Mechanical and biological evaluations of novel electrospun PLLA composite scaffolds doped with oxide ceramics

Chan, Ki; Tsoi, James Kit‐Hon; Wu, On Ki; Yon, Madeline Jun Yu; Wong, Ricky Wing-Kit

doi:10.1016/j.jmbbm.2019.05.024

Cited by 22 publications

(18 citation statements)

References 32 publications

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“…SEM analysis was employed to monitor cells' attachment and morphology on the hybrid scaffolds [68][69][70]. The produced scaffolds supported the attachment and in vitro proliferation of the cultured osteoblasts.…”

Section: Sem Analysis-cell Morphology Evaluationmentioning

confidence: 99%

Mechanical Enhancement of Cytocompatible 3D Scaffolds, Consisting of Hydroxyapatite Nanocrystals and Natural Biomolecules, Through Physical Cross-Linking

et al. 2020

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Bioinspired scaffolds mimicking natural bone-tissue properties holds great promise in tissue engineering applications towards bone regeneration. Within this work, a way to reinforce mechanical behavior of bioinspired bone scaffolds was examined by applying a physical crosslinking method. Scaffolds consisted of hydroxyapatite nanocrystals, biomimetically synthesized in the presence of collagen and l-arginine. Scaffolds were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), microcomputed tomography, and nanoindentation. Results revealed scaffolds with bone-like nanostructure and composition, thus an inherent enhanced cytocompatibility. Evaluation of porosity proved the development of interconnected porous network with bimodal pore size distribution. Mechanical reinforcement was achieved through physical crosslinking with riboflavin irradiation, and nanoindentation tests indicated that within the experimental conditions of 45% humidity and 37 °C, photo-crosslinking led to an increase in the scaffold’s mechanical properties. Elastic modulus and hardness were augmented, and specifically elastic modulus values were doubled, approaching equivalent values of trabecular bone. Cytocompatibility of the scaffolds was assessed using MG63 human osteosarcoma cells. Cell viability was evaluated by double staining and MTT assay, while attachment and morphology were investigated by SEM. The results suggested that scaffolds provided a cell friendly environment with high levels of viability, thus supporting cell attachment, spreading and proliferation.

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Section: Sem Analysis-cell Morphology Evaluationmentioning

confidence: 99%

Mechanical Enhancement of Cytocompatible 3D Scaffolds, Consisting of Hydroxyapatite Nanocrystals and Natural Biomolecules, Through Physical Cross-Linking

et al. 2020

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“…This feature can be used to modify specific properties of nanofibrous polymers by increasing their mechanical strength, 26 their bioactivity, 27 and/or endowing them with additional features including electrical conductivity 28 . The chemical composition and the particle dimension strongly affect the matrix/particles interaction and, as a consequence, the final properties of polymer‐based composites 29,30 . For this reason, several inorganic nano‐sized particles, such as metals, carbon‐based materials, silica, and clays have been extensively studied in the field of polymer‐based nanocomposites in order to enhance their performances for specific applications such as environmental remediation, electromagnetic interference shielding, sensing, 31 supercapacitors, 32 packaging, automotive, and solar energy fields 25 …”

Section: Introductionmentioning

confidence: 99%

“…28 The chemical composition and the particle dimension strongly affect the matrix/particles interaction and, as a consequence, the final properties of polymer-based composites. 29,30 For this reason, several inorganic nano-sized particles, such as metals, carbon-based materials, silica, and clays have been extensively studied in the field of polymer-based nanocomposites in order to enhance their performances for specific applications such as environmental remediation, electromagnetic interference shielding, sensing, 31 supercapacitors, 32 packaging, automotive, and solar energy fields. 25 In tissue engineering applications, various types of inorganic nanofiller such as carbon nanotubes, 33 graphene, 26 and hydroxyapatite 27 have been used to produce polymer/inorganic biocomposite fibers, leading to high-performance nanofibrous mats.…”

Section: Introductionmentioning

confidence: 99%

Physical and biological properties of electrospun poly(d,l‐lactide)/nanoclay and poly(d,l‐lactide)/nanosilica nanofibrous scaffold for bone tissue engineering

