Measuring masses of large biomolecules and bioparticles using mass spectrometric techniques

Peng, Wen-Ping; Chou, Szu-Wei; Patil, Avinash A

doi:10.1039/c3an02329j

Cited by 26 publications

(19 citation statements)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There have been several recent reviews of high‐mass MS, but they have focused primarily on conventional MS, with only some attention paid to single‐molecule MS (Peng, Chou, & Patil, ; Snijder & Heck, ). Chang reviewed single‐molecule MS in 2009 (Chang, ), but much progress has been made since then.…”

Section: Introductionmentioning

confidence: 99%

Single‐molecule mass spectrometry

Keifer

Jarrold

2016

Mass Spectrometry Reviews

View full text Add to dashboard Cite

In single-molecule mass spectrometry, the mass of each ion is measured individually; making it suitable for the analysis of very large, heterogeneous objects that cannot be analyzed by conventional means. A range of single-molecule mass spectrometry techniques has been developed, including time-of-flight with cryogenic detectors, a quadrupole ion trap with optical detection, single-molecule Fourier transform ion cyclotron resonance, charge detection mass spectrometry, quadrupole ion traps coupled to charge detector plates, and nanomechanical oscillators. In addition to providing information on mass and heterogeneity, these techniques have been used to study impact craters from cosmic dust, monitor the assembly of viruses, elucidate the fluorescence dynamics of quantum dots, and much more. This review focuses on the merits of each of these technologies, their limitations, and their applications. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 36:715-733, 2017.

show abstract

Section: Introductionmentioning

confidence: 99%

Single‐molecule mass spectrometry

Keifer

Jarrold

2016

Mass Spectrometry Reviews

View full text Add to dashboard Cite

show abstract

“…As with the adoption of different ionization sources, the use of different mass analyzers and data acquisition systems can provide deeper structural insights into lipids of biological interest, resulting in an information-rich dataset [22]. For example, in the case of ion mobility spectrometry (IMS), an additional dimension of information is available by using chemical characterization [22,23]. …”

Section: Introductionmentioning

confidence: 99%

Lipid characterization of individual porcine oocytes by dual mode DESI-MS and data fusion

Pirro

Oliveri

Ferreira

et al. 2014

Analytica Chimica Acta

View full text Add to dashboard Cite

The development of sensitive measurements to analyze individual cells is of relevance to elucidate specialized roles or metabolic functions of each cell under physiological and pathological conditions. Lipids play multiple and critical roles in cellular functions and the application of analytical methods in the lipidomics area is of increasing interest. In this work, in vitro maturation of porcine oocytes was studied. Two independent sources of chemical information (represented by mass spectra in the positive and negative ion modes) from single oocytes (immature oocytes, 24-h and 44-h in vitro matured oocytes) were acquired by using desorption electrospray ionization-mass spectrometry (DESI-MS). Low and mid-level data fusion strategies are presented with the aim of better exploring the large amount of chemical information contained in the two mass spectrometric lipid profiles. Data were explored by principal component analysis (PCA) within the two multi-block approaches to include information on free fatty acids, phospholipids, cholesterol-related molecules, di- and triacylglycerols. After data fusion, clearer differences among immature and in vitro matured porcine oocytes were observed, which provide novel information regarding lipid metabolism throughout oocyte maturation. In particular, changes in TAG composition, as well as increase in fatty acid metabolism and membrane complexity were evidenced during the in vitro maturation process. This information can assist the improvement of in vitro embryo production for porcine species.

show abstract

“…49 Such polystyrene microspheres cover the mass range of polymeric nanomaterials investigated in our work, have low size dispersion and have been found to be softly and efficiently transferred in the gas phase by ESI. 74,75 The average mass values obtained from mass distribution for the polystyrene microsphere ions measured by CDMS using the internal calibration were plot- In CDMS, the mass m is calculated using:…”

Section: Electrospray Ionization Single-pass Cdmsmentioning

confidence: 99%

“…As a proof of concept, we demonstrated that single trapped ions can be irradiated by the CO 2 laser leading to photofragmentation patterns. Unfortunately, the "Benner trap" is more a storage device than really a MS/MS instrument, and only lim- In parallel, other technologies 75,[88][89][90][91][92][93] have recently been proposed as MS-based tools for nanometrology. For example, nanomechanical resonators with the size of several hundreds of nm have detected and measured the mass of nanoparticles.…”

Section: The Added Value Of Charge Measurementsmentioning

confidence: 99%

Weighing synthetic polymers of ultra‐high molar mass and polymeric nanomaterials: What can we learn from charge detection mass spectrometry?

Antoine

2020

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Advances in soft ionization techniques for mass spectrometry (MS) of polymeric materials make it possible to determine the masses of intact molecular ions exceeding megadaltons. Interfacing MS with separation and fragmentation methods has additionally led to impressive advances in the ability to structurally characterize polymers. Even if the gap to the megadalton range has been bridged by MS for polymers standards, the MS‐based analysis for more complex polymeric materials is still challenging. Charge detection mass spectrometry (CDMS) is a single‐molecule method where the mass and the charge of each ion are directly determined from individual measurements. The entire molecular mass distribution of a polymer sample can be thus accurately measured. Described in this perspective paper is how molecular weight distribution as well as charge distribution can provide new insights into the structural and compositional studies of synthetic polymers and polymeric nanomaterials in the megadalton to gigadalton range of molecular weight. The recent multidimensional CDMS studies involving couplings with separation and dissociation techniques will be presented. And, finally, an outlook for the future avenues of the CDMS technique in the field of synthetic polymers of ultra‐high molar mass and polymeric nanomaterials will be provided.

show abstract

Measuring masses of large biomolecules and bioparticles using mass spectrometric techniques

Cited by 26 publications

References 135 publications

Single‐molecule mass spectrometry

Single‐molecule mass spectrometry

Lipid characterization of individual porcine oocytes by dual mode DESI-MS and data fusion

Weighing synthetic polymers of ultra‐high molar mass and polymeric nanomaterials: What can we learn from charge detection mass spectrometry?

Contact Info

Product

Resources

About