Mechanical evaluation of a tissue-engineered zone of calcification in a bone–hydrogel osteochondral construct

Hollenstein, Jérôme; Terrier, Alexandre; Cory, Esther; Chen, Albert C.; Sah, Robert L.; Pioletti, Dominique P.

doi:10.1080/10255842.2013.794898

Cited by 9 publications

(15 citation statements)

References 27 publications

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“…This study only investigated clamping as an approach to improve mechanical properties. Other approaches such as collagen crosslinking, growth factors, and mineral gradients have shown success in other studies and could easily be applied to our experimental set up to improve mechanical properties [25,[56][57][58]. Future studies could benefit from the addition of enthesis specific cells in tissue engineered enthesis region.…”

Section: Discussionmentioning

confidence: 99%

“…Other efforts to engineer hard to soft interfaces as a model system involve creating media gradients using multi-media chamber bioreactors. The Tuan group recently developed a dual-chamber bioreactor for tissue specific media culture of an osteochondral junction [53], while a different group used a double diffusion system to create a target zone of calcification [25]. The diffusion systems focus on creating chemical gradients, while our stem uses boundary conditions to direct fiber formation into the porous bone.…”

Section: Discussionmentioning

confidence: 99%

“…Efforts to tissue engineer the ligamentous enthesis have used synthetic materials that are then seeded with coculture cell gradients [19][20][21], while other approaches utilize cellular matrix production to generate constructs from cell monolayers [22][23][24]. Osteochondral studies have developed bioreactor models that utilize diffusion systems to establish mineralization gradients in hydrogels [25,26]. While the meniscal enthesis has structural similarities to bone transition zones of ligament, tendon, and cartilage, there are distinct aspects to the meniscal attachment that necessitate a unique design approach for this interface.…”

Section: Introductionmentioning

confidence: 99%

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A model system for developing a tissue engineered meniscal enthesis

et al. 2017

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The meniscal enthesis is a complex structure that is essential to mechanical stability of the meniscus and the knee joint. Several studies document the development of anatomically shaped tissue engineered meniscus constructs, but none have focused on how to integrate such tissues with underlying bone. This study establishes a simplified construct to model the meniscal enthesis composed of a collagen gel seeded with meniscal fibrochondrocytes integrated with decellularized cancellous bone. Mechanical fixation at the bony ends induced tissue integration of fibers into the bony tissue, which is critical for mechanical performance and has yet to be shown in enthesis literature. Our test platform is amenable to targeted experiments investigating mineralization gradients, collagen fiber alignment, cell population phenotype, and media conditioning with experimental impact on enthesis studies for meniscus, tendon, and ligament.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A model system for developing a tissue engineered meniscal enthesis

et al. 2017

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“…Previously developed scaffolds have created compositional gradients by combining multiple biomaterial layers into a single construct or establishing mineral gradients through mineral growth within a single scaffold . Chemical gradients have been imposed on scaffolds through the use of diffusion systems to engineer zones of mineralization and to influence cell behavior . Interfacial scaffolds functionalized with specific regions of growth factors have been created as well to induce cellular gradients .…”

Section: Introductionmentioning

confidence: 99%

Cellular and Chemical Gradients to Engineer the Meniscus‐to‐Bone Insertion

Iannucci

Boys

McCorry

et al. 2018

Adv Healthcare Materials

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Tissue‐engineered menisci hold promise as an alternative to allograft procedures but require a means of robust fixation to the native bone. The insertion of the meniscus into bone is critical for meniscal function and inclusion of a soft tissue‐to‐bone interface in a tissue engineered implant can aid in the fixation process. The native insertion is characterized by gradients in composition, tissue architecture, and cellular phenotype, which are all difficult to replicate. In this study, a soft tissue‐to‐bone interface is tissue engineered with a cellular gradient of fibrochondrocytes and mesenchymal stem cells and subjected to a biochemical gradient through a custom media diffusion bioreactor. These constructs, consisting of interpenetrating collagen and boney regions, display improved mechanical performance and collagen organization compared to controls without a cellular or chemical gradient. Media gradient exposure produces morphological features in the constructs that appear similar to the native tissue. Collectively, these data show that cellular and biochemical gradients improve integration between collagen and bone in a tissue engineered soft tissue‐to‐bone construct.

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“…Whilst hydrogels widely appear to give the most desired matrix in terms of hypoxia, high fluid content, ease of diffusivity and tailorable mechanical properties, there is no clear consensus on what type of material is most desirable for osteochondral regeneration [13,25,26,27,28,29]. Herein we presented a hybrid construct containing both chondro- and osteo-conductive materials.…”

Section: Discussionmentioning

confidence: 99%

A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering

et al. 2016

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The concept of biphasic or multi-layered compound scaffolds has been explored within numerous studies in the context of cartilage and osteochondral regeneration. To date, no system has been identified that stands out in terms of superior chondrogenesis, osteogenesis or the formation of a zone of calcified cartilage (ZCC). Herein we present a 3D plotted scaffold, comprising an alginate and hydroxyapatite paste, cast within a photocrosslinkable hydrogel made of gelatin methacrylamide (GelMA), or GelMA with hyaluronic acid methacrylate (HAMA). We hypothesized that this combination of 3D plotting and hydrogel crosslinking would form a high fidelity, cell supporting structure that would allow localization of hydroxyapatite to the deepest regions of the structure whilst taking advantage of hydrogel photocrosslinking. We assessed this preliminary design in terms of chondrogenesis in culture with human articular chondrocytes, and verified whether the inclusion of hydroxyapatite in the form presented had any influence on the formation of the ZCC. Whilst the inclusion of HAMA resulted in a better chondrogenic outcome, the effect of HAP was limited. We overall demonstrated that formation of such compound structures is possible, providing a foundation for future work. The development of cohesive biphasic systems is highly relevant for current and future cartilage tissue engineering.

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Mechanical evaluation of a tissue-engineered zone of calcification in a bone–hydrogel osteochondral construct

Cited by 9 publications

References 27 publications

A model system for developing a tissue engineered meniscal enthesis

A model system for developing a tissue engineered meniscal enthesis

Cellular and Chemical Gradients to Engineer the Meniscus‐to‐Bone Insertion

A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering

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