Mechanical properties of single alginate microspheres determined by microcompression and finite element modelling

Nguyen, Van Bo; Wang, C.X.; Thomas, C. R.; Zhang, Zhibing

doi:10.1016/j.ces.2008.10.050

Cited by 50 publications

(35 citation statements)

References 41 publications

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“…According to the stress-strain curves of the compressed samples, the displacement of this elastic model was defined as 25%. A Poisson ratio of 0.31 was selected for alginate from the literature (Nguyen et al, 2009;Zhang et al, 2013) and ΔL was defined as the value of deformed sections at the top and bottom of the scaffold. Finally, Ex and Ez were defined in the model so that the real structure of the printed scaffold could be represented (Figure 2).…”

Section: Design and Fabrication Of Alginate Scaffoldsmentioning

confidence: 99%

Influence of crosslinking on the mechanical behavior of 3D printed alginate scaffolds: Experimental and numerical approaches

Naghieh

Karamooz-Ravari

Sarker

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

108

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Tissue scaffolds fabricated by three-dimensional (3D) bioprinting are attracting considerable attention for tissue engineering applications. Because the mechanical properties of hydrogel scaffolds should match the damaged tissue, changing various parameters during 3D bioprinting has been studied to manipulate the mechanical behavior of the resulting scaffolds. Crosslinking scaffolds using a cation solution (such as CaCl 2 ) is also important for regulating the mechanical properties, but has not been well documented in the literature. Here, the effect of varied crosslinking agent volume and crosslinking time on the mechanical behavior of 3D bioplotted alginate scaffolds was evaluated using both experimental and numerical methods. Compression tests were used to measure the elastic modulus of each scaffold, then a finite element model was developed and a power model used to predict scaffold mechanical behavior. Results showed that crosslinking time and volume of crosslinker both play a decisive role in modulating the mechanical properties of 3D bioplotted scaffolds. Because mechanical properties of scaffolds can affect cell response, the findings of this study can be implemented to modulate the elastic modulus of scaffolds according to the intended application.

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Section: Design and Fabrication Of Alginate Scaffoldsmentioning

confidence: 99%

Influence of crosslinking on the mechanical behavior of 3D printed alginate scaffolds: Experimental and numerical approaches

Naghieh

Karamooz-Ravari

Sarker

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

108

View full text Add to dashboard Cite

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“…The recent work of Nguyen et al (2009) on FEM compression of alginate spheres was extended to simulate the compression of coreshell microcapsules using ABAQUS/Standard 6.5. The wall of the microcapsules was designed as in Rachik et al (2006) following two approaches: (1) using shell finite elements and (2) using standard solid elements.…”

Section: Fem Simulations Using Abaqusmentioning

confidence: 99%

“…FEM has briefly been used in the past for the analysis compression of cells and capsules (Rachik et al, 2006;Smith et al, 1998;Nguyen et al, 2009), yet the effect of the shell thickness has not been investigated in detail at small deformations ( o0.2).…”

Section: Introductionmentioning

confidence: 99%

Determination of the elastic properties of single microcapsules using micromanipulation and finite element modeling

Mercadé‐Prieto

Nguyen²,

Allen³

et al. 2011

Chemical Engineering Science

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“…Compression testing by micromanipulation can be used to determine the mechanical properties of small particles or cells with sizes down to about 1 lm. [14][15][16] It has been used to study many experimental systems including sea-urchin eggs, 17,18 single tomato cells, [19][20][21] animal cells, 16,22,23 yeast cells, [7][8][9][10][11][12][13] bacteria, 24 microspheres and microcapsules, 15,25,26 and pollen grains. 27 In this technique, an individual cell is compressed between two flat parallel surfaces, usually until it bursts.…”

Section: Introductionmentioning

confidence: 99%

Determining the mechanical properties of yeast cell walls

Stenson

Hartley

Wang

et al. 2011

Biotechnology Progress

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The intrinsic cell wall mechanical properties of Baker's yeast (Saccharomyces cerevisiae) cells were determined. Force-deformation data from compression of individual cells up to failure were recorded, and these data were fitted by an analytical model to extract the elastic modulus of the cell wall and the initial stretch ratio of the cell. The cell wall was assumed to be homogeneous, isotropic, and incompressible. A linear elastic constitutive equation was assumed based on Hencky strains to accommodate the large stretches of the cell wall. Because of the high compression speed, water loss during compression could be assumed to be negligible. It was then possible to treat the initial stretch ratio and elastic modulus as adjustable parameters within the analytical model. As the experimental data fitted numerical simulations well up to the point of cell rupture, it was also possible to extract cell wall failure criteria. The mean cell wall properties for resuspended dried Baker's yeast were as follows: elastic modulus 185 ± 15 MPa, initial stretch ratio 1.039 ± 0.006, circumferential stress at failure 115 ± 5 MPa, circumferential strain at failure 0.46 ± 0.03, and strain energy per unit volume at failure 30 ± 3 MPa. Data on yeast cells obtained by this method and model should be useful in the design and optimization of cell disruption equipment for yeast cell processing.

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Mechanical properties of single alginate microspheres determined by microcompression and finite element modelling

Cited by 50 publications

References 41 publications

Influence of crosslinking on the mechanical behavior of 3D printed alginate scaffolds: Experimental and numerical approaches

Influence of crosslinking on the mechanical behavior of 3D printed alginate scaffolds: Experimental and numerical approaches

Determination of the elastic properties of single microcapsules using micromanipulation and finite element modeling

Determining the mechanical properties of yeast cell walls

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