Measurement of serum immunoglobulin G in dairy cattle using Fourier-transform infrared spectroscopy: A reagent free approach

Elsohaby, Ibrahim; Riley, Christopher B.; Hou, Siyuan; McClure, J. Trenton; Shaw, R. Anthony; Keefe, Gregory P.

doi:10.1016/j.tvjl.2014.09.014

Cited by 24 publications

(31 citation statements)

References 36 publications

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“…4). The same results were reported during PLS model building for IgG concentrations of bovine, 14 equid, 21 camelid, 3 and canine samples. 40 The lower precision of the ATR-IR method is likely associated with the variability in sample dryness for each replicate, and to the low number of ATR-IR scans (32 scans), as compared to the TIR method (512 scans).…”

Section: Discussionsupporting

confidence: 82%

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Quantification of bovine immunoglobulin G using transmission and attenuated total reflectance infrared spectroscopy

et al. 2015

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Abstract. In this study, we evaluated and compared the performance of transmission and attenuated total reflectance (ATR) infrared (IR) spectroscopic methods (in combination with quantification algorithms previously developed using partial least squares regression) for the rapid measurement of bovine serum immunoglobulin G (IgG) concentration, and detection of failure of transfer of passive immunity (FTPI) in dairy calves. Serum samples (n = 200) were collected from Holstein calves 1-11 days of age. Serum IgG concentrations were measured by the reference method of radial immunodiffusion (RID) assay, transmission IR (TIR) and ATR-IR spectroscopy-based assays. The mean IgG concentration measured by RID was 17.22 g/L (SD ±9.60). The mean IgG concentrations predicted by TIR and ATR-IR spectroscopy methods were 15.60 g/L (SD ±8.15) and 15.94 g/L (SD ±8.66), respectively. RID IgG concentrations were positively correlated with IgG levels predicted by TIR (r = 0.94) and ATR-IR (r = 0.92). The correlation between 2 IR spectroscopic methods was 0.94. Using an IgG concentration <10 g/L as the cut-point for FTPI cases, the overall agreement between TIR and ATR-IR methods was 94%, with a corresponding kappa value of 0.84. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy for identifying FTPI by TIR were 0.87, 0.97, 0.91, 0.95, and 0.94, respectively. Corresponding values for ATR-IR were 0.87, 0.95, 0.86, 0.95, and 0.93, respectively. Both TIR and ATR-IR spectroscopic approaches can be used for rapid quantification of IgG level in neonatal bovine serum and for diagnosis of FTPI in dairy calves.

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Section: Discussionsupporting

confidence: 82%

“…Previously developed PLS models built for prediction of IgG concentration from TIR spectra 14 and ATR-IR spectra 13 were used to predict serum IgG concentrations in the 203 calf samples. These PLS models were built using 200 serum samples collected from calves and adult cows.…”

Section: Prediction Of Iggmentioning

confidence: 99%

Quantification of bovine immunoglobulin G using transmission and attenuated total reflectance infrared spectroscopy

et al. 2015

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Section: Introductionmentioning

confidence: 99%

“…It shows promise for the analysis of lipid quality parameters, and has several advantages such as minimizing the preparation stage of the sample and allowing direct analyses without the use of organic. Moreover, it allows determination of several oil quality parameters simultaneously (Elsohaby et al, 2014;Talpur et al, 2015). Chen, Zhang, Ma, Tuchiya, and Miao (2015) utilized out several fries of potatoes in a controlled environment, and used the multivariate calibration to predict acidity values and polar compound content of frying oils using near-infrared spectroscopy.…”

