Mechanical properties of cellular nucleus characterized by Brillouin flow cytometry

Zhang, Jitao; Nou, Xuefei A.; Kim, Hanyoup; Scarcelli, Giuliano

doi:10.1364/fio.2017.jtu2a.84

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…BS has been combined with other types of imaging and spectroscopic techniques to provide a further and complete analysis of cellular samples. In 2017, spontaneous BS was coupled with flow cytometry to determine the effect of relevant physiological changes on nuclear stiffness [ 71 ]. Using VIPA spectrometers, fast sampling times, and submicron laser beams, NIH/3T3 fluorescent cells flowing through a microchannel were individually analyzed by BS and spatially co-registered by a fluorescence microscope incorporated into the setup [ 63 ].…”

Section: Biological Applications Of Brillouinmentioning

confidence: 99%

Brillouin Spectroscopy: From Biomedical Research to New Generation Pathology Diagnosis

Riobóo

Gontán

Sanderson

et al. 2021

IJMS

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Brillouin spectroscopy has recently gained considerable interest within the biomedical field as an innovative tool to study mechanical properties in biology. The Brillouin effect is based on the inelastic scattering of photons caused by their interaction with thermodynamically driven acoustic modes or phonons and it is highly dependent on the material’s elasticity. Therefore, Brillouin is a contactless, label-free optic approach to elastic and viscoelastic analysis that has enabled unprecedented analysis of ex vivo and in vivo mechanical behavior of several tissues with a micrometric resolution, paving the way to a promising future in clinical diagnosis. Here, we comprehensively review the different studies of this fast-moving field that have been performed up to date to provide a quick guide of the current literature. In addition, we offer a general view of Brillouin’s biomedical potential to encourage its further development to reach its implementation as a feasible, cost-effective pathology diagnostic tool.

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Section: Biological Applications Of Brillouinmentioning

confidence: 99%

Brillouin Spectroscopy: From Biomedical Research to New Generation Pathology Diagnosis

Riobóo

Gontán

Sanderson

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…The ability to measure changes in the stiffness across cells in a contact-less, label-free manner opens up a plethora of Brillouin analysis-based applications for study of cellular biomechanics in disease and therapeutic development. Zhang et al demonstrate the extension of Brillouin microscopy to a flow cytometry technique capable of classifying cell populations based on nuclear mechanical signatures when flowed through a microfluidic device [76]. In [7], treatment of fibroblast cells with trichostatin-A, a chromatin decondensation drug, were shown to cause a significant reduction in nuclear stiffness, allowing successful differentiation from populations of untreated cells.…”

Section: Single Cellsmentioning

confidence: 99%

Brillouin imaging for studies of micromechanics in biology and biomedicine: from current state-of-the-art to future clinical translation

et al. 2020

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Brillouin imaging is increasingly recognized to be a powerful technique that enables noninvasive measurement of the mechanical properties of cells and tissues on a microscopic scale. This provides an unprecedented tool for investigating cell mechanobiology, cell-matrix interactions, tissue biomechanics in healthy and disease conditions and other fundamental biological questions. Recent advances in optical hardware have particularly accelerated the development of the technique, with increasingly finer spectral resolution and more powerful system capabilities. We envision that further developments will enable translation of Brillouin imaging to assess clinical specimens and samples for disease screening and monitoring. The purpose of this review is to summarize the stateof-the-art in Brillouin microscopy and imaging with a specific focus on biological tissue and cell measurements. Key system and operational requirements will be discussed to facilitate wider application of Brillouin imaging along with current challenges for translation of the technology for clinical and medical applications.

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Mechanical properties of cellular nucleus characterized by Brillouin flow cytometry

Cited by 2 publications

References 6 publications

Brillouin Spectroscopy: From Biomedical Research to New Generation Pathology Diagnosis

Brillouin Spectroscopy: From Biomedical Research to New Generation Pathology Diagnosis

Brillouin imaging for studies of micromechanics in biology and biomedicine: from current state-of-the-art to future clinical translation

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