Mechanical properties of single electrospun collagen type I fibers

Yang, Lanti; Fitié, Carel F. C.; Werf, Kees O. van der; Bennink, Martin L.; Dijkstra, Pieter J.; Feijén, Jan

doi:10.1016/j.biomaterials.2007.10.058

Cited by 267 publications

(246 citation statements)

References 40 publications

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“…With respect to fabrication methods, electro-spinning (Figure 4) appears to be the method of choice, as aligned nano-to micro-fibrous scaffolds can be produced with mechanical and structural features similar to the tissue to be replaced and with ability to control permanently differentiated and stem cell fate in vitro; to locally deliver in a controlled manner pharmacological agents, biological molecules and cells at the side of injury; and to enhance directional neotissue formation [404][405][406][407][408][409]. Although electro-spun collagen scaffolds have demonstrated improved neotissue alignment and mechanical properties, over control counterparts in a rabbit Achilles tendon model [410] the realisation that the use of fluoro-alcohols denatures the triple helical confirmation of collagen [411,412] has reduced further investigations.…”

Section: Bottom-up Approached For Tendon Repair Based On Synthetic Inmentioning

confidence: 99%

The past, present and future in scaffold-based tendon treatments

Lomas

Ryan

Sorushanova

et al. 2015

Advanced Drug Delivery Reviews

183

188

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Section: Bottom-up Approached For Tendon Repair Based On Synthetic Inmentioning

confidence: 99%

The past, present and future in scaffold-based tendon treatments

Lomas

Ryan

Sorushanova

et al. 2015

Advanced Drug Delivery Reviews

183

188

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“…[40][41][42] Not only can HFIP and TFE induce an apparent loss of the triple helical configuration of collagen, but they can remain a highly volatile and corrosive solvent that poses health risks to humans, as well as its high price. [43][44][45][46] In consideration of the shortcoming of HFIP, several attempts have recently been made at finding more eco and environmentally benign solvents to prepare collagen solutions for electrospinning. For example, various acids such as formic acid 47,48 and acetic acid, 49,50 were investigated as less cytotoxic solvents for electrospinning collagen nanofibers.…”

Section: Zhou Et Almentioning

confidence: 99%

Greener synthesis of electrospun collagen/hydroxyapatite composite fibers with an excellent microstructure for bone tissue engineering

Zhou¹,

Yao²,

Wang³

et al. 2015

IJN

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In bone tissue engineering, collagen/hydroxyapatite (HAP) fibrous composite obtained via electrospinning method has been demonstrated to support the cells’ adhesion and bone regeneration. However, electrospinning of natural collagen often requires the use of cytotoxic organic solvents, and the HAP crystals were usually aggregated and randomly distributed within a fibrous matrix of collagen, limiting their clinical potential. Here, an effective and greener method for the preparation of collagen/HAP composite fibers was developed for the first time, and this green product not only had 40 times higher mechanical properties than that previously reported, but also had an excellent microstructure similar to that of natural bone. By dissolving type I collagen in environmentally friendly phosphate buffered saline/ethanol solution instead of the frequently-used cytotoxic organic solvents, followed with the key step of desalination, co-electrospinning the collagen solution with the HAP sol, generates a collagen/HAP composite with a uniform and continuous fibrous morphology. Interestingly, the nano-HAP needles were found to preferentially orient along the longitudinal direction of the collagen fibers, which mimicked the nanostructure of natural bones. Based on the characterization of the related products, the formation mechanism for this novel phenomenon was proposed. After cross-linking with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride/ N -hydroxysuccinimide, the obtained composite exhibited a significant enhancement in mechanical properties. In addition, the biocompatibility of the obtained composite fibers was evaluated by in vitro culture of the human myeloma cells (U2-OS). Taken together, the process outlined herein provides an effective, non-toxic approach for the fabrication of collagen/HAP composite nanofibers that could be good candidates for bone tissue engineering.

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“…Instead of electrospinning collagen from calf skin using 1,1,1,3,3,3-hexafluoroisopropanol as a solvent, 8 in our study, only one organic solvent, methanoic acid, with relatively lower toxicity, was used for FSCP/ COS electrospinning. The FSCP/COS f ibers have smaller dimensions than mammal collagen fibers from calf skin, with diameters ranging from 50 to 100 nm ( Figure 1E).…”

Section: Electrospun Fscp/cos Fibersmentioning

confidence: 99%

“…The FSCP/COS f ibers have smaller dimensions than mammal collagen fibers from calf skin, with diameters ranging from 50 to 100 nm ( Figure 1E). 8 To evaluate the composite NFM and its constituent polymers, FTIR spectra were used. After mixing with COS and PVA, the IR spectrum of FSCP changed markedly (Figure 2).…”

Section: Electrospun Fscp/cos Fibersmentioning

confidence: 99%

“…8,9 In recent years the occurrence of human-animal cross-infections has increased concern about using collagen extracts from terrestrial animal sources. 10 Consequently, extracts from fish skins, scales, and bones have become a potential alternative source for collagen, and may be superior to mammalian collagen because of the absence of the risk of animalrelated contamination.…”

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confidence: 99%

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Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing

Li¹,

Wang²

2011

IJN

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Purpose: The objective of the present investigation was to evaluate the antibacterial properties and the biocompatibility of composite electrospun nanofibrous membranes (NFMs) with low-molecular-weight fish scale collagen peptides (FSCP) and chito-oligosaccharide (COS), to determine their potential for use as wound dressings. Methods: Low-molecular-weight FSCP were combined with COS to prepare nanofibers by electrospinning, and polyvinyl alcohol (PVA) was used for enhancing fiber-forming ability. Transmission electron microscope and scanning electron microscope methods were used to observe bacterial adhesion and the bacterial cell membrane. Fibroblast cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The best FSCP/COS mass ratio for electrospinning was 2:1, and the nanofibers had small dimensions ranging from 50 to 100 nm. The NFM showed good antibacterial activities against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The antibacterial activity against S. aureus was higher than against E. coli . The pili and adhesive fimbriae of E. coli promoted bacterial adhesion to the NFM surfaces, and S. aureus biofilms aided S. aureus adhesion on the surface of NFMs. Damage to the bacterial cell membrane indicates that the NFMs could lead to the release of intracellular materials, particularly with S. aureus . In addition, FSCP/COS NFM rapidly increased the permeability of the outer membranes of E. coli . The electrospun NFM with FSCP and COS had good biocompatibility in vitro and supported proliferation of human skin fibroblasts. Conclusion: FSCP are superior to mammalian collagen, and have feasibility and potency for wound dressings. FSCP/COS NFMs had good anti-bactericidal activity that improved with increased COS, and showed good biocompatibility in vitro and supported the proliferation of fibroblasts.

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Mechanical properties of single electrospun collagen type I fibers

Cited by 267 publications

References 40 publications

The past, present and future in scaffold-based tendon treatments

The past, present and future in scaffold-based tendon treatments

Greener synthesis of electrospun collagen/hydroxyapatite composite fibers with an excellent microstructure for bone tissue engineering

Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing

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