Measurement of vitamin K in human liver by gradient elution high-performance liquid chromatography using platinum-black catalyst reduction and fluorimetric detection

Usui, Yuji; Nishimura, N; Kobayashi, Nobuaki; Okanoue, Toyotake; Kimoto, Michio; Ozawa, Kazue

doi:10.1016/s0378-4347(00)82907-2

Cited by 47 publications

(15 citation statements)

References 21 publications

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“…Samples were centrifuged and plasma was stored at -20°C before being further analyzed. Plasma concentrations of ·-tocopherol, phylloquinone and retinol were simultaneously measured using a reversed-phase, high-pressure liquid chromatography method in plasma by platinum-black catalyst reduction and fluorometric detection, eluent methanol 100%, flow rate 0.8 ml/min [12,13]. Determination of the plasma concentrations was done by linear regression from a calibration curve.…”

Section: Methodsmentioning

confidence: 99%

Fat-Soluble Vitamins in Patients with Acute Renal Failure

Druml

Schwarzenhofer

Apsner

et al. 1998

Mineral Electrolyte Metab

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Objective: Systematic investigations on the status of fat-soluble vitamins in patients with acute renal failure (ARF) are lacking and hence no recommendations for vitamin supply can be defined for these subjects. Thus we compared the status of fat-soluble vitamins, of transport molecules and some vitamin-dependent proteins in patients with ARF and healthy controls. Setting: Nephrology unit of a university hospital. Patients and Methods: Eight patients with ARF requiring hemodialysis therapy were investigated and 28 healthy volunteers served as controls. Plasma concentrations of retinol (vitamin A) and retinol-binding protein (RBP), 25-OH and 1,25-(OH)₂ vitamin D₃, of parathyroid hormone (PTH), of α-tocopherol (vitamin E) and of phylloquinone (vitamin K), osteocalcin and noncarboxylated osteocalcin, respectively, were measured and plasma lipoprotein fractions (as vitamin transport vehicle) were evaluated. Results: Vitamin A levels were decreased (p < 0.001), but RBP levels were normal in ARF patients. Vitamin D₃ metabolites 25-OH and 1,25-(OH)₂ vitamin D₃ plasma levels were profoundly depressed, and PTH was elevated (p < 0.001). Vitamin E plasma concentration was reduced (p < 0.001) but this cannot be accounted for by decreased LDL cholesterol or triglyceride levels. In contrast, vitamin K plasma level was rather elevated in ARF patients with a broad range of individual values. Blood coagulation was normal but total and carboxylated osteocalcin were decreased. No correlation of vitamin K concentrations and any of the plasma lipoprotein fractions could be identified. Conclusion: With the exception of vitamin K, profound deficiencies of fat-soluble vitamins develop in patients with ARF. Current recommendations for vitamin supplementation are inadequate and should be re-evaluated for these patients.

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Section: Methodsmentioning

confidence: 99%

Fat-Soluble Vitamins in Patients with Acute Renal Failure

Druml

Schwarzenhofer

Apsner

et al. 1998

Mineral Electrolyte Metab

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“…In addition, MK-4 has received governmental approval for use as an agent for the treatment of osteoporosis in Japan. Thus, measurement of MK-4 Previously, several methods for separation and determination of Vitamin K by thin layer chromatography (TLC) [17], gas liquid chromatography (GLC) [18,19] and highperformance liquid chromatography (HPLC) with ultraviolet (UV) detection [20], fluorescence detection [21][22][23][24][25], electrochemical detection [26][27][28][29] and mass spectrometric detection [30] were reported. Recently, we have developed a method for the determination of Vitamin K in human plasma by liquid chromatograpy-atmospheric pressure chemical ionization/mass spectrometry (LC-APCI/MS) [31].…”

Section: Introductionmentioning

confidence: 99%

Determination of plasma Vitamin K by high-performance liquid chromatography with fluorescence detection using Vitamin K analogs as internal standards

Kamao

Suhara

Tsugawa

et al. 2005

Journal of Chromatography B

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“…Usui et al 28) reported vitamin K contents in the noncancerous portion of 38 human liver specimens from patients with primary or metastatic liver cancer. They included 6 normal liver tissues and 22 cirrhotic liver tissues.…”

Section: Discussionmentioning

confidence: 99%

Vitamin K Contents in Liver Tissue of Hepatocellular Carcinoma Patients

Miyakawa

Kajiwara²,

Shirahata³

et al. 2000

Japanese Journal of Cancer Research

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Serum protein induced in vitamin K absence-II (PIVKA-II) is used as a tumor marker because it increases at a notably higher rate in patients with hepatocellular carcinoma. To clarify the mechanism causing the elevation of serum PIVKA-II, we measured the contents of vitamins K 1 (phylloquinone, PK) and K 2 (menaquinone, MK) (MK-4, MK-5, MK-6, MK-7, MK-8, MK-9, MK-10) in liver tissue resected from 21 hepatic cancer patients (12 patients with hepatocellular carcinoma and 9 patients with metastatic hepatic cancer), using HPLC combined with coulometric reduction and fluorometric detection. In the cancerous tissue of hepatocellular carcinoma patients, PK, MK-7, MK-8, and MK-10 were significantly lower than that found in the noncancerous tissue. Furthermore, MK-6, MK-7, MK-8, and MK-10 in the cancerous tissue of hepatocellular carcinoma patients were significantly lower than that in the cancerous tissue of metastatic hepatic cancer patients. These data suggested that one of the mechanisms of the elevation of serum PIVKA-II levels in hepatocellular carcinoma patients is a vitamin K deficiency in the local cancerous tissue.

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Measurement of vitamin K in human liver by gradient elution high-performance liquid chromatography using platinum-black catalyst reduction and fluorimetric detection

Cited by 47 publications

References 21 publications

Fat-Soluble Vitamins in Patients with Acute Renal Failure

Fat-Soluble Vitamins in Patients with Acute Renal Failure

Determination of plasma Vitamin K by high-performance liquid chromatography with fluorescence detection using Vitamin K analogs as internal standards

Vitamin K Contents in Liver Tissue of Hepatocellular Carcinoma Patients

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