Measuring enamel erosion: A comparative study of contact profilometry, non-contact profilometry and confocal laser scanning microscopy

Paepegaey, Aude Marine; Barker, Matthew L; Bartlett, David; Mistry, Miten; West, Nicola; Hellin, Nicola; Brown, Louise J.; Bellamy, Philip G.

doi:10.1016/j.dental.2013.09.015

Cited by 58 publications

(56 citation statements)

References 14 publications

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“…By coupling the technique of Energy Dispersive Spectroscopy (EDS) to the scanning electron microscopy (SEM) analyses of the specimens were made to determine the chemical elements present in the dentinal tubules after application of desensitizing dentifrices, taking their formulations into consideration (18). Representative samples of each group were randomly selected, in which the dentin surface profiles were obtained with a contact profilometer (Talysurf i60, Taylor Hobson, United Kingdom), in order to check the potential of the abrasive dentifrice, by assessing the loss of surface dentin structure (17,19). Qualitative analysis of the photomicrographs and the topographic images of the dentin surface were performed.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of ex vivo effectiveness of commercial desensitizing dentifrices

Mockdeci¹,

Polonini²,

Martins³

et al. 2017

J Clin Exp Dent

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BackgroundDentin hypersensitivity is a short, severe pain with fast onset. Therapy aims to either prevent or decrease neural transmission or physically occlude the dentinal tubules. This study aimed to evaluate the effectiveness of commercial desensitizing dentifrice by means of an ex vivo method.Material and MethodsSamples (n=8 lower human premolars for each group) were randomly allocated into: G1- brushing with Colgate®Sensitive Pro-Relief; G2- brushing with Sensodyne®Rapid Relief; G3- brushing with Sensodyne®Repair & Protect; and G4- brushing with Colgate®Maximum Cavity Protection. The test bodies were submitted to simulated toothbrushing and dentifrices were analyzed regarding their hydrodynamic size, polydispersity index (PI) and zeta potential. Specimens were evaluated using: scanning electron microscopy (SEM); spectroscopy energy dispersive X-ray (EDS); and profilometry. A qualitative analysis of the photomicrographs and topographies was performed.ResultsThe dentifrices showed statistical similar physical and chemical characteristics. They also demonstrated obliteration of dentinal tubules when micrographs were observed. Regarding the chemical elements present in the dentin samples, there was a statistically significant difference between the control and experimental surfaces in the four groups.ConclusionsJoint data analysis shows that the desensitizing dentifrice showed better results with regards to the obliteration of dentinal tubules compared to positive and negative controls. Key words:Dentin hypersensitivity, dentin desensitizing agents, toothpastes.

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Section: Methodsmentioning

confidence: 99%

Evaluation of ex vivo effectiveness of commercial desensitizing dentifrices

Mockdeci¹,

Polonini²,

Martins³

et al. 2017

J Clin Exp Dent

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“…The area to be treated was left exposed by placing PVC tape over the enamel surface either side of the demarcated area leaving an enamel window for treatment. The profilometer was calibrated daily on a reference block and has been validated to an accuracy of 0.042µm for the measurement of step height enamel loss [15].…”

Section: Enamel Sample Measurementmentioning

confidence: 99%

A randomised clinical trial to determine the abrasive effect of the tongue on human enamel loss with and without a prior erosive challenge

Seong¹,

Claydon²,

Macdonald³

et al. 2017

Journal of Dentistry

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. A randomised clinical trial to determine the abrasive effect of the tongue on human enamel loss with and without a prior erosive challenge. Journal of Dentistry, 58, 48-53. DOI: 10.1016/j.jdent.2017 University of Bristol -Explore Bristol Research General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms 1 A Randomised clinical trial to determine the abrasive effect of the tongue on human enamel loss with and without a prior erosive challenge ABSTRACT Objectives: To investigate the abrasive effect of the tongue on human enamel loss with and without a prior dietary acid challenge in an in situ model. Methods:A single centre, single blind, randomly allocated, split mouth, four treatment regimen, in situ study in healthy adult volunteers was undertaken. Twenty four subjects wore two lower intra-oral appliances each fitted with 4 human enamel samples 6 hours/day for 15 days. The samples were treated with either 50ml orange juice or water for 5 minutes ex vivo 4x /day; prior to being licked or not licked with the subject's tongue for 60 seconds. There were 2 samples per group per subject. Surface loss was measured by contact profilometry. Results:23 subjects completed the study with no adverse events. The mean loss of enamel at 15 days was: 0.08 μm for water without licking, 0.10 μm with water and licking; 1.55 μm with orange juice alone, 3.65 μm with orange juice and licking. In the absence of erosive challenge, licking had no detectable effect on enamel loss p=0.28. Without licking, orange juice had a highly significant effect on loss compared to water, p<0.001. Erosive challenge followed by licking more than doubled the loss of enamel p<0.001. Conclusions:When enamel was exposed to orange juice prior to licking, tissue loss as a result of tongue abrasion of the eroded surface was increased, and double that of the erosive challenge alone. Licking enamel with the tongue had no perceptible effect on enamel loss in the absence of the erosive challenge. Clinical Significance:Enamel wear resulting from tongue abrasion on tooth surfaces softened by acid challenge, can be an unavoidable consequence of oral function. This may account for the pattern of erosive toothwear on palatal and occlusal tooth surfaces, reinforcing the importance of restricting the frequency of dietary acid challenge in susceptible individuals.

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“…Any sample deformation due to an external cause being prohibited, contact profilometry [19] is not recommended, all the more so because the sample in that case needs to be fixed and held immobile. Classical optical microscopy is a simple yet efficient technique (cf.…”

Section: Classical Methods (Stoney's Formula)mentioning

confidence: 99%

“…Classical optical microscopy is a simple yet efficient technique (cf. Sections 3-4.1) but, disregarding sophisticated dynamic measurements [20,21], other methods are possible such as confocal laser scanning microscopy [19] and various forms of interferometry [22] (digital speckle interferometry [23], moiré or Fourier transformation interferometry [20]). Microcantilevers also were dealt with using vertical scanning interferometry [24].…”

Section: Classical Methods (Stoney's Formula)mentioning

confidence: 99%