Measuring reaction kinetics in a lab-on-a-chip by microcoil NMR

Wensink, H.; Benito‐Lopez, Fernando; Hermes, D.C.; Verboom, Willem; Gardeniers, Han; Reinhoudt, David N.; Berg, Albert van den

doi:10.1039/b414832k

Cited by 154 publications

(144 citation statements)

References 20 publications

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“…The microfluidic NMR chips were designed using CleWin Layout Editor version 4.3.3.0 (WieWeb Software, Hengelo, the Netherlands) and fabricated by Micronit Microfluidics BV, Enschede, the Netherlands, using standard microfabrication techniques similar to the ones previously described 40 . The chips have a standard format of 1.5 Â 4.5 cm, with rounded V-shaped channels wet-etched 500 mm deep in the bottom of a 1.1 mm thick borosilicate glass wafer.…”

Section: Methodsmentioning

confidence: 99%

Multinuclear nanoliter one-dimensional and two-dimensional NMR spectroscopy with a single non-resonant microcoil

Fratila

Gómez

Sýkora³

et al. 2014

Nat Commun

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Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique, but its low sensitivity and highly sophisticated, costly, equipment severely constrain more widespread applications. Here we show that a non-resonant planar transceiver microcoil integrated in a microfluidic chip (detection volume 25 nl) can detect different nuclides in the full broad-band range of Larmor frequencies (at 9.4 T from 61 to 400 MHz). Routine one-dimensional (1D) and two-dimensional (2D), homo-and heteronuclear experiments can be carried out using the broad-band coil set-up. Noteworthy, heteronuclear 2D experiments can be performed in a straightforward manner on virtually any combination of nuclides (from classical 1 H-13 C to more exotic combinations like 19 F-31 P) both in coupled and decoupled mode. Importantly, the concept of a non-resonant system provides magnetic fieldindependent NMR probes; moreover, the small-volume alleviates problems related to field inhomogeneity, making the broad-band coil an attractive option for, for example, portable and table-top NMR systems.

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Section: Methodsmentioning

confidence: 99%

Multinuclear nanoliter one-dimensional and two-dimensional NMR spectroscopy with a single non-resonant microcoil

Fratila

Gómez

Sýkora³

et al. 2014

Nat Commun

View full text Add to dashboard Cite

show abstract

“…Trumbull et al 16 integrated a planar microcoil on a capillary electrophoresis (CE) chip, demonstarting a linewidth of less than 1.5 Hz of a 30 nL sample of water. Wensink et al 15 measured reaction kinetics of imine formation from benzaldehyde and aniline. Popovic et al 17 recorded spectra of mammalian cells with a sample volume corresponding to as little as 1800 cells.…”

Section: Direct Detectionmentioning

confidence: 99%

Magnetic resonance detection: spectroscopy and imaging of lab-on-a-chip

Harel

2009

Lab Chip

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This mini-review is focused on the use of nuclear magnetic resonance (NMR) spectroscopy and imaging to study processes on lab-on-a-chip devices. NMR as an analytical tool is unmatched in its impact across nearly every area of science, from biochemistry and medicine to fundamental chemistry and physics. The controls available to the NMR spectroscopist or imager are vast, allowing for everything from high level structural determination of proteins in solution to detailed contrast imaging of organs in-vivo. Unfortunately, the weak nuclear magnetic moment of the nucleus requires that a very large number of spins be present for an inductively detectable signal, making the use of magnetic resonance as a detection modality for microfluidic devices especially challenging. Here we present recent efforts to combat the inherent sensitivity limitation of magnetic resonance for lab-on-a-chip applications. Principles and examples of different approaches are presented that highlight the flexibility and advantages of this type of detection modality.

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“…10 A microreactor chip that uses NMR detection has also been reported recently. 11 Ultrafast 2D methods have been applied to reaction transformations. 12 Finally, Hoye et al have shown that these NMR studies can easily be conducted in protonated solvents, 13 and dubbed this 'No-D' NMR.…”

Section: Introductionmentioning

confidence: 99%

Optimising reaction performance in the pharmaceutical industry by monitoring with NMR

Bernstein

Štefinović

Sleigh

2007

Magnetic Reson in Chemistry

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Quality assurance and process understanding are assuming increasing importance in the production of Active Pharmaceutical Ingredients (APIs). NMR has the potential to report on physical processes, quantities, structures, and speciation as chemical reactions progress. Following the progression of chemical reactions by placing the sample in an NMR tube, one can perform a large number of useful studies that provide chemical and mechanistic insight. But this simple approach can have limitations, and we have therefore constructed an apparatus comprising a laboratory reactor coupled with an NMR flow cell. The reactor duplicates the exact reaction conditions that will apply with large-scale production. This reaction mixture is sampled and pumped to a high-resolution NMR flow cell where the spectrum is recorded through the course of the reaction. We demonstrate the utility of reaction monitoring using NMR both for simple cases where tubes can be used, and describe the design of the on-flow apparatus and highlight its utility with an example.

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Measuring reaction kinetics in a lab-on-a-chip by microcoil NMR

Cited by 154 publications

References 20 publications

Multinuclear nanoliter one-dimensional and two-dimensional NMR spectroscopy with a single non-resonant microcoil

Multinuclear nanoliter one-dimensional and two-dimensional NMR spectroscopy with a single non-resonant microcoil

Magnetic resonance detection: spectroscopy and imaging of lab-on-a-chip

Optimising reaction performance in the pharmaceutical industry by monitoring with NMR

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