Measuring Absolute Membrane Potential Across Space and Time

Lazzari-Dean, Julia R.; Gest, Anneliese; Miller, Evan W.

doi:10.1146/annurev-biophys-062920-063555

Cited by 22 publications

(10 citation statements)

References 124 publications

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“…The potential distribution across membranes and its changes control many biophysical and biochemical processes at and in membranes. ,,− Elucidating these electric properties is yet a challenge and frequently relies upon studies with biomimetic membrane models. ,− Such systems are less complex than the natural template, but the theoretical or experimental treatment is nevertheless a formidable task. This is also true for lipid-tethered bilayer membrane studied in this work.…”

Section: Discussionmentioning

confidence: 99%

Potential Distribution across Model Membranes

et al. 2022

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Membrane models assembled on electrodes are widely used tools to study potential-dependent molecular processes at or in membranes. However, the relationship between the electrode potential and the potential across the membrane is not known. Here we studied lipid bilayers immobilized on mixed self-assembled monolayers (SAM) on Au electrodes. The mixed SAM was composed of thiol derivatives of different chain lengths such that between the islands of the short one, mercaptobenzonitrile (MBN), and the tethered lipid bilayer an aqueous compartment was formed. The nitrile function of MBN, which served as a reporter group for the vibrational Stark effect (VSE), was probed by surface-enhanced infrared absorption spectroscopy to determine the local electric field as a function of the electrode potential for pure MBN, mixed SAM, and the bilayer system. In parallel, we calculated electric fields at the VSE probe by molecular dynamics (MD) simulations for different charge densities on the metal, thereby mimicking electrode potential changes. The agreement with the experiments was very good for the calculations of the pure MBN SAM and only slightly worse for the mixed SAM. The comparison with the experiments also guided the design of the bilayer system in the MD setups, which were selected to calculate the electrode potential dependence of the transmembrane potential, a quantity that is not directly accessible by the experiments. The results agree very well with estimates in previous studies and thus demonstrate that the present combined experimental–theoretical approach is a promising tool for describing potential-dependent processes at biomimetic interfaces.

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Section: Discussionmentioning

confidence: 99%

Potential Distribution across Model Membranes

et al. 2022

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“…In this scenario, Equations (1)–(4) constitute a minimum model based on conductances ultimately related to specific proteins. In principle, the transcription, translation, and post-translational gating of these ion channel and junction proteins are amenable to external modulation and future therapeutic strategies [ 3 , 12 , 13 , 15 , 45 , 56 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. Consequently, the bioelectrical patterns and their encoded information could be externally regulated by acting on multicellular mean field phenomena such as electrical potential, potassium, and calcium waves [ 3 , 4 , 5 , 9 , 72 , 73 ] as a complementary procedure to addressing individual cell characteristics.…”

Section: Discussionmentioning

confidence: 99%

Cell Systems Bioelectricity: How Different Intercellular Gap Junctions Could Regionalize a Multicellular Aggregate

Riol

Cervera

Levin

et al. 2021

Cancers

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Electric potential distributions can act as instructive pre-patterns for development, regeneration, and tumorigenesis in cell systems. The biophysical states influence transcription, proliferation, cell shape, migration, and differentiation through biochemical and biomechanical downstream transduction processes. A major knowledge gap is the origin of spatial patterns in vivo, and their relationship to the ion channels and the electrical synapses known as gap junctions. Understanding this is critical for basic evolutionary developmental biology as well as for regenerative medicine. We computationally show that cells may express connexin proteins with different voltage-gated gap junction conductances as a way to maintain multicellular regions at distinct membrane potentials. We show that increasing the multicellular connectivity via enhanced junction function does not always contribute to the bioelectrical normalization of abnormally depolarized multicellular patches. From a purely electrical junction view, this result suggests that the reduction rather than the increase of specific connexin levels can also be a suitable bioelectrical approach in some cases and time stages. We offer a minimum model that incorporates effective conductances ultimately related to specific ion channel and junction proteins that are amenable to external regulation. We suggest that the bioelectrical patterns and their encoded instructive information can be externally modulated by acting on the mean fields of cell systems, a complementary approach to that of acting on the molecular characteristics of individual cells. We believe that despite the limitations of a biophysically focused model, our approach can offer useful qualitative insights into the collective dynamics of cell system bioelectricity.

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“…The plasma membrane establishes electrochemical potential gradients, referred to as membrane voltage (V mem ), which is critical for a wide range of physiological processes. 358 Traditionally associated with excitable cells for functions like neurotransmitter release and muscle contraction, V mem dynamics has now been recognized to play essential roles in fundamental processes such as cell division, migration, and differentiation, extending beyond excitable cells. Therefore, voltage imaging techniques provide valuable spatial information about the dynamic changes in V mem across the membrane.…”

Section: Membrane Potential and Tensionmentioning

confidence: 99%

Hybrid Small-Molecule/Protein Fluorescent Probes

Minoshima,

Reja,

Hashimoto

et al. 2024

Chem. Rev.

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Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein localization and function in living cells. These hybrid probes are constructed by diverse sitespecific chemical protein labeling approaches through chemical reactions to exogenous peptide/ small protein tags, enzymatic post-translational modifications, bioorthogonal reactions for genetically incorporated unnatural amino acids, and ligand-directed chemical reactions. The hybrid smallmolecule/protein fluorescent probes are employed for imaging protein trafficking, conformational changes, and bioanalytes surrounding proteins. In addition, fluorescent hybrid probes facilitate visualization of protein dynamics at the single-molecule level and the defined structure with superresolution imaging. In this review, we discuss development and the bioimaging applications of fluorescent probes based on small-molecule/protein hybrids.

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Measuring Absolute Membrane Potential Across Space and Time

Cited by 22 publications

References 124 publications

Potential Distribution across Model Membranes

Potential Distribution across Model Membranes

Cell Systems Bioelectricity: How Different Intercellular Gap Junctions Could Regionalize a Multicellular Aggregate

Hybrid Small-Molecule/Protein Fluorescent Probes

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