Measurements of Interleukin‐6 in Gingival Crevicular Fluid From Adults With Destructive Periodontal Disease

Abstract

This paper describes a study of whether or not the amounts of interleukin‐6 (IL‐6) in gingival crevicular fluid (GCF) are correlated with periodontal clinical measures. A sensitive ELISA was developed to measure IL‐6 in GCF. Two male and 3 female adult subjects with periodontal disease were examined at their first appointments, after 3 months, and after 6 months. Data were obtained on plaque index (PI), bleeding index (BI), probing depth (PD), and on the IL‐6 content of GCF samples from 16 sites per subject for a total of 240 measurements. Significant correlations were found between BI and IL‐6 (P < 0.005) and between PD and IL‐6 (P <0.05), but not between PI and IL6. Only 6 out of the 80 sites (in 3 of the 5 subjects) showed PD increases of at least 2 mm. However, for each of these 3 subjects, the amounts of IL‐6 in the GCF samples from these sites were markedly higher than the mean amounts of IL‐6 in the GCF samples from the remaining sites. These findings suggest that IL‐6 may be a useful indicator of periodontal disease, although more extensive longitudinal studies are needed to determine the real clinical value of this GCF component. J Periodontol 1993; 64:980–983.

Porphyromonas gingivalis Induction of MicroRNA-203 Expression Controls Suppressor of Cytokine Signaling 3 in Gingival Epithelial Cells

“…Reduction of SOCS levels also increased the activation status of Stat3, a transcription factor that binds to IL-6-responsive promoter elements (27). P. gingivalis can stimulate the production of IL-6 from gingival epithelial cells (47), and IL-6 levels are elevated in gingival crevicular fluid in periodontal disease (14,26). Hence, induction of miR-203 by P. gingivalis has the potential to contribute to the inflammation associated with periodontitis.…”

Arginine-Specific Protease fromPorphyromonas gingivalisActivates Protease-Activated Receptors on Human Oral Epithelial Cells and Induces Interleukin-6 Secretion

“…Compared to normal tissue, IL-6 expression appears to be elevated in inflamed tissue from periodontitis sites and is highest in gingivitis sites (33,59). Studies have indicated that IL-6 levels are higher in GCF from refractory periodontitis patients and may also correlate with progression of disease in these patients (13,29,45). IL-6 stimulates plasma cell proliferation and therefore may promote the presence of plasma cells that are readily found in periodontitis lesions (47).…”

Increased IL-6 Levels in Peri-Implant Crevicular Fluid Correlate with Peri-Implantitis

“…High amounts of IL-6 were detected in patients with periodontitis (21,22), in active sites of refractory periodontitis (23), as well as in sites undergoing orthodontic movement, emphasizing the important role of IL-6 in the bone remodeling process (24). IL-6 was observed to correlate with the severity of periodontal disease (25), which may indicate that IL-6 could be a diagnostic marker for sites of active periodontal disease (26).…”