Mechanical Approaches to Elucidate Mechanisms of Chromosome Condensation at the Nano- and Microlevels

Kawabata, Kazushige; Nomura, Keisuke; Ikeda, Kensuke S.; Hoshi, Osamu; Fukushi, Daisuke; Haga, Hisashi; Ushiki, Tatsuo

doi:10.1201/9781420044928-2

Cited by 2 publications

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“…The paired chromatids are often connected with entangled chromatin fibers, suggesting the presence of the catenation even in the metaphase chromosome (Hoshi et al 2007b. ) images obtained by this method can visualize differences in surface stiffness or local elasticity of single human chromosomes (Nomura et al 2005;Kawabata et al 2007). On the other hand, AFM combined with scanning near-field optical microscopy (SNOM), or SNOM/AFM is one of the candidates for an attractive tool for the study of chromosomes, because it can collect both topographic and fluorescence images of the same portion of the samples simultaneously (Iwabuchi et al 1997;Yoshino et al 2002.…”

Section: Discussionmentioning

confidence: 99%

Atomic force microscopy for imaging human metaphase chromosomes

Ushiki

Hoshi

2008

Chromosome Res

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The present study introduces the principle of atomic force microscopy (AFM) and reviews our results of human metaphase chromosomes obtained by AFM. AFM imaging of the chromosomes revealed that the chromatid arm was not uniform in structure but had ridges and grooves along its length, which was most prominent in the late metaphase. The arrangement of these ridges and grooves was roughly symmetrical with the counterpart of the paired sister chromatids. AFM imaging of banded chromosomes also showed that the ridges and grooves were related to the G/Q-positive and G/Q-negative bands, respectively. At high magnification, the chromatid was seen to be produced by the compaction of highly twisted chromatin fiber loops, and its compaction tended to be stronger in the ridged regions of the chromosomes than in the grooved regions. Our AFM studies also showed the presence of catenation of chromatin fibers between the ridged portions of the chromatid in the late metaphase. Thus, AFM is useful for obtaining the three-dimensional surface topography not only in ambient conditions but also in physiological liquid conditions, and is expected to be an attractive tool for investigating the structure of chromosomes.

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Section: Discussionmentioning

confidence: 99%

Atomic force microscopy for imaging human metaphase chromosomes

Ushiki

Hoshi

2008

Chromosome Res

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“…The first studies in this area showed that the ultrastructure of the chromosomes when imaged by AFM was comparable to chromosomal images obtained with the transmission electron microscope (TEM) and scanning electron microscope (SEM) . In principle the AFM can not only provide information on the 3-D structure and topography of the chromosomes but it can also be used to give information on other surface properties like hardness and rigidity (Kawabata et al, 2007;Nomura et al, 2005). The AFM can even be used to measure single molecule interaction on the chromosome.…”

Section: Afm In Cytogeneticsmentioning

confidence: 98%

Atomic force microscopy on chromosomes, chromatin and DNA: A review

Kalle¹,

Strappe²

2012

Micron

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Mechanical Approaches to Elucidate Mechanisms of Chromosome Condensation at the Nano- and Microlevels

Cited by 2 publications

References 24 publications

Atomic force microscopy for imaging human metaphase chromosomes

Atomic force microscopy for imaging human metaphase chromosomes

Atomic force microscopy on chromosomes, chromatin and DNA: A review

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