2019
DOI: 10.1101/682302
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Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor

Abstract: Metabolic heterogeneity between individual cells of a population harbors offers significantchallenges for fundamental and applied research. Identifying metabolic heterogeneity and investigating its emergence requires tools to zoom into metabolism of individual cells.While methods exist to measure metabolite levels in single cells, we lack capability to measure metabolic flux, i.e. the ultimate functional output of metabolic activity, on the single-cell level. Here, combining promoter engineering, computational… Show more

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“…The number of available biosensors and reporter strains are rapidly increasing. Recently, a biosensor for the measure of glycolytic flux in yeast was developed (Monteiro et al, 2019). Combined with flow cytometry and/or microfluidics, the increase in reporter strains and biomarkers will enable the study of new phenomena and mechanisms on the single-cell level.…”
Section: Discussion Of Single-cell Technologies For the Study Of Adapmentioning
confidence: 99%
“…The number of available biosensors and reporter strains are rapidly increasing. Recently, a biosensor for the measure of glycolytic flux in yeast was developed (Monteiro et al, 2019). Combined with flow cytometry and/or microfluidics, the increase in reporter strains and biomarkers will enable the study of new phenomena and mechanisms on the single-cell level.…”
Section: Discussion Of Single-cell Technologies For the Study Of Adapmentioning
confidence: 99%