“…However, this strategy is easily adaptable to other proteins (e.g. Figure 3 D) as well, and similar strategies have been used by us and other investigators, in a range of organisms and for different applications (Jansen et al, 2007;Erhardt et al, 2008;McMurray and Thorner, 2008;Maduzia et al, 2010;Bojkowska et al, 2011;Campos et al, 2011;Dunleavy et al, 2011;Silva et al, in press; also reviewed in O' Hare et al, 2007). We will describe two typical types of SNAP-labeling strategies: Pulse-Chase (Basic Protocol 1) and Quench-Chase-Pulse (Basic Protocol 2), which allow for the analysis of old and new protein pools, respectively.…”