Vitamin K deficiency has been reported in patients who were treated with antibiotics and placed on poor diets after surgery. High-performance liquid chromatography (HPLC) was used to study the influence of dietary intake on vitamin K concentrations in surgical patients (n = 22). Plasma phylloquinone decreased rapidly from 1.19 +/- 0.16 to 0.47 +/- 0.12 nmol/L (means +/- SEM, n = 11) on a low-phylloquinone diet and from 1.16 +/- 0.12 to 0.36 +/- 0.07 nmol/L (n = 11) by postoperative fasting. A small amount of phylloquinone and a large amount of menaquinone were found in liver tissue. Phylloquinone concentration was 28.0 +/- 4.3 pmol/g liver (wet weight) on the standard diet (n = 7) whereas it was 6.8 +/- 1.1 pmol/g on the low-phylloquinone diet after 3 d (n = 8). Because phylloquinone is rapidly depleted by fasting, it may be difficult to prevent vitamin K deficiency by dietary phylloquinone alone during long-term fasting after surgery.

Pigs weighing approximately 70 kg were used in two experiments to determine the valine requirement during the finishing period. In the first experiment, 10 gilts were allotted in two 5 x 5 Latin square designs to five semipurified diets that ranged in valine concentration from .35 to .55%. Urinary urea excretion was measured during each of the 3-d periods of the Latin square. Urea N excretion in relation to N intake and to creatinine N excretion was minimized (quadratic effect, P < .10) at valine concentrations of .45 to .50%. In Exp. 2, 36 barrows and 36 gilts were fed one of six diets containing .35 to .60% valine. The highest weight gains (not significant) and feed efficiencies (quadratic effect, P < .05) were achieved by the pigs that consumed .45% valine. Plasma urea concentration at the end of the experiment was lowest (quadratic effect, P < .05) in pigs that consumed .50% valine. Estimates of the valine requirement based on breakpoint and quadratic equation analyses ranged from .40 to .50% total valine (.33 to .43% ileal digestible valine). Pigs in Exp. 2 consumed approximately 2.5 kg/d (8,850 kcal/d of ME). Thus, the estimate of the valine requirement is approximately 11 g/d. These estimates of the valine requirements of finishing pigs are slightly higher than the National Research Council requirements when expressed as a percentage of the diet but are similar when expressed on a grams per day basis.

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1. The metabolism of 3,5,3',5'-tetrachlorobiphenyl (TCB) was investigated with liver microsomes and purified P450 from the male Wistar rat. 2. One novel metabolite was produced after incubation with liver microsomes derived from the 3-methylcholanthrene (MC)- and 3,4,5,3',4'-pentachlorobiphenyl-pretreated rat, but not after incubation with those from the untreated or phenobarbital (PB)-pretreated rat. These results suggest that P450 isozyme(s) induced by MC-type inducers is involved in 3,5,3',5'-TCB metabolism. 3. The chemical structure of this metabolite was identified to be 4-hydroxy-3,5,3',5'-TCB by comparison of its retention time in glc and the ms with those of a synthetic sample. 4. Purified rat P4501A1, a major MC-inducible P450 isozyme, catalyzed the 4-hydroxylation of 3,5,3',5'-TCB, but P4502B1, a major PB-inducible isozyme, was inactive. 5. Reduced glutathione completely inhibited the formation of the hydroxylated metabolite, suggesting that 4-hydroxylation of 3,5,3',5'-TCB proceeded via the 3,4-epoxide.

Studies were made on changes in the level of plasma immunoreactive glucagon (IRG) in rats with lesions of the ventromedial hypothalamic area. In the rats with hypothalamic lesions (HTL) the basal level of plasma immunoreactive insulin was higher and its responses to intravenous injection of glucose and to intraperitoneal administration of arginine were significantly greater than those in control rats. The basal plasma IRG concentration was lower and its changes in response to 24 hr starvation or the arginine load were significantly less in rats with HTL than in controls at 1 and 10 weeks after the operation. Similar results were also obtained in the rats pair-fed for 4 weeks after the operation. A significant negative correlation was observed between the basal plasma IRG level and body weight or Lee's index in these rats. These data suggest that disturbance of the autonomic nervous system due to destruction of the hypothalamus, reduced secretion of pituitary hormones, increased insulin secretion and defect in humoral factors of the hypothalamus may be responsible for the decrease in plasma IRG levels observed in rats with HTL. It also seems likely that reduced glucagon secretion contributes at least in one part to the development of hypothalamic obesity.