Rather than land lling or incineration, value exists in textile wastes that should be recovered. Cotton is a major component of apparel and other textile materials that could nd new value by extracting it from textile waste in the form of cotton ber fragments (CFFs). To explore this potential, CFF properties were analyzed and compared after producing them from model undyed and ber reactive dyed textile waste fabrics by mechanical milling or enzymatic degradation. Characterization methods included scanning electron microscopy (SEM), optical microscopy (OM), ber quality analysis (FQA), degree of polymerization (DP), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). Enzyme-treated undyed CFFs exhibited distinct properties of attened ber fragment dimensions, a more uniform ber length distribution compared to other CFFs, a DP comparable to solvent-spun regenerated cellulose bers from cotton, the highest crystallinity of all CFFs tested, greater hydrophobicity than milled cotton reference material, and better thermal stability than milled reference cotton. The effects of mono-functional and bi-functional reactive dyes on enzyme degradation behavior and nal product characteristics were also evaluated and discussed. Both dyed and undyed CFFs show good potential for being utilized in diverse applications. This analysis of the properties of partially degraded cotton bers provides a necessary technical foundation for promoting CFF recycling for use in applications such as composites and regenerated bers, and provides insights on the mechanism by which cotton disintegrates in the presence of enzymes to potentially inspire new strategies for dyeing cotton that takes a designed for recycling perspective into account.