Mechanical Responses of Cancer Cells on Nanoscaffolds for Adhesion Size Control

Park, Soyeun; Bastatas, Lyndon D.; Matthews, James R.; Lee, Yong Joong

doi:10.1002/mabi.201400504

Cited by 7 publications

(4 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, AFM has been used for investigating the mechanical response of cancer cells on different substrates. Park et al studied prostate and breast cancer cells that were cultured on nanoscafolds and showed that different cancer cell lines have distinct response to substrate characteristics such as size and nanomechanical properties [175]. Additionally, AFM can be used for studying cell-cell interaction.…”

Section: Afm and Specific Pathological Conditionsmentioning

confidence: 99%

Atomic Force Microscopy on Biological Materials Related to Pathological Conditions

et al. 2019

View full text Add to dashboard Cite

Atomic force microscopy (AFM) is an easy-to-use, powerful, high-resolution microscope that allows the user to image any surface and under any aqueous condition. AFM has been used in the investigation of the structural and mechanical properties of a wide range of biological matters including biomolecules, biomaterials, cells, and tissues. It provides the capacity to acquire high-resolution images of biosamples at the nanoscale and allows at readily carrying out mechanical characterization. The capacity of AFM to image and interact with surfaces, under physiologically relevant conditions, is of great importance for realistic and accurate medical and pharmaceutical applications. The aim of this paper is to review recent trends of the use of AFM on biological materials related to health and sickness. First, we present AFM components and its different imaging modes and we continue with combined imaging and coupled AFM systems. Then, we discuss the use of AFM to nanocharacterize collagen, the major fibrous protein of the human body, which has been correlated with many pathological conditions. In the next section, AFM nanolevel surface characterization as a tool to detect possible pathological conditions such as osteoarthritis and cancer is presented. Finally, we demonstrate the use of AFM for studying other pathological conditions, such as Alzheimer’s disease and human immunodeficiency virus (HIV), through the investigation of amyloid fibrils and viruses, respectively. Consequently, AFM stands out as the ideal research instrument for exploring the detection of pathological conditions even at very early stages, making it very attractive in the area of bio- and nanomedicine.

show abstract

Section: Afm and Specific Pathological Conditionsmentioning

confidence: 99%

Atomic Force Microscopy on Biological Materials Related to Pathological Conditions

et al. 2019

View full text Add to dashboard Cite

show abstract

“…cell manipulations with atomic force microscope and optical tweezer techniques). 34,35 In addition to these approaches, the substrates were developed to measure traction forces exerted by the cells. Microbeads embedded elastic substrates have been used to measure the force exerted by the cells to the substrates, which is called traction force microscopy.…”

Section: Introductionmentioning

confidence: 99%

Integrin-independent Cell Adhesion Substrates: Possibility of Applications for Mechanobiology Research

Hoshiba

Tanaka

2016

ANAL. SCI.

View full text Add to dashboard Cite

“…These techniques include optical stretcher [102][103][104][105], micropipette aspiration [106], microfluidics [107], magnetic tweezers [108][109][110], and AFM [111][112][113][114]. Among these, AFM has been the most widely utilized in bio-mechanical assays [13,52,[115][116][117][118].…”

Section: Mechanical Compliancementioning

confidence: 99%

Nano-scientific Application of Atomic Force Microscopy in Pathology: from Molecules to Tissues

Kiio

Park

2020

Int. J. Med. Sci.

Self Cite

View full text Add to dashboard Cite

The advantages of atomic force microscopy (AFM) in biological research are its high imaging resolution, sensitivity, and ability to operate in physiological conditions. Over the past decades, rigorous studies have been performed to determine the potential applications of AFM techniques in disease diagnosis and prognosis. Many pathological conditions are accompanied by alterations in the morphology, adhesion properties, mechanical compliances, and molecular composition of cells and tissues. The accurate determination of such alterations can be utilized as a diagnostic and prognostic marker. Alteration in cell morphology represents changes in cell structure and membrane proteins induced by pathologic progression of diseases. Mechanical compliances are also modulated by the active rearrangements of cytoskeleton or extracellular matrix triggered by disease pathogenesis. In addition, adhesion is a critical step in the progression of many diseases including infectious and neurodegenerative diseases. Recent advances in AFM techniques have demonstrated their ability to obtain molecular composition as well as topographic information. The quantitative characterization of molecular alteration in biological specimens in terms of disease progression provides a new avenue to understand the underlying mechanisms of disease onset and progression. In this review, we have highlighted the application of diverse AFM techniques in pathological investigations.

show abstract

Mechanical Responses of Cancer Cells on Nanoscaffolds for Adhesion Size Control

Cited by 7 publications

References 43 publications

Atomic Force Microscopy on Biological Materials Related to Pathological Conditions

Atomic Force Microscopy on Biological Materials Related to Pathological Conditions

Integrin-independent Cell Adhesion Substrates: Possibility of Applications for Mechanobiology Research

Nano-scientific Application of Atomic Force Microscopy in Pathology: from Molecules to Tissues

Contact Info

Product

Resources

About