Measurement of viscoelastic properties of root cell walls affected by low pH in lateral roots of Pisum sativum L.

Tanimoto, Eiichi; Fujii, Shuhei; Yamamoto, Ryōichi; Inanaga, Shinobu

doi:10.1007/978-94-017-2858-4_3

Cited by 17 publications

(38 citation statements)

References 28 publications

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“…The weight was removed after 30-min loading, and then the distance between two dots was measured after the strip had been left for 5 min (35 min after starting). The strain after 35 min was shown as a plastic extension, and the difference between the strain after 30 min and that after 35 min was shown as an elastic extension (Tanimoto et al, 2000).…”

Section: Creep Extension Analysismentioning

confidence: 96%

“…Creep extension has been performed to determine the yield threshold load of cell walls (Kutschera and Kende, 1988;Nakahori et al, 1991;Okamoto and Okamoto, 1994;Pritchard et al, 1990;Tomos and Pritchard, 1994). Moreover, the physical parameters and cell-wall extensibility under low pH have been investigated to clarify rheological processes in the elongation of cell walls (Tanimoto et al, 2000). It follows that studies on petal cell-wall extensibility during rose flower opening are warranted.…”

Section: Introductionmentioning

confidence: 99%

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Cell Wall Extensibility and Effect of Cell-Wall-Loosening Proteins during Rose Flower Opening

Yamada

Takahashi

Fujitani

et al. 2009

J. Japan. Soc. Hort. Sci.

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Section: Creep Extension Analysismentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Cell Wall Extensibility and Effect of Cell-Wall-Loosening Proteins during Rose Flower Opening

Yamada

Takahashi

Fujitani

et al. 2009

J. Japan. Soc. Hort. Sci.

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“…Diameters of the root samples were measured with a low-power microscope and used to determine cross-sectional areas. Individual root samples were secured between two clamps of a Rheoner creep meter (Yamaden RE-33005) so that the extension capacity of root sections at 3 to 6 or 6 to 9 mm from the tip could be assayed (Tanimoto et al, 2000;Hattori et al, 2003). A new root specimen was used for every measurement.…”

Section: Comparative Mechanical Extensibility Of Cell Wallsmentioning

confidence: 99%

Progressive Inhibition by Water Deficit of Cell Wall Extensibility and Growth along the Elongation Zone of Maize Roots Is Related to Increased Lignin Metabolism and Progressive Stelar Accumulation of Wall Phenolics

et al. 2005

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Water deficit caused by addition of polyethylene glycol 6000 at 20.5 MPa water potential to well-aerated nutrient solution for 48 h inhibited the elongation of maize (Zea mays) seedling primary roots. Segmental growth rates in the root elongation zone were maintained 0 to 3 mm behind the tip, but in comparison with well-watered control roots, progressive growth inhibition was initiated by water deficit as expanding cells crossed the region 3 to 9 mm behind the tip. The mechanical extensibility of the cell walls was also progressively inhibited. We investigated the possible involvement in root growth inhibition by water deficit of alterations in metabolism and accumulation of wall-linked phenolic substances. Water deficit increased expression in the root elongation zone of transcripts of two genes involved in lignin biosynthesis, cinnamoyl-CoA reductase 1 and 2, after only 1 h, i.e. before decreases in wall extensibility. Further increases in transcript expression and increased lignin staining were detected after 48 h. Progressive stress-induced increases in wall-linked phenolics at 3 to 6 and 6 to 9 mm behind the root tip were detected by comparing Fourier transform infrared spectra and UV-fluorescence images of isolated cell walls from water deficit and control roots. Increased UV fluorescence and lignin staining colocated to vascular tissues in the stele. Longitudinal bisection of the elongation zone resulted in inward curvature, suggesting that inner, stelar tissues were also rate limiting for root growth. We suggest that spatially localized changes in wall-phenolic metabolism are involved in the progressive inhibition of wall extensibility and root growth and may facilitate root acclimation to drying environments.

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“…For example, according to the acid growth hypothesis of Rayle and Cleland (1992), maintenance of sufficiently acid pH in the cell wall facilitates turgor-driven expansion by directly loosening the polysaccharide matrix or by optimizing the activity of cell wall proteins and enzymes associated with loosening (Cosgrove, 1997;Tanimoto et al, 2000;Wu and Cosgrove, 2000). Recent findings that cell wall acidification is associated with auxin effects on seed embryo growth (Rober-Kleber et al, 2003) and with early growth responses to gravistimulation of root cells (Taylor et al, 1996;Fasano et al, 2001) support the existence of a close relationship between wall acidification and cell expansion.…”

mentioning

confidence: 99%

The Spatially Variable Inhibition by Water Deficit of Maize Root Growth Correlates with Altered Profiles of Proton Flux and Cell Wall pH

Fan

Neumann

2004

Plant Physiology

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Growth of elongating primary roots of maize (Zea mays) seedlings was approximately 50% inhibited after 48 h in aerated nutrient solution under water deficit induced by polyethylene glycol 6000 at 20.5 MPa water potential. Proton flux along the root elongation zone was assayed by high resolution analyses of images of acid diffusion around roots contacted for 5 min with pH indicator gel. Profiles of root segmental elongation correlated qualitatively and quantitatively (r 2 5 0.74) with proton flux along the surface of the elongation zone from water-deficit and control treatments. Proton flux and segmental elongation in roots under water deficit were remarkably well maintained in the region 0 to 3 mm behind the root tip and were inhibited from 3 to 10 mm behind the tip. Associated changes in apoplastic pH inside epidermal cell walls were measured in three defined regions along the root elongation zone by confocal laser scanning microscopy using a ratiometric method. Finally, external acidification of roots was shown to specifically induce a partial reversal of growth inhibition by water deficit in the central region of the elongation zone. These new findings, plus evidence in the literature concerning increases induced by acid pH in wall-extensibility parameters, lead us to propose that the apparently adaptive maintenance of growth 0 to 3 mm behind the tip in maize primary roots under water deficit and the associated inhibition of growth further behind the tip are related to spatially variable changes in proton pumping into expanding cell walls.

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Measurement of viscoelastic properties of root cell walls affected by low pH in lateral roots of Pisum sativum L.

Cited by 17 publications

References 28 publications

Cell Wall Extensibility and Effect of Cell-Wall-Loosening Proteins during Rose Flower Opening

Cell Wall Extensibility and Effect of Cell-Wall-Loosening Proteins during Rose Flower Opening

Progressive Inhibition by Water Deficit of Cell Wall Extensibility and Growth along the Elongation Zone of Maize Roots Is Related to Increased Lignin Metabolism and Progressive Stelar Accumulation of Wall Phenolics

The Spatially Variable Inhibition by Water Deficit of Maize Root Growth Correlates with Altered Profiles of Proton Flux and Cell Wall pH

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