Mechanical and structural changes in rat tail tendon induced by alloxan diabetes and aging

Gałęski, Andrzej; Kastelic, J.; Baer, E.; Kohn, Robert

doi:10.1016/0021-9290(77)90091-4

Cited by 69 publications

(29 citation statements)

References 17 publications

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“…The changes in the biomechanical properties of Achilles tendons by the process of nonenzymatic glycation are consistent with the previous findings reported by the author and colleagues [23] and other investigators [22,[29][30][31]. During biomechanical testing, the coiled or crimped collagen fibrils in the tendon are expected to align along the axis of loading.…”

Section: Figuresupporting

confidence: 89%

Cross‐Linking in Collagen by Nonenzymatic Glycation Increases the Matrix Stiffness in Rabbit Achilles Tendon

Reddy

2003

Journal of Diabetes Research

213

148

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Nonenzymatic glycation of connective tissue matrix proteins is a major contributor to the pathology of diabetes and aging. Previously the author and colleagues have shown that nonenzymatic glycation significantly enhances the matrix stability in the Achilles tendon (Reddy et al., 2002, Arch. Biochem. Biophys., 399, 174-180). The present study was designed to gain further insight into glycation-induced collagen cross-linking and its relationship to matrix stiffness in the rabbit Achilles tendon. The glycation process was initiated by incubating the Achilles tendons (n = 6) in phosphate-buffered saline containing ribose, whereas control tendons (n = 6) were incubated in phosphate-buffered saline without ribose. Eight weeks following glycation, the biomechanical attributes as well as the degree of collagen cross-linking were determined to examine the potential associations between matrix stiffness and molecular properties of collagen. Compared to nonglycated tendons, the glycated tendons showed increased maximum load, stress, strain, Young's modulus of elasticity, and toughness indicating that glycation increases the matrix stiffness in the tendons. Glycation of tendons resulted in a considerable decrease in soluble collagen content and a significant increase in insoluble collagen and pentosidine. Analysis of potential associations between the matrix stiffness and degree of collagen cross-linking showed that both insoluble collagen and pentosidine exhibited a significant positive correlation with the maximum load, stress, and strain, Young's modulus of elasticity, and toughness (r values ranging from .61 to .94)

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Section: Figuresupporting

confidence: 89%

Cross‐Linking in Collagen by Nonenzymatic Glycation Increases the Matrix Stiffness in Rabbit Achilles Tendon

Reddy

2003

Journal of Diabetes Research

213

148

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“…These pathological changes in diabetes are apparently consistent with some of the changes seen in normal aging, if studied by breaking time of tendons [8,9], isometric tension [7,10] or biomechanical methods [6,11]. However, the term 'accelerated aging' is not generally accepted.…”

Section: Introductionsupporting

confidence: 58%

“…The effect of diabetes on many organs and tissues is described as accelerated aging by some authors [6][7][8] because several of the complications that affect people with diabetes, including senile cataracts, joint stiffness and atherosclerosis, are identical with disorders that develop in the elderly; they merely develop earlier in diabetics. These pathological changes in diabetes are apparently consistent with some of the changes seen in normal aging, if studied by breaking time of tendons [8,9], isometric tension [7,10] or biomechanical methods [6,11].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Normal and in vitro Aging by Non-Enzymatic Glycation as Verified by Differential Scanning Calorimetry

Reihsner

Pfeiler²,

Menzel³

1998

Gerontology

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The biomechanical parameters of rat tail tendons (RTTs) from 35-, 64-, 180- and 900-day-old animals, corresponding to the early maturation phase, the mature and the senescent state were determined. The increase of maximum stiffness, ultimate stress and the elastic fraction of stress was most pronounced in the maturation phase. Differential scanning calorimetry (DSC) experiments were performed showing an almost linear increase of the collagen denaturation temperature in the age range 35–139 days. After 14 days incubation in glucose, we observed a marked increase of the biomechanical parameters in the young, an increase of maximum stiffness in mature, and only slight alterations of the biomechanical behavior in senescent RTTs. Both glucose incorporation and formation of advanced glycation end products were most prominent in 35-day-old RTTs. These biochemical findings were in excellent agreement with the enhancement of the collagen denaturation temperature after the incubation phase. Results suggest that the validity of the term ‘accelerated aging’ depends on the experimental approach, i.e. biomechanical tests, thermal isometric contraction or DSC.

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“…In line with this, we recently demonstrated that both enzymatic hydroxylysyl pyridinoline (HP) and lysyl pyridinoline (LP) cross-links and pentosidine, which is a marker of AGE, were more abundant in the patellar tendon of old men compared with that of young men (Couppe et al 2009). It is believed that greater AGE cross-link density increases tendon stiffness (Reddy et al 2002;Galeski et al 1977;Andreassen et al 1988;Bai et al 1992), however, mechanical properties of both animal and human tendon has been reported both to increase (Nielsen et al 1998;Shadwick 1990;Kubo et al 2007a;Wood et al 2011), decrease (Stenroth et al 2012; Karamanidis and Arampatzis 2006;Dressler et al 2002;Onambele et al 2006;Mian et al 2007) or remain unaltered with aging (Couppe et al 2009;Haut et al 1992;Hubbard and SoutasLittle 1984;Johnson et al 1994;Carroll et al 2008). Thus, to what extent aging influence mechanical properties of tendon tissue remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Life-long endurance running is associated with reduced glycation and mechanical stress in connective tissue

Couppé

Svensson

Grosset

et al. 2014

AGE

112

103

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Life-long regular endurance exercise is known to counteract the deterioration of cardiovascular and metabolic function and overall mortality. Yet it remains unknown if life-long regular endurance exercise can influence the connective tissue accumulation of advanced glycation endproducts (AGEs) that is associated with aging and lifestyle-related diseases. We therefore examined two groups of healthy elderly men: 15 master athletes (64±4 years) who had been engaged in life-long endurance running and 12 old untrained (66±4 years) together with two groups of healthy young men; ten young athletes matched for running distance (26±4 years), and 12 young untrained (24±3 years). AGE cross-links (pentosidine) of the patellar tendon AGE (2014) were measured biochemically, and in the skin, it was assessed by a fluorometric method. In addition, we determined mechanical properties and microstructure of the patellar tendon. Life-long regular endurance runners (master athletes) had a 21 % lower AGE cross-link density compared to old untrained. Furthermore, both master athletes and young athletes displayed a thicker patellar tendon. These cross-sectional data suggest that life-long regular endurance running can partly counteract the aging process in connective tissue by reducing age-related accumulation of AGEs. This may not only benefit skin and tendon but also other long-lived protein tissues in the body. Furthermore, it appears that endurance running yields tendon tissue hypertrophy that may serve to lower the stress on the tendon and thereby reduce the risk of injury.

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Mechanical and structural changes in rat tail tendon induced by alloxan diabetes and aging

Cited by 69 publications

References 17 publications

Cross‐Linking in Collagen by Nonenzymatic Glycation Increases the Matrix Stiffness in Rabbit Achilles Tendon

Cross‐Linking in Collagen by Nonenzymatic Glycation Increases the Matrix Stiffness in Rabbit Achilles Tendon

Comparison of Normal and in vitro Aging by Non-Enzymatic Glycation as Verified by Differential Scanning Calorimetry

Life-long endurance running is associated with reduced glycation and mechanical stress in connective tissue

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