Mecanismo de acción de las fosfatasas ácidas de bajo peso molecular

Guija, Emilio; Soberón, Maria Cruz Martin; Haak-Mares, Hielke

doi:10.15381/anales.v68i4.1203

Cited by 1 publication

(2 citation statements)

References 18 publications

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“…As (>1,000 mg kg −1 ; Table S5 and S6). These metal(loid)s might interact with histidine, serine, and arginine residues in the phosphatase active site (Guija, Soberón, & Haak-Mares, 2013;Ullah & Dischinger, 1993) and hence inhibit its activity.…”

Section: Effects Of Metal(loid)s On Easmentioning

confidence: 99%

“…Several studies that evaluated the effects of individual metal(loid)s showed the inhibition of phosphatases at high contents of Cd (>100 mg kg −1 ), Pb (>1,000 mg kg −1 ), and As (>1,000 mg kg −1 ; Table S5 and S6). These metal(loid)s might interact with histidine, serine, and arginine residues in the phosphatase active site (Guija, Soberón, & Haak‐Mares, 2013; Ullah & Dischinger, 1993) and hence inhibit its activity.…”

Section: Effects Of Metal(loid)s On Easmentioning

confidence: 99%

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Soil quality indices for metal(loid) contamination: An enzymatic perspective

et al. 2020

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Soil degradation by metal and metalloid (metal[loid]) contamination represents a widespread environmental threat. Most studies investigating soil metal(loid) contamination have disregarded the soil quality concept. Enzyme activities (EAs) are good soil quality indicators due to their direct connection to functions related to C, N, P, and S cycles and their sensitivity to metal(loid) contamination. This review (a) provides an overview of the development of soil quality indices (SQIs) based on EAs in metal(loid) contaminated soils, and (b) evaluates the effects of individual metal(loid)s and their combinations on the activities of the most common enzymes involved in the C, N, P, and S cycles. Four intracellular mechanisms dominate the inhibition of EAs by metal(loid)s: (a) transcription inhibition, (b) protein denaturation, (c) cell division inhibition, (d) cell membrane disruption. These mechanisms can also be further exacerbated in soil by nonspecific exoenzyme inhibition. The main EAs used as indicators for metal(loid) contaminated soils are dehydrogenase (DHA), arylsulfatase (ARY), urease (UA), acid phosphatase (Pacid), alkaline phosphatase (Palk), and catalase (CAT). These enzymes are sensitive to metal(loid) contamination, with DHA and ARY being the most sensitive indicators (62 and 56% inhibition, respectively, when averaged over all metal[loid] contamination levels). Other enzymes are inhibited by 32% (UA), 23% (Palk), 18% (Pacid), and 18% (CAT). We suggest principles for the development of SQIs considering biotic soil functions via the use of EAs. This review is the first presenting SQIs based on EAs for metal(loid) contaminated soils, and represents the first quantitative analysis of metal(loid) effects on EAs.

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Section: Effects Of Metal(loid)s On Easmentioning

confidence: 99%

Section: Effects Of Metal(loid)s On Easmentioning

confidence: 99%