Mechanical Properties of Bone in a Paraplegic Rat Model

Sugawara, Hidekazu; Linsenmeyer, Todd A.; Beam, Heather; Parsons, J. R.

doi:10.1080/10790268.1998.11719539

Cited by 12 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sugawara and colleagues have shown in adult Tx rats, a 50% reduction of femoral bone strength (compressive load to fracture) using a three-point bending system 24 weeks after trauma (Sugawara et al, 1998). Of interest, similar changes in a comparable model were reported as early as 2 weeks post-Tx (Minematsu et al, 2003), strongly suggesting that the results presented here in Tx mice were likely associated also with changes in femoral bone strength.…”

Section: Discussionsupporting

confidence: 67%

See 1 more Smart Citation

Histomorphometric and Densitometric Changes in the Femora of Spinal Cord Transected Mice

Picard

Lapointe

Brown

et al. 2008

The Anatomical Record

View full text Add to dashboard Cite

Spinal cord injury (SCI) leads generally to significant bone tissue loss within a few months to a few years post-trauma. Although, increasing data from rat models are available to study the underlying mechanisms of SCI-associated bone loss, little is known about the extent and rapidity of bone tissue changes in mouse models of SCI. The objectives are to characterize and describe quantitatively femoral bone tissue changes during 1 month in adult paraplegic mice. Histomorphometric and densitometric measurements were performed in 3-to 4-month-old CD1 mice spinal cord transected at the low-thoracic level (Th9/10). We found a general decrease in bone volume (222%), trabecular thickness (210%), and trabecular number (214%) within 30 days post-transection. Dual-energy Xray absorptiometric measurements revealed no change in bone mineral density but a significant reduction (214%) in bone mineral content. These results show large structural changes occurring within only a few weeks post-spinal cord transection in the femora of adult mice. Given the increasing availability of genetic and molecular research tools for research in mice, this murine model may be useful to study further the cellular and molecular mechanisms of demineralization associated with SCI.

show abstract

Section: Discussionsupporting

confidence: 67%

“…Although, increasing data are available from spinal Tx rats (Sugawara et al, 1998;Minematsu et al, 2003), little is known in spinal Tx mice. Recent experiments in mouse models of disuse have already led to meaningful insights into bone turnover processes and plasticity after immobilization (Priemel et al, 2002;Judex et al, 2004).…”

mentioning

confidence: 99%

Histomorphometric and Densitometric Changes in the Femora of Spinal Cord Transected Mice

Picard

Lapointe

Brown

et al. 2008

The Anatomical Record

View full text Add to dashboard Cite

show abstract

“…The animal literature contains several studies describing the natural course of bone loss in rats following SCI ( Jiang et al, 2006b( Jiang et al, ,2007a( Jiang et al, ,2007b( Jiang et al, ,2007cLiu et al, 2008aLiu et al, ,2008bMinematsu et al, 2003;Morse et al, 2008;Sugawara et al, 1998). These studies, however, did not investigate the relationship between injury severity and the degree or pattern of bone loss.…”

Section: Introductionmentioning

confidence: 99%

Bone Loss following Spinal Cord Injury in a Rat Model

Voor

Brown

et al. 2012

Journal of Neurotrauma

View full text Add to dashboard Cite

The current study was undertaken to follow the time course of bone loss in the proximal tibia of rats over several weeks following thoracic contusion spinal cord injury (SCI) of varying severity. It was hypothesized that bone loss would be more pronounced in the more severely injured animals, and that hindlimb weight bearing would help prevent bone loss. Twenty-six female Sprague-Dawley rats (200-225 g, 6-7 weeks old) received standard thoracic (T9) injuries at energies of 6.25, 12.5, 25, or 50 g-cm. The rats were scored weekly for hindlimb function during locomotion. At 0, 2 or 3, and 8 weeks, high-resolution micro-CT images of each right tibia were obtained. Mechanical indentation testing was done to measure the compressive strength of the cancellous bone structure. The 6.25 g-cm group showed near normal locomotion, the 12.5 and 25 g-cm groups showed the ability to frequently or occasionally generate weight-supported plantar steps, respectively, and the 50 g-cm group showed only movement without weight-supported plantar stepping. The 6.25, 12.5 and 25 g-cm groups remained at the same level of bone volume fraction (cancBV/TV=0.24±0.07), while the 50 g-cm group experienced severe bone loss (67%), resulting in significantly lower (p<0.05) bone volume fraction (cancBV/TV=0.11±0.05) at 8 weeks. Proximal tibia cancellous bone strength was reduced by approximately 50% in these severely injured rats. Instead of a linear proportionality between injury severity and bone loss, there appears to be a distinct functional threshold, marked by occasional weight-supported stepping, above which bone loss does not occur.

show abstract

“…In a study conducted by Sugawara et al . [13] significant differences between SCI and control rats were found in maximum torque needed to produce failure in the femoral shaft and in compressive load to produce failure in cross-sectional specimens of the distal femur and proximal tibia only at 24 weeks after injury. It is important to stress that site-specific changes have also been found from different strains, suggesting that genetics can influence bone morphology and define bone response to mechanical unloading [14].…”

Section: Discussionmentioning

confidence: 99%

Experimental research Temporal modifications in bone following spinal cord injury in rats

Medalha¹,

Amorim²,

Fernandes³

et al. 2012

aoms

View full text Add to dashboard Cite

IntroductionThe aim of this study was to investigate the temporal modifications in bone mass, bone biomechanical properties and bone morphology in spinal cord injured rats 2, 4 and 6 weeks after a transection.Material and methodsControl animals were randomly distributed into four groups (n = 10 each group): control group (CG) – control animals sacrificed immediately after surgery; spinal cord-injured 2 weeks (2W) – spinal cord-injured animals sacrificed 2 weeks after surgery; spinal cord-injured 4 weeks (4W) – spinal cord-injured animals sacrificed 4 weeks after surgery; spinal cord-injured 6 weeks (6W) – spinal cord-injured animals sacrificed 6 weeks after surgery.ResultsBiomechanical properties of the right tibia were determined by a three-point bending test and injured animals showed a statistically significant decrease in maximal load compared to control animals. The right femur was used for densitometric analysis and bone mineral content of the animals sacrificed 4 and 6 weeks after surgery was significantly higher compared to the control animals and animals sacrificed 2 weeks after surgery. Histopathological and morphological analysis of tibiae revealed intense resorptive areas in the group 2 weeks after injury only.ConclusionsThe results of this study show that this rat model is a valuable tool to investigate bone remodeling processes specifically associated with SCI. Taken together, our results suggest that spinal cord injury induced bone loss within 2 weeks after injury in rats.

show abstract

Mechanical Properties of Bone in a Paraplegic Rat Model

Cited by 12 publications

References 0 publications

Histomorphometric and Densitometric Changes in the Femora of Spinal Cord Transected Mice

Histomorphometric and Densitometric Changes in the Femora of Spinal Cord Transected Mice

Bone Loss following Spinal Cord Injury in a Rat Model

Experimental research Temporal modifications in bone following spinal cord injury in rats

Contact Info

Product

Resources

About