Mechanical and in vitro characterisation of decellularised porcine aortic tissue conjugated with gold nanoparticles as a vascular repair material

Ostdiek, Allison M.; Grant, Sheila; Grant, David A.

doi:10.1504/ijnbm.2015.073145

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…100 nm AuNPs at 4 times the stock solution were functionalized using a solution of 2-mercaptoethylamine and added to the tissue. The 100 nm AuNPs were utilized due to a previous study demonstrating the viability of the 100 nm AuNPs [39]. The patches were incubated for 24 hours at ambient temperature with gentle agitation followed by two 24 hour PBS rinses.…”

Section: Methodsmentioning

confidence: 99%

An in vivo study of a gold nanocomposite biomaterial for vascular repair

et al. 2015

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Currently vascular repairs are treated using synthetic or biologic patches, however these patches have an array of complications, including calcification, rupture, re-stenosis, and intimal hyperplasia. An active patch material composed of decellulzarized tissue conjugated to gold nanoparticles (AuNPs) was developed and the long term biocompatibility and cellular integration was investigated. Porcine abdominal aortic tissue was decellularized and conjugated with 100nm gold nanoparticles (AuNP). These patches were placed over a longitudinal arteriotomy of the thoracic aorta in six pigs. The animals were monitored for six months. Gross, histological, and immunohistochemical analyses of the patches were performed after euthanasia. Grossly there was minimal scar tissue with the patches still visible on the outer surface of the vessel. The inner lumen was smooth with a seamless transition from patch to native tissue. Histology demonstrated infiltration of host cells into the patch material. The immunohistochemical results demonstrated an endothelial cell layer forming over the patch within the vessel. Smooth muscle cells were repopulating the biomaterial in all animals. These results demonstrated that the AuNP biomaterial patch integrated well with the host tissue and did not failed over the six month implantation time.

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Section: Methodsmentioning

confidence: 99%

An in vivo study of a gold nanocomposite biomaterial for vascular repair

et al. 2015

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“…Conjugating different-sized gold nanoparticles (AuNPs) with decellularized porcine abdominal aortic tissue patches shows good biocompatibility when used with 100 nm AuNPs but not with 20 nm AuNPs because the latter disappeared after sterilization. 41 Such modified patches, evaluated in vivo in pigs, show good mechanical properties, promoting the growth of endothelial and smooth muscle cells when used in longitudinal arteriotomy of the thoracic aorta. 38,40 However, applying such modified matrices in human patients requires additional multifaceted studies.…”

Section: Improving Decellularized Matrices Via Npsmentioning

confidence: 99%

Incorporation of nanoparticles into transplantable decellularized matrices: Applications and challenges

Saleh

Ahmed

et al. 2018

Int J Artif Organs

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Decellularization of tissues can significantly improve regenerative medicine and tissue engineering by producing natural, less immunogenic, three-dimensional, acellular matrices with high biological activity for transplantation. Decellularized matrices retain specific critical components of native tissues such as stem cell niche, various growth factors, and the ability to regenerate in vivo. However, recellularization and functionalization of these matrices remain limited, highlighting the need to improve the characteristics of decellularized matrices. Incorporating nanoparticles into decellularized tissues can overcome these limitations because nanoparticles possess unique properties such as multifunctionality and can modify the surface of decellularized matrices with additional growth factors, which can be loaded onto the nanoparticles. Therefore, in this minireview, we highlight the various approaches used to improve decellularized matrices with incorporation of nanoparticles and the challenges present in these applications.

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