Measurement of the association of cholephylic organic anions with different binding proteins

Gentile, S; Bajema, B.L.; Baldini, Giulia; Lunazzi, Gian Carlo; Groothuis, Genie M.M.; Tiribelli, Claudio; Meijer, Dirk K. F.; Sottocasa, Gian Luigi

doi:10.1016/0006-2952(85)90523-4

Cited by 23 publications

(21 citation statements)

References 9 publications

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“…In this method, unbound and HSA-bound BSP were separated by the use of Blue Sepharose. The Kd values (between 1.4 and 1.9 µM) obtained with the Blue Sepharose method were very similar to the values reported in the literature, [23][24][25] indicating the validity of this method.…”

Section: Discussionsupporting

confidence: 76%

“…[23][24][25] In the present study, we described a new technique for the determination of unbound BSP concentration in the presence of HSA. In this method, unbound and HSA-bound BSP were separated by the use of Blue Sepharose.…”

Section: Discussionmentioning

confidence: 99%

“…The unbound BSP concentration in the presence of albumin has been measured with different techniques, including spectrophotometry, 23 ultrafiltration, 24 and dialysis. 25 Because most measurements were performed with bovine serum albumin and under buffer conditions other than the uptake buffer used in this study, we measured the unbound BSP concentration with a new approach.…”

Section: Determination Of Unbound Bsp Concentration In a Bsp/hsa Mixturementioning

confidence: 99%

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Influence of albumin binding on the substrate transport mediated by human hepatocyte transporters OATP2 and OATP8

Cui

Walter

2003

Journal of Gastroenterology

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Determination Of Unbound Bsp Concentration In a Bsp/hsa Mixturementioning

confidence: 99%

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Influence of albumin binding on the substrate transport mediated by human hepatocyte transporters OATP2 and OATP8

Cui

Walter

2003

Journal of Gastroenterology

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“…The protein in the presence of DCA binds up to 15 tool BSP/mol protein with a KD of 5 ~tmol/1 (Tiribelli et al 1976). Native bilitranslocase in the absence of DCA showed a lower KD of 1.2 gM and bound BSP at a ratio of 3 mol per mole of protein (Gentile et al 1985b). The protein has been reconstituted into artificial liposomes and in erythrocyte membranes ), both of which transported BSP.…”

Section: Carrier Proteins For Hepatic Uptake Of Cholephilic Organic Amentioning

confidence: 99%

Transport of organic anions in the liver. An update on bile acid, fatty acid, monocarboxylate, anionic amino acid, cholephilic organic anion, and anionic drug transport

Petzinger¹

1994

Reviews of Physiology, Biochemistry and Pharmacology

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“…It is reversibly bound to a number of proteins, including albumin and lipoprotein [4]. With a dissociation constant (Kd) of 3 pmol It', as found for in uitro binding to bovine albumin [5], more than 99% of ICG will be protein bound in a plasma sample with 1 pmol 1-I of ICG and the physiological albumin concentration of 400-660 pmol I-'.…”

Section: Introductionmentioning

confidence: 97%

Intrinsic hepatic clearance of indocyanine green in the pig: Dependence on plasma protein concentration

Ott

Keiding

Bass

1992

Eur J Clin Investigation

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Intrinsic hepatic clearance (K) of indocyanine green (ICG) is used as a quantitative measure of liver function. ICG is tightly bound to plasma proteins. The purpose of this study was to examine the effect of changes of plasma protein concentration on K in anaesthetized pigs with intact hepatic circulation. In addition, an attempt was made to evaluate the corresponding changes of the unbound intrinsic clearance of ICG. The plasma protein concentration was changed by exchange of plasma with either dextran-70 or donor pig plasma. Plasma albumin concentration was measured in a peripheral artery and changes of the concentrations of other plasma proteins were assumed to parallel those of albumin. ICG was given as a constant infusion and K was calculated from peripheral artery and hepatic vein concentrations of ICG according to the sinusoidal perfusion model. One experimental series comprised 3 measurement periods: From Period 1 to Period 2 (eight animals) albumin concentration was decreased by 36.6 +/- 6.5% (Mean +/- SD). This was associated with an increase of K of 32.8 +/- 28.8% (P = 0.004). From Period 2 to 3 (five animals) albumin was increased by 13.2 +/- 3.2% and K decreased by 18.5 +/- 8.3% (P = 0.03). In the second experimental series (eight animals), albumin concentration was increased by 21.6 +/- 10.3% and K decreased by 20.3 +/- 8.1% (P = 0.001). For both series, changes in albumin concentration were associated with oppositely directed changes of K in 20 out of 21 comparisons (P less than 0.001). Thus K depends not only on hepatocyte function but also on plasma protein concentration. This finding should affect interpretation of K when used as a liver function test. Changes of the unbound intrinsic clearance of ICG were examined indirectly by means of the K.a product (a: albumin concentration). According to the overall evaluation of the data the unbound intrinsic clearance of ICG was not affected by the changes in plasma protein concentration, but the results were internally inconsistent, apparently due to a time-dependency of the K.a product. We suggest this to be due to a slow but steady decrease of the 'background' K. After correction for the average decrease of K of 0.102% per min our data were in accordance with the hypothesis that the unbound clearance of K was enhanced by the binding protein(s) of ICG.

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Measurement of the association of cholephylic organic anions with different binding proteins

Cited by 23 publications

References 9 publications

Influence of albumin binding on the substrate transport mediated by human hepatocyte transporters OATP2 and OATP8

Influence of albumin binding on the substrate transport mediated by human hepatocyte transporters OATP2 and OATP8

Transport of organic anions in the liver. An update on bile acid, fatty acid, monocarboxylate, anionic amino acid, cholephilic organic anion, and anionic drug transport

Intrinsic hepatic clearance of indocyanine green in the pig: Dependence on plasma protein concentration

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