Measuring depth of injury (DOI) in an isolated rabbit eye irritation test (IRE) using biomarkers of cell death and viability

Jester, James V.; Ling, Joseph; Harbell, John W.

doi:10.1016/j.tiv.2009.10.010

Cited by 20 publications

(16 citation statements)

References 26 publications

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“…This perception is based on studies by Jester and Maurer (Jester et al, 1998a(Jester et al, ,b, 2001(Jester et al, , 2006(Jester et al, , 2010Maurer et al, 2002), who showed that the surface area and depth of initial corneal injury (DoI) in the epithelium and stroma, caused by chemicals of various classes in the rabbit eye, strongly correlate with the macroscopically observable severity and duration of ocular injury.…”

Section: Introductionmentioning

confidence: 99%

Development of an in vitro ocular test system for the prediction of all three GHS categories

Bartok

Gabel

Zorn-Kruppa

et al. 2015

Toxicology in Vitro

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Section: Introductionmentioning

confidence: 99%

Development of an in vitro ocular test system for the prediction of all three GHS categories

Bartok

Gabel

Zorn-Kruppa

et al. 2015

Toxicology in Vitro

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“…On phalloidin‐stained slides, actin cytoskeleton staining and collagen compaction were evaluated. The absence of actin cytoskeleton staining indicates cellular disintegration and, therefore, keratocyte death, which was interpreted as immediate visible effect indicating penetration depth of the CXL treatment. DAPI‐stained slides were used for the evaluation of keratocyte nucleus morphology.…”

Section: Methodsmentioning

confidence: 99%

“…However, a reliable and cost‐effective method to determine CXL penetration depth and the effect of CXL on corneal stromal cell integrity on a microscopic level has not yet been established. Jester et al . described an assessment of keratocyte death in rabbit corneas treated with different irritating materials using fluorescent biomarkers of cell death and viability.…”

Section: Introductionmentioning

confidence: 99%

Penetration depth of corneal cross‐linking with riboflavin and UV‐A (CXL) in horses and rabbits

Gallhoefer

Spiess

Guscetti

et al. 2015

Veterinary Ophthalmology

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CXL penetration depth, as determined by a novel ex vivo fluorescent assay, shows clear differences between species. A distinct effect was observed following epithelium-off CXL treatment in the anterior stroma of rabbits, but no different effect was observed in horses in comparison with nontreated controls. Different protocols need to be established to effectively treat equine patients with infectious corneal disease.

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“…For the first method, the "depth of injury" (DOI) concept, based on extensive analyses performed by Jester and colleagues on isolated rabbit eyes (Jester et al, 2010), was adapted. The depth of tissue damage after chemical exposure is determined on histological sections of the treated hemicornea models and is a measure for the eye-irritating potential of the test item.…”

Section: In Vitromentioning

confidence: 99%

In vitro eye irritation testing using the open source reconstructed hemicornea – a ring trial

Mewes

Engelke

Zorn-Kruppa

et al. 2017

ALTEX

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SummaryThe aim of the present ring trial was to test whether two new methodological approaches for the in vitro classification of eye irritating chemicals can be reliably transferred from the developers' laboratories to other sites. Both test methods are based on the well-established open source reconstructed 3D hemicornea models. In the first approach, the initial depth of injury after chemical treatment in the hemicornea model is derived from the quantitative analysis of histological sections. In the second approach, tissue viability, as a measure for corneal damage after chemical treatment, is analyzed separately for epithelium and stroma of the hemicornea model. The three independent laboratories that participated in the ring trial produced their own hemicornea models according to the test producer's instructions, thus supporting the open source concept. A total of 9 chemicals with different physicochemical and eye-irritating properties were tested to assess the between-laboratory reproducibility (BLR), the predictive performance, as well as possible limitations of the test systems. The BLR was 62.5% for the first and 100% for the second method. Both methods enabled to discriminate Cat. 1 chemicals from all non-Cat. 1 substances, which qualifies them to be used in a top-down approach. However, the selectivity between No Cat. and Cat. 2 chemicals still needs optimization.

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Measuring depth of injury (DOI) in an isolated rabbit eye irritation test (IRE) using biomarkers of cell death and viability

Cited by 20 publications

References 26 publications

Development of an in vitro ocular test system for the prediction of all three GHS categories

Development of an in vitro ocular test system for the prediction of all three GHS categories

Penetration depth of corneal cross‐linking with riboflavin and UV‐A (CXL) in horses and rabbits

In vitro eye irritation testing using the open source reconstructed hemicornea – a ring trial

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