Measurements of liposome biomechanical properties by combining line optical tweezers and dielectrophoresis

Spyratou, Ellas; Cunaj, Efrosini; Tsigaridas, G.; Mourelatou, Elena A.; Demetzos, Costas; Serafetinides, A. A.; Makropoulou, M.

doi:10.3109/08982104.2014.987784

Cited by 12 publications

(6 citation statements)

References 54 publications

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“…This value is appreciably higher than that, κ ≅ 25 k B T , often used in the theoretical literature . The conventionally measured values of κ are, however, reported to be in a wide range (see, e.g., refs , , and references therein) and the maximal values, ∼600 k B T , are comparable with our estimate. In fact, the maximum value, measured in J, was 10 –19 J (ref ).…”

Section: Resultssupporting

confidence: 77%

Quantitative Profiling of Nanoscale Liposome Deformation by a Localized Surface Plasmon Resonance Sensor

et al. 2016

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Characterizing the shape of sub-100 nm, biological soft-matter particulates (e.g., liposomes and exosomes) adsorbed at a solid-liquid interface remains a challenging task. Here, we introduce a localized surface plasmon resonance (LSPR) sensing approach to quantitatively profile the deformation of nanoscale, fluid-phase 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes contacting a titanium dioxide substrate. Experimental and theoretical results validate that, due to its high sensitivity to the spatial proximity of phospholipid molecules near the sensor surface, the LSPR sensor can discriminate fine differences in the extent of ionic strength-modulated liposome deformation at both low and high surface coverages. By contrast, quartz crystal microbalance-dissipation (QCM-D) measurements performed with equivalent samples were qualitatively sensitive to liposome deformation only at saturation coverage. Control experiments with stiffer, gel-phase 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes verified that the LSPR measurement discrimination arises from the extent of liposome deformation, while the QCM-D measurements yield a more complex response that is also sensitive to the motion of adsorbed liposomes and coupled solvent along with lateral interactions between liposomes. Collectively, our findings demonstrate the unique measurement capabilities of LSPR sensors in the area of biological surface science, including competitive advantages for probing the shape properties of adsorbed, nanoscale biological particulates.

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Section: Resultssupporting

confidence: 77%

Quantitative Profiling of Nanoscale Liposome Deformation by a Localized Surface Plasmon Resonance Sensor

et al. 2016

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“…This relatively young technique provides the non‐mechanical manipulation of biological particles such as viruses, living cells, and subcellular organelles. OTs are now being used in the investigation of an increasing number of biochemical and biophysical processes, from the mechanical properties of biological polymers to the multitude of molecular machines that drive the internal dynamics of the cell (Galla et al, ; Khatibzadeh et al, ; Pesce et al, ; Spyratou et al, ). OTs are compatible with fluorescence‐imaging techniques.…”

Section: Multimodal Contrast Mechanismsmentioning

confidence: 99%

Multimodal and non‐linear optical microscopy applications in reproductive biology

Adur

Barbosa

Baratti

et al. 2016

Microscopy Res & Technique

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A plethora of optical techniques is currently available to obtain non-destructive, contactless, real time information with subcellular spatial resolution to observe cell processes. Each technique has its own unique features for imaging and for obtaining certain biological information. However none of the available techniques can be of universal use. For a comprehensive investigation of biological specimens and events, one needs to use a combination of bioimaging methods, often at the same time. Some modern confocal/multiphoton microscopes provide simultaneous fluorescence, fluorescence lifetime imaging, and four-dimensional imaging. Some of them can also easily be adapted for harmonic generation imaging, and to permit cell manipulation technique. In this work we present a multimodal optical workstation that extends a commercially available confocal microscope to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools. The nonlinear microscopy capabilities were added to the commercial confocal microscope by exploiting all the flexibility offered by the manufacturer. The various capabilities of this workstation as applied directly to reproductive biology are discussed. Microsc. Res. Tech. 79:567-582, 2016. © 2016 Wiley Periodicals, Inc.

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“…Subsequently, the technique has grown into a major investigation tool giving it a multidisciplinary nature. For example, the technique is used to investigate, short-range colloidal interactions, and cellular-liposome interactions [6]. Moreover, the technique is used for manipulating microscopic objects such as rotation of microscopic objects, liposome biomechanics, and single molecule force spectroscopy [4] [5] [15] [7].…”

Section: Introductionmentioning

confidence: 99%

Planar Optical Tweezer Trap (2D-LOT) System Realized by Light Sheet Illumination & Orthogonal Widefield Detection

Baro,

Mondal

2024

Preprint

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We report the realization of the first planar optical tweezer trap system by a sheet of light. To visualize the trapping of the target object (dielectric bead or live cell) in a plane, an orthogonal widefield detection is employed. The planar / two-dimensional lightsheet optical tweezer (2D-LOT) sub-system is realized in an inverted microscopy mode with illumination from the bottom. A 1064 nm laser (power 54.5 W) is expanded and directed to a combination of cylindrical lens and high NA objective lens to generate a tightly-focussed diffraction-limited light sheet. The object to be trapped is injected in the specimen chamber (consists of two coverslips placed at a distance of approx 1 mm) using a syringe. The solution containing the objects stayed in the chamber due to the surface tension of the fluid. The illumination is carried out from Z-direction (with coverslip along XZ-plane) whereas, the detection is achieved perpendicular to the coverslip (along Y-axis). The orthogonal detection is employed to directly visualize the trapping in a plane. The characterization of system PSF estimates the size of light sheet trap PSF to be, 2073.84 μ2which defines the active trap region / area. Beads are tracked on their way to the trap region for determining the trap stiffness along Z and X i.e, kz=1.13 ± 0.034 pN and kx= 0.74 ± 0.021 pN. Results (image and video) show real-time trapping of dielectric beads in the trap zone (2D plane) generated by the light sheet. The beads can be seen getting trapped from all directions in the XZ-plane. Prolonged exposure to the light sheet builds up a 2D array of beads in the trap zone. Similar experiments on live NIH3T3 cells show cells trapped in the 2D trap. The potential of the planar trap lies in its ability to confine objects in two dimensions, thereby opening new kinds of experiments in biophysics, atomic physics, and optical physics.

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Measurements of liposome biomechanical properties by combining line optical tweezers and dielectrophoresis

Cited by 12 publications

References 54 publications

Quantitative Profiling of Nanoscale Liposome Deformation by a Localized Surface Plasmon Resonance Sensor

Quantitative Profiling of Nanoscale Liposome Deformation by a Localized Surface Plasmon Resonance Sensor

Multimodal and non‐linear optical microscopy applications in reproductive biology

Planar Optical Tweezer Trap (2D-LOT) System Realized by Light Sheet Illumination & Orthogonal Widefield Detection

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