Measurement of the penetration depths of red and near infrared light in human “ex vivo” tissues

Stolik, S.; Delgado, José A.; Olivares‐Pérez, A.; Anasagasti, L.

doi:10.1016/s1011-1344(00)00082-8

Cited by 398 publications

(321 citation statements)

References 17 publications

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“…48 Despite these considerations, it is known that light penetration into the tissue is proportional to its wavelength when considering the UV-near infrared range, i.e., the longer the wavelength, the deeper is the light penetration. [49][50][51][52] The shorter wavelengths are better absorbed by biological molecules, i.e., the greater part of light intensity is absorbed by the superficial tissue layers and as a consequence, lower penetration is observed. Whereas, photons in the red and infrared range are less absorbed by the biological chromophores and a higher light penetration is achieved.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

141

130

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Objective. In vivo studies of antimicrobial PDT in animal models of oral candidosis are scarce and the association of porphyrin and LED light has not been evaluated for in vivo photoinactivation of Candida. In this study the effectiveness of photodynamic therapy (PDT) on the inactivation of Candida albicans in vivo was evaluated. Study design. Seventy-one 6-week-old female Swiss mice were immunosuppressed, provided tetracycline to their drinking water, then orally swabbed with a suspension of C. albicans (10 7 CFU/mL). Four days after oral inoculation, PDT was performed on the dorsum of the tongue after topical administration of Photogem at 400, 500, or 1000 mg/L and followed 30 minutes later by illumination with LED light (305 J/cm 2 ) at 455 or 630 nm (n ϭ 5 each). After swabbing to recover yeast from the tongue, the number of surviving yeast cells was determined (CFU/mL) and analyzed by ANOVA and Holm-Sidak tests (P Ͻ .05). Animals were humanely killed, and the tongues surgically removed and processed for histological evaluation of presence of yeast and inflammatory reaction. Results. PDT resulted in a significant reduction in C. albicans recovered from the tongue (P Ͻ .001) when compared with mice from the positive control group. There was no difference between the concentrations of Photogem and LED light wavelengths used. Histological evaluation of the tongue revealed that PDT causes no significant adverse effects to the local mucosa. Conclusion. PDT promoted significant reduction in the viability of C. albicans biofilm without harming the tongue tissue.

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Section: Discussionmentioning

confidence: 99%

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

141

130

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“…25,26 Working within BWs allows for overcoming the reduced in vivo penetration lengths achievable by visible-emitting LNPs (hundreds of microns). 27 Although the number of infrared-emitting fluorescent nanoprobes working in the BWs is reduced compared to that of visible-emitting NPs, different IR-emitting QDs, C-NPs, and RENPs have been synthesized and studied for deep tissue in vivo imaging purposes. [28][29][30] Among all these IR-emitting systems, neodymium-doped NPs (Nd:NPs) arise as exceptional candidates for in vivo fluorescence imaging due to their unique combination of properties.…”

Section: Introductionmentioning

confidence: 99%

Neodymium-doped nanoparticles for infrared fluorescence bioimaging: The role of the host

Rosal

Pérez‐Delgado

Misiak

et al. 2015

Journal of Applied Physics

113

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The spectroscopic properties of different infrared-emitting neodymium-doped nanoparticles (LaF 3 :Nd 3þ , SrF 2 :Nd 3þ , NaGdF 4 : Nd 3þ , NaYF 4 : Nd 3þ , KYF 4 : Nd 3þ , GdVO 4 : Nd 3þ , and Nd:YAG) have been systematically analyzed. A comparison of the spectral shapes of both emission and absorption spectra is presented, from which the relevant role played by the host matrix is evidenced. The lack of a "universal" optimum system for infrared bioimaging is discussed, as the specific bioimaging application and the experimental setup for infrared imaging determine the neodymiumdoped nanoparticle to be preferentially used in each case. V C 2015 AIP Publishing LLC.

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“…One of the major drawbacks is that the drugs are excited by visible light which cannot penetrate deeply into tissues. [9][10][11][12][13] Furthermore, the majority of commercial 30 photosensitisers show little discrimination in uptake in diseased cells vs. normal healthy tissue. 14 Both of these limitations may be reduced by exciting the photosensitiser via simultaneous two-photon absorption (TPA).…”

Section: Introductionmentioning

confidence: 99%

One- and two-photon activated phototoxicity of conjugated porphyrin dimers with high two-photon absorption cross sections

et al. 2009

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Measurement of the penetration depths of red and near infrared light in human “ex vivo” tissues

Cited by 398 publications

References 17 publications

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Neodymium-doped nanoparticles for infrared fluorescence bioimaging: The role of the host

One- and two-photon activated phototoxicity of conjugated porphyrin dimers with high two-photon absorption cross sections

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