Lopresti

Pavia

Ceraulo

et al. 2021

J Biomedical Materials Res

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Electrospun scaffolds exhibiting high physical performances with the ability to support cell attachment and proliferation are attracting more and more scientific interest for tissue engineering applications. The inclusion of inorganic nanoparticles such as nanosilica and nanoclay into electrospun biopolymeric matrices can meet these challenging requirements. The silica and clay incorporation into polymeric nanofibers has been reported to enhance and improve the mechanical properties as well as the osteogenic properties of the scaffolds. In this work, for the first time, the physical and biological properties of polylactic acid (PLA) electrospun mats filled with different concentrations of nanosilica and nanoclay were evaluated and compared. The inclusion of the particles was evaluated through morphological investigations and Fourier transform infrared spectroscopy. The morphology of nanofibers was differently affected by the amount and kind of fillers and it was correlated to the viscosity of the polymeric suspensions. The wettability of the scaffolds, evaluated through wet contact angle measurements, slightly increased for both the nanocomposites. The crystallinity of the systems was investigated by differential scanning calorimetry highlighting the nucleating action of both nanosilica and nanoclay on PLA. Scaffolds were mechanically characterized with tensile tests to evaluate the reinforcing action of the fillers. Finally, cell culture assays with pre‐osteoblastic cells were conducted on a selected composite scaffold in order to compare the cell proliferation and morphology with that of neat PLA scaffolds. Based on the results, we can convince that nanosilica and nanoclay can be both considered great potential fillers for electrospun systems engineered for bone tissue regeneration.

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“…Researchers have successfully fabricated multifunctional bioactive polymer membranes containing SiNPs. For example, Chan et al developed some inorganic nanoparticle (SiO2, TiO2, ZrO) loaded PLLA composite films via electrospinning for tissue engineering [31]. Antonio et al fabricated poly(εcaprolactone)/SiNPs composite membranes to guide bone regeneration [15].…”

Section: Introductionmentioning

confidence: 99%

Electrospun highly porous poly(L-lactic acid)-dopamine-SiO2 fibrous membrane for bone regeneration

Wang

Zhang

et al. 2020

Materials Science and Engineering: C

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Electrospinning has been widely used to fabricate polymer fibrous scaffolds for bone tissue engineering because of their highly porous structures. In order to improve the biocompatibility of polymer scaffolds, some nano particles have been introduced into electrospun fibres. For example, silica nanoparticles (SiNPs), with high surface area and good biocompatibility, have been used for bone tissue engineering for better bone cell attachment. In this work, porous poly(L-lactic acid) (PLLA) fibrous membrane with high surface area was fabricated by electrospinning and posttreatment process. The membrane can serve as substrates of SiNPs for bone tissue engineering. Dopamine (DOP) was applied to modify the surface of PLLA fibres, which improved the coating strength of SiNPs on PLLA fibres. SiNP coating significantly improved the mechanical properties Manuscript File Click here to view linked References 2 and hydrophilicity of PLLA/DOP/SiNP composite membranes. As a result of SiNPs coating, PLLA/DOP/SiNP membrane exhibited better cellular biocompatibility, more cell attachment and proliferation. These results demonstrate that porous PLLA/DOP/SiNP composite membrane with high surface area has high potential for periosteum in the field of bone regeneration applications.

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Mechanical and biological evaluations of novel electrospun PLLA composite scaffolds doped with oxide ceramics

Cited by 22 publications

References 32 publications

Mechanical Enhancement of Cytocompatible 3D Scaffolds, Consisting of Hydroxyapatite Nanocrystals and Natural Biomolecules, Through Physical Cross-Linking

Mechanical Enhancement of Cytocompatible 3D Scaffolds, Consisting of Hydroxyapatite Nanocrystals and Natural Biomolecules, Through Physical Cross-Linking

Physical and biological properties of electrospun poly(d,l‐lactide)/nanoclay and poly(d,l‐lactide)/nanosilica nanofibrous scaffold for bone tissue engineering

Electrospun highly porous poly(L-lactic acid)-dopamine-SiO2 fibrous membrane for bone regeneration

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