Measuring Masses of Single Bacterial Whole Cells with a Quadrupole Ion Trap

Peng, Wen-Ping; Yang, Yi-Chang; Kang, Ming-Wei; Lee, Yuan T.; Chang, Huan‐Cheng

doi:10.1021/ja046754l

Cited by 58 publications

(59 citation statements)

References 28 publications

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“…We may also ask whether it is possible to launch particles from a surface in vacuum without a direct exposure to the laser. This question has been explored by several groups in physical chemistry [94] and Laser-Induced Acoustic Desorption (LIAD) has been successfully used to even load lowly charged individual viruses and bacterial cells into an ion trap [95]. Our goal here is to prepare a beam of macro- Figure 11.…”

Section: Laser-induced Acoustic Desorptionmentioning

confidence: 99%

Matter-wave physics with nanoparticles and biomolecules

Brand¹,

Eibenberger²,

Sezer³

et al. 2019

Current Trends in Atomic Physics

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These lecture notes emerged from a contribution to the "Les Houches Summer School, Session CVII-Current Trends in Atomic Physics, July 2016". It is meant to serve students as a guide to a selection of topics that are currently at the focus of molecular quantum optics and complements an earlier lecture on related topics [1]. In this review we discuss recent advances in molecular quantum optics with large organic molecules. In particular, we present successful experiments of molecules of biological importance, such as neurotransmitters and map out the route towards interferometry of large bio-matter such as proteins. The interaction of internally complex molecules with various beam splitters is discussed for both far-field diffraction at a single nanomechanical grating and the Kapitza-Dirac Talbot-Lau near-field interferometer-addressing recent progress, experimental challenges, and prospects for quantum enhanced metrology of highly complex systems in the gas phase. A central part of this review deals with the preparation of slow beams of neutral biomolecules, ranging from a single amino acid to proteins or peptides in a micro-environment. CONTENTS

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Section: Laser-induced Acoustic Desorptionmentioning

confidence: 99%

Matter-wave physics with nanoparticles and biomolecules

Brand¹,

Eibenberger²,

Sezer³

et al. 2019

Current Trends in Atomic Physics

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show abstract

“…MS has been utilized for mass measurement of single intact particles such as viruses and small cells 85, 86. Separation of microorganisms by electrophoresis has been accomplished87 and field‐flow fractionation has been combined with MALDI‐MS for analysis of intact bacteria 88.…”

Section: Sampling and Instrumentationmentioning

confidence: 99%

Mass spectrometry in biodefense

Demirev

Fenselau

2008

J. Mass Spectrom.

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Potential agents for biological attacks include both microorganisms and toxins. In mass spectrometry (MS), rapid identification of potential bioagents is achieved by detecting the masses of unique biomarkers, correlated to each agent. Currently, proteins are the most reliable biomarkers for detection and characterization of both microorganisms and toxins, and MS-based proteomics is particularly well suited for biodefense applications. Confident identification of an organism can be achieved by top-down proteomics following identification of individual protein biomarkers from their tandem mass spectra. In bottom-up proteomics, rapid digestion of intact protein biomarkers is again followed by MS/MS to provide unambiguous bioagent identification and characterization. Bioinformatics obviates the need for culturing and rigorous control of experimental variables to create and use MS fingerprint libraries for various classes of bioweapons. For specific applications, MS methods, instruments and algorithms have also been developed for identification based on biomarkers other than proteins and peptides.

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“…Microparticle quadrupole ion traps (MQITs) are also commonly used to measure the detailed properties of individual charged particles in the 100 nm to 100 µm size range, including aerosols [6,7], liquid droplets [8,9], solid particles [10,11,12], nanoparticles [13,14], and even microorganisms [15,16]. MQITs have also become popular in physics teaching, as they provide a fascinating and somewhat counterintuitive demonstration of oscillatory mechanics and electric forces.…”

Section: Introductionmentioning

confidence: 99%

Particle dynamics in damped nonlinear quadrupole ion traps

Vinitsky¹,

Black²,

Libbrecht³

2015

American Journal of Physics

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Articles you may be interested in Erratum: "Averaging peak-to-peak voltage detector for absolute mass determination of single particles with quadrupole ion traps" [Rev. Sci. Instrum.76, 023108 (2005) We examine the motions of particles in quadrupole ion traps as a function of damping and trapping forces, including cases where nonlinear damping or nonlinearities in the electric field geometry play significant roles. In the absence of nonlinearities, particles are either damped to the trap center or ejected, while their addition brings about a rich spectrum of stable closed particle trajectories. In three-dimensional (3D) quadrupole traps, the extended orbits are typically confined to the trap axis, and for this case we present a 1D analysis of the relevant equation of motion. We follow this with an analysis of 2D quadrupole traps that frequently show diamond-shaped closed orbits. For both the 1D and 2D cases, we present experimental observations of the calculated trajectories in microparticle ion traps. We also report the discovery of a new collective behavior in damped 2D microparticle ion traps, where particles spontaneously assemble into a remarkable knot of overlapping, corotating diamond orbits, self-stabilized by air currents arising from the particle motion.

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Measuring Masses of Single Bacterial Whole Cells with a Quadrupole Ion Trap

Cited by 58 publications

References 28 publications

Matter-wave physics with nanoparticles and biomolecules

Matter-wave physics with nanoparticles and biomolecules

Mass spectrometry in biodefense

Particle dynamics in damped nonlinear quadrupole ion traps

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