Measurement uncertainty evaluation of cellular spheroids surface tension in compressing tests using Young-Laplace equation

Beatrici, Anderson; Baptista, Leandra Santos; Granjeiro, José Mauro

doi:10.1088/1742-6596/975/1/012027

Cited by 2 publications

(3 citation statements)

References 5 publications

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“…The combined uncertainties in force depend on the force modulus, so the relative uncertainties in force are around 2%. This condition is just reached by quasi‐spherical samples and considering the isotropic mechanical resistance of spheroids to Young's modulus and surface tension, both the expanded uncertainties remain about 10% 36 …”

Section: Methodsmentioning

confidence: 95%

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The hypertrophic cartilage induction influences the building‐block capacity of human adipose stem/stromal cell spheroids for biofabrication

et al. 2021

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As an alternative to the classical tissue engineering approach, bottom-up tissue engineering emerges using building blocks in bioassembly technologies. Spheroids can be used as building blocks to reach a highly complex ordered tissue by their fusion (bioassembly), representing the foundation of biofabrication. In this study, we analyzed the biomechanical properties and the fusion capacity of human adipose stem/stromal cell (ASC) we spheroids during an in vitro model of hypertrophic cartilage established by our research group. Hypertrophic induced-ASC spheroids showed a statistically significant higher Young's modulus at weeks 2 (P < .001) and 3 (P < .0005) compared with non-induced. After fusion, non-induced and induced-ASC spheroids increased the contact area and decreased their pairs' total length. At weeks 3 and 5, induced-ASC spheroids did not fuse completely, and the cells migrate preferentially in the fusion contact region. Alizarin red O staining showed the highest intensity of staining in the fused induced-ASC spheroids at week 5, together with intense staining for collagen type I and osteocalcin. Transmission electron microscopy and element content analysis (X-ray Energy Dispersive Spectroscopy) revealed in the fused quartet at week 3 a crystal-like structure. Hypertrophic induction interferes with the intrinsic capacity of spheroids to fuse. The measurements of contact between spheroids during the fusion process, together with the change in viscoelastic | 1209 Kronemberger et al. How to cite this article: Kronemberger GS, Beatrici A, Dalmônico GML, et al. The hypertrophic cartilage induction influences the building-block capacity of human adipose stem/stromal cell spheroids for biofabrication.

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Section: Methodsmentioning

confidence: 95%

“…This condition is just reached by quasi-spherical samples and considering the isotropic mechanical resistance of spheroids to Young's modulus and surface tension, both the expanded uncertainties remain about 10%. 36…”

Section: Biomechanical Assaymentioning

confidence: 99%

The hypertrophic cartilage induction influences the building‐block capacity of human adipose stem/stromal cell spheroids for biofabrication

et al. 2021

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“…Analyzing relaxation force and shape relaxation after squeezing spheroid between parallel plates. [20,25,[63][64][65]78,79,176] Probing from within…”

Section: Surface Tension and Viscositymentioning

confidence: 99%

Spheroid mechanics and implications for cell invasion

Boot

Koenderink

Boukany

2021

Advances in Physics: X

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Spheroids are widely used in vitro 3D multicellular model systems that mimic complex physiological microenvironments of tissues. As different cell types vary in deformability and adhesion, the choice of (heterogeneous) cell composition will define overall spheroid mechanics, including their viscoelasticity and effective surface tension. These mechanical parameters directly influence cell sorting and possibly cell invasion into the extracellular matrix. Spheroid models therefore provide fundamental insights in the relation between cellular mechanics and important physiological processes, such as tissue formation, embryonic tissue remodeling, and cancer metastasis. In this review, we first summarize and compare current biophysical tools that probe mechanics of spheroids either from the outside or from within, then relate spheroid mechanics to cell mechanics and cell-cell adhesion, and subsequently discuss the role of spheroid mechanics alongside surrounding microenvironment parameters in (cancer) cell migration. We conclude by pointing out the research gaps and drawing the attention to novel techniques that could shed more light on the biophysical characterization of spheroids in the framework of tissue remodeling and cancer metastasis.

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Measurement uncertainty evaluation of cellular spheroids surface tension in compressing tests using Young-Laplace equation

Cited by 2 publications

References 5 publications

The hypertrophic cartilage induction influences the building‐block capacity of human adipose stem/stromal cell spheroids for biofabrication

The hypertrophic cartilage induction influences the building‐block capacity of human adipose stem/stromal cell spheroids for biofabrication

Spheroid mechanics and implications for cell invasion

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