Mechanical Analysis of a Pneumatically Actuated Concentric Double-Shell Structure for Cell Stretching

Zhao, Feihu; Kreutzer, Joose; Pajunen, Sami; Kallio, Pasi

doi:10.3390/mi5040868

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…We utilized axial-symmetry and modeled a half of the structure in this study. As the membrane and the cylindrical shells were fabricated from PDMS, we used Young's modulus of 2 MPa and Poisson's ratio of 0.49 (Zhao et al 2014). The rigid structures underneath the substrate were made of PVC, and thus, we applied Young's Modulus of 3.3 GPa and Poisson's ratio of 0.4.…”

Section: Computational Simulationmentioning

confidence: 99%

Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes

Kreutzer

Viehrig

Pölönen

et al. 2019

Biomech Model Mechanobiol

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In this paper, we present a transparent mechanical stimulation device capable of uniaxial stimulation, which is compatible with standard bioanalytical methods used in cellular mechanobiology. We validate the functionality of the uniaxial stimulation system using human-induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CMs). The pneumatically controlled device is fabricated from polydimethylsiloxane (PDMS) and provides uniaxial strain and superior optical performance compatible with standard inverted microscopy techniques used for bioanalytics (e.g., fluorescence microscopy and calcium imaging). Therefore, it allows for a continuous investigation of the cell state during stretching experiments. The paper introduces design and fabrication of the device, characterizes the mechanical performance of the device and demonstrates the compatibility with standard bioanalytical analysis tools. Imaging modalities, such as high-resolution live cell phase contrast imaging and video recordings, fluorescent imaging and calcium imaging are possible to perform in the device. Utilizing the different imaging modalities and proposed stretching device, we demonstrate the capability of the device for extensive further studies of hiPSC-CMs. We also demonstrate that sarcomere structures of hiPSC-CMs organize and orient perpendicular to uniaxial strain axis and thus express more maturated nature of cardiomyocytes.

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Section: Computational Simulationmentioning

confidence: 99%

Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes

Kreutzer

Viehrig

Pölönen

et al. 2019

Biomech Model Mechanobiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The issue reports the design, fabrication and test of typical microfluidic devices for sample preparation such as micromixers [ 2 ], micropumps [ 3 , 4 ], and micro concentration gradient generators [ 5 ]. The matching size scale makes micromachines attractive tools for cell handling such as cell stretchers [ 6 ], and cell separators [ 7 ].…”

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confidence: 99%

Micromachines Beyond Silicon-Based Technologies: A Letter from the New Editor-in-Chief

Nguyen

2016

Micromachines

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It is my pleasure to assume the role of the Editor-in-Chief of Micromachines from March 2016. The journal was founded in 2009 by Prof. Miko Elwenspoek from the University of Twente, and published its first issue in 2010. Twenty years ago, I spent 6 months as a visiting PhD student in Prof. Elwenspoek's research group of Transducers Science and Technology, working on thermal flow sensors. Taking over the responsibility from Prof. Elwenspoek is my personal honour that represents a true generation change in the leadership of our editorial board.Under Prof. Elwenspoek's leadership, the journal has grown in both quantity and quality over the past five years. The number of submissions has almost doubled on a year-to-year basis. In 2015, even with a rejection rate of 52%, the number of published papers increased by 50.5% as compared to 2014. The journal has been indexed in all major databases and reached an impact factor of 1.269 (2014). As of March 2016, the journal has published 386 papers with 895 citations (778 citations without self-citations) and an H-index of 13. This performance is truly remarkable for our relatively young journal. The accomplishment was not possible without the leadership of the former Editor-in-Chief and the dedication of the highly efficient team in the editorial office. I would like to take this opportunity to thank our editorial office team for their wonderful support and dedication.The aim of Micromachines is serving the research community with an international, peer-reviewed and open-access forum for studies on micro-and nanoscale machines. Thus, traditionally the journal covers a range of topics related to Micro Electro Mechanical Systems (MEMS), Nano Electro Mechanical Systems (NEMS), Lab on a Chip (LOC), and other microdevices as well as their applications. Most of these systems and devices come from the legacy of silicon-based microtechnologies established over the past fifty years. To further improve the impact of the journal and to reach a broader audience, my vision for the journal is to broaden the subject areas beyond silicon-based microsystems and microdevices. Examples for this broader approach are bio-related applications, bio-inspired design of micromachines, micro and nanomachining of non-silicon materials, bottom-up construction of microand nanomachines. In fact, papers published in the past year already evidently supported this trend.An excellent example for biomedical applications of micromachines is the Special Issue "Biomedical Microdevices" that consists of 14 high-quality papers [1]. The issue reports the design, fabrication and test of typical microfluidic devices for sample preparation such as micromixers [2], micropumps [3,4], and micro concentration gradient generators [5]. The matching size scale makes micromachines attractive tools for cell handling such as cell stretchers [6], and cell separators [7].The design of micromachines can find inspiration from nature, as natural systems are optimised machines perfected by millions of years of evolution. The bionic mi...

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The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells

Virjula

Zhao

Leivo

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

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Mechanical Analysis of a Pneumatically Actuated Concentric Double-Shell Structure for Cell Stretching

Cited by 4 publications

References 33 publications

Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes

Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes

Micromachines Beyond Silicon-Based Technologies: A Letter from the New Editor-in-Chief

The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells

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