The exact determination of the rotation period is still a current and important essential issue of forestry. It attracts the attention of forest economists, managers and owners worldwide, not only of forest economists but also of forest managers. The rotation period is defined by physical, technical or financial parameters of forest management. Therefore, it is necessary to distinguish between the biological and the economic optimal rotation period. A fundamental challenge in forest management is the need for appropriate determination of the rotation period. The primary interest of our research was to compare the effective legislation for the determination of the rotation period in the Czech Republic and in the Slovak Republic. Scientific methods such as document analysis and questionnaire survey were applied. The results of the legislation analysis and other related documents were compared with the expert opinions of the relevant stakeholders. Those who affect the decision process related to the problem and those where are “affected” by the problem were involved in the study. Results show that respondents do not agree with the regulation of rotation period according to effective law. Moreover, they consider it as not usable as the conditions in forest ecosystems have changed recently, which is not considered in the legislation.