Measuring conservation of contiguous sets of autosomal markers on bovine and porcine genomes in relation to the map of the human genome

Jiang, Zhihua; Melville, J. S.; Cao, Honghe; Kumar, Sudhir; Filipski, Alan; Gibbins, Ann M. Verrinder

doi:10.1139/g02-038

Cited by 10 publications

(5 citation statements)

References 38 publications

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“…Comparative genomics can be used to investigate complex multigenic biological phenomenon, as in principle, essential mechanisms should be conserved in relatively closely related species. Cattle and humans share 72% sequence homology [1] and higher sequence similarity, on average, than that exhibited between human and mouse (F. S. L. Brinkman, unpublished data). Mouse is often used as the animal model of choice to study infectious diseases and the resulting immune responses in mammals.…”

Section: Introductionmentioning

confidence: 99%

Bovine and human cathelicidin cationic host defense peptides similarly suppress transcriptional responses to bacterial lipopolysaccharide

Mookherjee

Wilson

Doria

et al. 2006

Journal of Leukocyte Biology

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Genomic approaches can be exploited to expose the complexities and conservation of biological systems such as the immune network across various mammalian species. In this study, temporal transcriptional expression profiles were analyzed in human and bovine monocytic cells in response to the TLR-4 agonist, LPS, in the presence or absence of their respective host defense peptides. The cathelicidin peptides, human LL-37 and bovine myeloid antimicrobial peptide-27 (BMAP-27), are homologs, yet they have diverged notably in terms of sequence similarity. In spite of their low sequence similarities, both of these cathelicidin peptides demonstrated potent, antiendotoxin activity in monocytic cells at low, physiologically relevant concentrations. Microarray studies indicated that 10 ng/ml LPS led to the up-regulation of 125 genes in human monocytes, 106 of which were suppressed in the presence of 5 mug/ml of the human peptide LL-37. To confirm and extend these data, temporal transcriptional responses to LPS were assessed in the presence or absence of the species-specific host defense peptides by quantitative real-time PCR. The transcriptional trends of 20 LPS-induced genes were analyzed in bovine and human monocytic cells. These studies demonstrated conserved trends of gene responses in that both peptides were able to profoundly suppress many LPS-induced genes. Consistent with this, the human and bovine peptides suppressed LPS-induced translocation of NF-kappaB subunits p50 and p65 into the nucleus of monocytic cells. However, there were also distinct differences in responses to LPS and the peptides; for example, treatment with 5 mug/ml BMAP-27 alone tended to influence gene expression (RELA, TNF-alpha-induced protein 2, MAPK phosphatase 1/dual specificity phosphatase 1, IkappaBkappaB, NFkappaBIL1, TNF receptor-associated factor 2) to a greater extent than did the same amount of human LL-37. We hypothesize that the immunomodulatory effects of the species-specific host defense peptides play a critical role in regulating inflammation and represent an evolutionarily conserved mechanism for maintaining homeostasis, although the sequence divergence of these peptides is substantial.

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Section: Introductionmentioning

confidence: 99%

Bovine and human cathelicidin cationic host defense peptides similarly suppress transcriptional responses to bacterial lipopolysaccharide

Mookherjee

Wilson

Doria

et al. 2006

Journal of Leukocyte Biology

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“…An inversion of gene order was defined between 80 and 150 Mb of human chromosome 4 (GPIG4 to EDNRA) which corresponds from 70 cM to the end of the linkage group on pig chromosome 8, consistent with previous reports. 1 This breakpoint likely lies between SCARB2 and GPIG4 at 78 and 82 Mb, respectively.…”

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confidence: 98%

“…Source/description: The potassium inwardly rectifying channel, subfamily J, member 8 gene (KCNJ8), previously termed KIR6.1, maps to HSA 12p11.23 and encodes an integral membrane protein and inward-rectifier type potassium channel. 1 The human gene consists of three exons spanning about 9.7 kb and the encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. 1 KCNJ8 is expressed preferentially in human heart muscle.…”

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confidence: 99%

Addition of 14 anchored loci to the porcine chromosome 8 comparative map

et al. 2004

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“…It has been shown (Jiang et al, 2002) that several genes found in the region from 16.2 to 32.2 Mb on human chromosome 1 are located in region of conserved synteny on bovine chromosome 2. The RH 5,000 map (Band et al, 2000) located six genes from this HSA1 region on BTA2.…”

Section: Discussionmentioning

confidence: 99%

A high-resolution comparative RH map of the telomeric end of bovine chromosome 2 with human chromosomes 1 and 2

Liu

Womack

Antoniou

2003

Cytogenet Genome Res

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High density livestock to human comparative maps are necessary for the implementation of comparative positional candidate gene cloning. We have constructed a high-density comparative radiation hybrid (RH) map of the telomeric end of bovine chromosome 2 (BTA2) using a 12,000-rad whole genome cattle-hamster radiation hybrid (WGRH) panel. Eighteen bovine EST markers with orthologues on human chromosomes 1 and 2 (HSA1 and HSA2), together with nine microsatellite markers, were typed against the 180 cell lines of the WGRH panel. Twenty-one markers were included in the multi-point framework map at LOD =3.0. The comparative analysis reveals a new segment of highly conserved synteny between HSA2 and BTA2.

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Measuring conservation of contiguous sets of autosomal markers on bovine and porcine genomes in relation to the map of the human genome

Cited by 10 publications

References 38 publications

Bovine and human cathelicidin cationic host defense peptides similarly suppress transcriptional responses to bacterial lipopolysaccharide

Bovine and human cathelicidin cationic host defense peptides similarly suppress transcriptional responses to bacterial lipopolysaccharide

Addition of 14 anchored loci to the porcine chromosome 8 comparative map

A high-resolution comparative RH map of the telomeric end of bovine chromosome 2 with human chromosomes 1 and 2

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