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confidence: 99%

At‐line monitoring of industrial frying processes using ATR‐FTIR‐PLS method

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Filoda

Tischer

et al. 2018

J Food Process Engineering

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This study investigated the application of infrared spectroscopy with multivariate calibration methods for at‐line monitoring of the degradation of soybean oil in industrial frying processes by determining when the acidity index and total polar materials (TPM). The infrared spectra (650–3,200 cm−1) were acquired using the attenuated total reflection accessory (ATR‐FTIR), with a resolution of 4 cm−1, and 16 scans. Partial least‐squares regression (PLS) models were evaluated for individual and simultaneous determination, and results were compared with reference methods. The individual calibration model showed standard error of prediction values of 0.09% (w/w) and 1.6% (w/w) for the acidity index and TPM, respectively. The simultaneous determination of the acidity index and TPM showed SEP values of 0.17% (w/w) and 1.6% (w/w), respectively. The results demonstrate that infrared spectroscopy combined with multivariate calibration techniques can be servant used in routine soybean oil quality control in industrial frying processes. Practical applications Foods prepared by the frying process are strongly influenced by the oil used in the process, since the product tends to absorb part of the oil during its cooking. This oil when used for a long period and exposed to high temperatures, end up suffering oxidation reactions, and can greatly reduce your shelf life. In this way the industries that use these processes for the manufacture of their products, apply a series of chemical analyzes in order to validate the quality of the oil used, these analyzes use a high amount of solvents and time. As an alternative to the use of these traditional analyzes, methods have been developed through the use of infrared spectroscopy combined with multivariate analysis tools, for the creation of partial least squares calibration models for the prediction of quality parameters of oils used in frying.

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“…In comparison, ATR-IR spectroscopy by its nature does not have issues associated with optical path length or sample thickness. Previous reports have illustrated the use of transmission-IR spectroscopy in combination with PLSR to quantify IgG concentrations in bovine [23, 24], equine [25] and camelid species [26]. However, it is less amenable for field use on the farm, veterinary clinics or small laboratories.…”

Section: Introductionmentioning

confidence: 99%

Application of laboratory and portable attenuated total reflectance infrared spectroscopic approaches for rapid quantification of alpaca serum immunoglobulin G

et al. 2017

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The objective of this study was to develop and compare the performance of laboratory grade and portable attenuated total reflectance infrared (ATR-IR) spectroscopic approaches in combination with partial least squares regression (PLSR) for the rapid quantification of alpaca serum IgG concentration, and the identification of low IgG (<1000 mg/dL), which is consistent with the diagnosis of failure of transfer of passive immunity (FTPI) in neonates. Serum samples (n = 175) collected from privately owned, healthy alpacas were tested by the reference method of radial immunodiffusion (RID) assay, and laboratory grade and portable ATR-IR spectrometers. Various pre-processing strategies were applied to the ATR-IR spectra that were linked to corresponding RID-IgG concentrations, and then randomly split into two sets: calibration (training) and test sets. PLSR was applied to the calibration set and calibration models were developed, and the test set was used to assess the accuracy of the analytical method. For the test set, the Pearson correlation coefficients between the IgG measured by RID and predicted by both laboratory grade and portable ATR-IR spectrometers was 0.91. The average differences between reference serum IgG concentrations and the two IR-based methods were 120.5 mg/dL and 71 mg/dL for the laboratory and portable ATR-IR-based assays, respectively. Adopting an IgG concentration <1000 mg/dL as the cut-point for FTPI cases, the sensitivity, specificity, and accuracy for identifying serum samples below this cut point by laboratory ATR-IR assay were 86, 100 and 98%, respectively (within the entire data set). Corresponding values for the portable ATR-IR assay were 95, 99 and 99%, respectively. These results suggest that the two different ATR-IR assays performed similarly for rapid qualitative evaluation of alpaca serum IgG and for diagnosis of IgG <1000 mg/dL, the portable ATR-IR spectrometer performed slightly better, and provides more flexibility for potential application in the field.

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Measurement of serum immunoglobulin G in dairy cattle using Fourier-transform infrared spectroscopy: A reagent free approach

Cited by 24 publications

References 36 publications

Quantification of bovine immunoglobulin G using transmission and attenuated total reflectance infrared spectroscopy

Quantification of bovine immunoglobulin G using transmission and attenuated total reflectance infrared spectroscopy

At‐line monitoring of industrial frying processes using ATR‐FTIR‐PLS method

Application of laboratory and portable attenuated total reflectance infrared spectroscopic approaches for rapid quantification of alpaca serum immunoglobulin G

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