Measuring radial Young’s modulus of DNA by tapping mode AFM

Lin, Yi; Shen, Xin‐Cheng; Wang, Jingjing; Zhang, ZhiLing; Pang, Dai‐Wen

doi:10.1007/s11434-007-0475-7

Cited by 20 publications

(13 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5c, the Young modulus of the crystals was found to be 17.8 ± 2.5 GPa in the x-z plane, significantly higher than the values previously reported for DNA self-assembled nanostructures (Fig. 5d) [9][10][11][12][13][14][15][16][17][18] . The statistical point stiffness of the tetramer was 69.6 ± 6.8 N m −1 (Fig.…”

Section: Resultsmentioning

confidence: 59%

“…Further insights were obtained by comparing the experimental and computational Young's moduli in the x-z plane (Table 2), showing very good agreement between theory and experiment and directly confirming the anisotropic nature of Young's modulus in the plane. Specifically, experimental values in the x-z plane range between a minimum of 11.0 GPa and a [9][10][11] ; Nanowire from refs. 12 ; Nanotube from refs.…”

Section: Resultsmentioning

confidence: 99%

“…The variety and complexity, along with chemical robustness 5 , make DNA-based building blocks interesting components for nanotechnology [6][7][8] . However, the application of naturally derived DNA-based structures in material science is limited by their low stability and mechanical rigidity, with Young's moduli of 0.3-2 GPa [9][10][11][12][13][14][15][16][17][18] . Such Young's modulus values are significantly lower compared to amino acids and polyamide-based materials, such as peptides, proteins, poly-paraphenylene terephthalamide (Kev-lar®), poly(hexamethylene adipamide; Nylon 66®), and amyloidbased materials, where Young's modulus can be as much as two orders of magnitude higher [19][20][21][22][23][24][25][26][27][28] .…”

mentioning

confidence: 99%

See 2 more Smart Citations

Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid

et al. 2019

View full text Add to dashboard Cite

The variety and complexity of DNA-based structures make them attractive candidates for nanotechnology, yet insufficient stability and mechanical rigidity, compared to polyamidebased molecules, limit their application. Here, we combine the advantages of polyamide materials and the structural patterns inspired by nucleic-acids to generate a mechanically rigid fluorenylmethyloxycarbonyl (Fmoc)-guanine peptide nucleic acid (PNA) conjugate with diverse morphology and photoluminescent properties. The assembly possesses a unique atomic structure, with each guanine head of one molecule hydrogen bonded to the Fmoc carbonyl tail of another molecule, generating a non-planar cyclic quartet arrangement. This structure exhibits an average stiffness of 69.6 ± 6.8 N m −1 and Young's modulus of 17.8 ± 2.5 GPa, higher than any previously reported nucleic acid derived structure. This data suggests that the unique cation-free "basket" formed by the Fmoc-G-PNA conjugate can serve as an attractive component for the design of new materials based on PNA self-assembly for nanotechnology applications.

show abstract

Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The VSPFM method is effective in air, but not in a liquid environment. Pang et al [25] calculated the compressive Young’s modulus of ssDNA in the radial direction with the Hertz model by using the theoretical height of DNA (2 nm) and assuming that all cantilever bending energy was transferred to DNA deformation. However, some physical phenomena, such as thermal distortion and plastic deformation, consume some energy, inducing errors.…”

Section: Introductionmentioning

confidence: 99%

Measurement of Radial Elasticity and Original Height of DNA Duplex Using Tapping-Mode Atomic Force Microscopy

Zhang

Wang

et al. 2019

Nanomaterials

View full text Add to dashboard Cite

Atomic force microscopy (AFM) can characterize nanomaterial elasticity. However, some one-dimensional nanomaterials, such as DNA, are too small to locate with an AFM tip because of thermal drift and the nonlinearity of piezoelectric actuators. In this study, we propose a novel approach to address the shortcomings of AFM and obtain the radial Young’s modulus of a DNA duplex. The elastic properties are evaluated by combining physical calculations and measured experimental results. The initial elasticity of the DNA is first assumed; based on tapping-mode scanning images and tip–sample interaction force simulations, the calculated elastic modulus is extracted. By minimizing the error between the assumed and experimental values, the extracted elasticity is assigned as the actual modulus for the material. Furthermore, tapping-mode image scanning avoids the necessity of locating the probe exactly on the target sample. In addition to elasticity measurements, the deformation caused by the tapping force from the AFM tip is compensated and the original height of the DNA is calculated. The results show that the radial compressive Young’s modulus of DNA is 125–150 MPa under a tapping force of 0.5–1.3 nN; its original height is 1.9 nm. This approach can be applied to the measurement of other nanomaterials.

show abstract

“…The shortcoming of this method is that it doesn't work in liquid environment, the materials existed in liquid (such as cell, DONs) can not be measured. Pang et al [8] supposed that the bending energy of vibrated cantilever is transferred into DNA perfectly and leads to a compression, based on which they obtained the Young's modulus of DNA to be 100-300 MPa. In fact, the assumption may be not true, because the bending energy of cantilever is converted into different energy, such as thermal energy, energy for plastic deformation and so on.…”

Section: Introductionmentioning

confidence: 99%

Elasticity measurement of DNA origami nanotube in liquid with tapping mode AFM

Liu

Tabata

et al. 2014

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

View full text Add to dashboard Cite

The elasticity of nanomaterial is extremely difficult to be obtained with traditional method due to their ultra-small scale. AFM provide a feasible way to solve this problem, but it still meets many challenges especially for the measurement of one dimensional material such as DNA origami nanotube, CNT, Silicon Nanowire and so on. In addition to the influence aroused from the sample surface effect, locating the probe exactly over the 1D material with nano-diameter is hard to achieve due to the nonlinearity of PZT actuator and the thermal drift. In this study, a new method is proposed to overcome these shortcomings. It is a physical calculation process combined with experimental measurement results to deduce the elasticity of one dimensional material. This method starts at an assumed elasticity of nanomaterials, and then experimental elasticity can be obtained based on scanned image in tapping mode. The elasticity with minimized error between assumed one and experimental one should be the true value of the materials. Since with the imaging scan method, the exactly locating the probe over the sample is not necessary. In addition, due to accurately controllable tapping force, the deformation of the nanomaterial can be controlled within a tiny scale, thus the influence from the sample surface effect can be get rid of effectively. The elasticity (pre-known) of polystyrene is measured to demonstrate the effectiveness of the proposed method. The elasticity of DNA origami is also first time obtained with the proposed method, which shows an elasticity range between 75MPa and 180 MPa. This method is simple and can be used to measure other soft nano-materials.

show abstract

Measuring radial Young’s modulus of DNA by tapping mode AFM

Cited by 20 publications

References 16 publications

Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid

Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid

Measurement of Radial Elasticity and Original Height of DNA Duplex Using Tapping-Mode Atomic Force Microscopy

Elasticity measurement of DNA origami nanotube in liquid with tapping mode AFM

Contact Info

Product

Resources

About