimmunoassay showed a sensitivity of 85.71%, specificity 92.50%, efficiency 88.54%, positive predictive value 94.11% and negative predictive value 82.22%. The study was retrospective. Serum LH concentrations of women with endometriosis were 13.67 +/- 7.21 ng ml(-1), whereas serum LH concentrations of women in the control group were 4.52 +/- 2.03 ng ml(-1). One-way ANOVA showed significant differences (P < 0.001) between women with endometriosis and control groups. Women in the control group had peritoneal fluid LH values of 5.65 +/- 2.43 ng ml(-1), whereas peritoneal fluid LH values of 64.06 +/- 16.44 ng ml(-1) were obtained in women with endometriosis (P < 0.001). A cycle-dependent pattern of serum and peritoneal fluid LH concentration was observed in women in the control group, which was not observed in the peritoneal fluid of the group with endometriosis. The application of this assay to serum or peritoneal fluid samples provides the attractive possibility that it could be included in the panel of markers used for diagnosis of endometriosis.

Regulation and distribution of squirrel monkey chorionic gonadotropin and secretogranin II in the pituitary

“…The luciferase reporter gene containing −869/+1 of the human SgII promoter has been described previously [27]. The 518B7 anti-bovine LH monoclonal antibody was provided by Dr. Janet F. Roser (University of California, Davis), and has been shown to react with human LH and marmoset CG [14, 24, 25, 33]. Rabbit polyclonal antibody to human SgII [35] was affinity-purified using a peptide corresponding to the first nineteen N-terminal amino acids of mature SgII protein, QRNQLLGKEPDLRLENVQK (United States Biologicals, Swampscott, MA).…”

Aprendizaje - Servicio (ApS). Metodología y aplicación desde la asignatura de Gestión y Control de la Calidad = Service-Learning (S-L). Methodology and application from the subject of Quality Management and Control

“…En general, no es sencillo combinar estos tres elementos con éxito desde el principio debido a Se ha elegido esta asignatura y no otras distintas debido a la transversalidad e importancia de la Calidad en el desempeño del ejercicio profesional de ingenieros/as y arquitectos/as. Y es que, la calidad debe situarse como una de las variables estratégicas de la empresa, en la medida en que incrementar el grado de satisfacción de los clientes, equivale a mejorar la situación competitiva de las empresas [16]. En concreto, para la realización de esta experiencia se ha tenido en consideración la rama de la Calidad que guarda relación con la ecoeficiencia, en total relación con los Objetivos para el Desarrollo Sostenible de la Agenda 2030.…”

Preliminary Findings on How Different Management Systems and Social Interactions Influence Fecal Glucocorticoid Metabolites in White Rhinoceros (Ceratotherium simum)

“…Under a stressful situation, organisms undergo some changes to adapt to and deal with the new challenge. The release of cortisol is part of this physiological adaptation, an essential unspecific response for the organism to cope with stressful stimuli and survive [ 9 , 16 , 17 , 18 ]. Glucocorticoids, similar to cortisol, are crucial components in evaluating both acute and chronic stress responses, offering substantial information about how animals perceive and adapt to their surroundings [ 18 , 19 , 20 ].…”