Mechanical guidance of self-condensation patterns of differentiating progeny

Matsuzaki, Takahisa; Shimokawa, Yuko; Koike, Hiroyuki; Kimura, Masaki; Kawano, Yuma; Okuma, Nao; Kawamura, Ryuzo; Yoneyama, Yoshimasa; Furuichi, Yukio; Hakuno, Fumihiko; Takahashi, Shinichiro; Nakabayashi, Seiichiro; Okamoto, Satoshi; Nakauchi, Hiromitsu; Taniguchi, Hideki; Takebe, Takanori; Yoshikawa, Hiroshi

doi:10.1016/j.isci.2022.105109

Cited by 3 publications

(2 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, our group started a project for the generation of multicellular liver organoids where a hybrid model of hiPSC-derived hepatocyte and primary non-parenchymal liver cells (LSEC, HSC) are included in the same liver organoid, intended to be used as liver disease modeling tools (Figure 4A) [156,157]. The importance of having tissue-specific endothelial cells, together with the other non-parenchymal cells in the same ratio found in the liver, will allow us to recreate the native tissue microenvironment that is found in the native tissue, enabling the generation of disease models that could faithfully replicate the disease onset and progression [91,138,144,[149][150][151][152][153][154][155].…”

Section: The Role Of Endothelial Cells In Organoid Differentiation An...mentioning

confidence: 99%

“…(Figure 4B). liver disease modeling tools (Figure 4A) [156,157]. The importance of having tissue-spe-cific endothelial cells, together with the other non-parenchymal cells in the same ratio found in the liver, will allow us to recreate the native tissue microenvironment that is found in the native tissue, enabling the generation of disease models that could faithfully replicate the disease onset and progression [91,138,144,[149][150][151][152][153][154][155].…”

Section: The Role Of Endothelial Cells In Organoid Differentiation An...mentioning

confidence: 99%

See 1 more Smart Citation

Multicellular Liver Organoids: Generation and Importance of Diverse Specialized Cellular Components

Ietto

Iori

Gritti

et al. 2023

Cells

View full text Add to dashboard Cite

Over 40,000 patients in the United States are estimated to suffer from end-stage liver disease and acute hepatic failure, for which liver transplantation is the only available therapy. Human primary hepatocytes (HPH) have not been employed as a therapeutic tool due to the difficulty in growing and expanding them in vitro, their sensitivity to cold temperatures, and tendency to dedifferentiate following two-dimensional culture. The differentiation of human-induced pluripotent stem cells (hiPSCs) into liver organoids (LO) has emerged as a potential alternative to orthotropic liver transplantation (OLT). However, several factors limit the efficiency of liver differentiation from hiPSCs, including a low proportion of differentiated cells capable of reaching a mature phenotype, the poor reproducibility of existing differentiation protocols, and insufficient long-term viability in vitro and in vivo. This review will analyze various methodologies being developed to improve hepatic differentiation from hiPSCs into liver organoids, paying particular attention to the use of endothelial cells as supportive cells for their further maturation. Here, we demonstrate why differentiated liver organoids can be used as a research tool for drug testing and disease modeling, or employed as a bridge for liver transplantation following liver failure.

show abstract

Section: The Role Of Endothelial Cells In Organoid Differentiation An...mentioning

confidence: 99%

Section: The Role Of Endothelial Cells In Organoid Differentiation An...mentioning

confidence: 99%

Multicellular Liver Organoids: Generation and Importance of Diverse Specialized Cellular Components

Ietto

Iori

Gritti

et al. 2023

Cells

View full text Add to dashboard Cite

show abstract

Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

Matsuzaki,

Kawano,

Horikiri

et al. 2023

STAR Protocols

View full text Add to dashboard Cite

Quantitative Measurement of Cell Adhesion in Multicellular Systems: Toward Applications for Tissue Engineering and Bioelectronics

MATSUZAKI,

Y. YOSHIKAWA

2023

KAGAKU TO SEIBUTSU

View full text Add to dashboard Cite

Mechanical guidance of self-condensation patterns of differentiating progeny

Cited by 3 publications

References 37 publications

Multicellular Liver Organoids: Generation and Importance of Diverse Specialized Cellular Components

Multicellular Liver Organoids: Generation and Importance of Diverse Specialized Cellular Components

Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

Quantitative Measurement of Cell Adhesion in Multicellular Systems: Toward Applications for Tissue Engineering and Bioelectronics

Contact Info

Product

Resources

About