Corn harvesting mode has gradually changed from ear harvesting to direct grain harvesting. In view of the problems of high moisture content in corn harvesting in China, such as the rates of broken grains (BGR) and uncleared grains (UGR) being too high, a new single longitudinal axial threshing cylinder was designed, which mainly included a cylinder spindle, a spiral feeding inlet, a T-type rasp bar, a separating straight rod, and a spiral extracting rod. Firstly, the three states of grain during the threshing process and the key influence factors of threshing and force analysis of corn ears in the threshing device were analyzed, then the structure of the threshing cylinder was designed, and its parameters were determined by theoretical analysis results. The arrangement mode of threshing elements adopted a combination of a T-type rasp bar, a separating straight rod, and a spiral extracting rod with a 6-head spiral pattern and an arrangement step of 250 mm. Secondly, the arrangement step of threshing elements was determined by discrete element method solution (DEM) simulation; the result showed that the average movement velocity was 55.04 m/s and the threshing time was 6–8 s. Finally, a multiple factors experiment of the threshing device was carried out, and the result showed that the order of the effect factors of the BGR and UGR was cylinder rotational speed > concave clearance > feed amount. When cylinder rotational speed was 309.17 r/min, concave clearance was 35.48 mm, and the feed amount was 6.13 kg/s. The verification experiment result showed that the BGR and UGR were 1.24% and 1.33%, respectively, which meet standard requirements. The research results could provide a reference for the design of a high moisture content grain threshing device and combine harvester.