Mechanical stability of particle-stabilized droplets under micropipette aspiration

Samudrala, Niveditha; Nam, Jin; Sarfati, Raphaël; Style, Robert W.; Dufresne, Eric R.

doi:10.1103/physreve.95.012805

Cited by 10 publications

(11 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Essentially the same mechanical instability has been observed for liquid droplets, − neutrophils ( i.e. , certain types of white blood cells), , and fibroblasts .…”

Section: Resultssupporting

confidence: 61%

“…However, for the tubulated vesicles considered here, the mechanical tension can be ignored compared to the spontaneous tension, as shown in the SI and summarized by the relation . Therefore, the elastic counter pressure as given by eq has the asymptotic form An analogous equation applies to the micropipette aspiration of liquid droplets, − for which the counter pressure arises from capillary forces, and the spontaneous tension σ of the GUV is replaced by the interfacial tension of the droplet.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Membrane Nanotubes Increase the Robustness of Giant Vesicles

et al. 2018

View full text Add to dashboard Cite

Giant unilamellar vesicles (GUVs) provide a direct connection between the nano-and the microregime. On the one hand, these vesicles represent biomimetic compartments with linear dimensions of many micrometers. On the other hand, the vesicle walls are provided by single molecular bilayers that have a thickness of a few nanometers and respond sensitively to molecular interactions with small solutes, biopolymers, and nanoparticles. These nanoscopic responses are amplified by the GUVs and can then be studied on much larger scales. Therefore, GUVs are increasingly used as a versatile research tool for basic membrane science, bioengineering, and synthetic biology. Conventional GUVs have one major drawback, however: they have only a limited capability to cope with external perturbations such as osmotic inflation, adhesion, or micropipette aspiration that tend to rupture the membranes. In contrast, cell membranes tolerate the same kinds of mechanical perturbations without rupture because the latter membranes are coupled to reservoirs of membrane area. Here, we introduce GUVs with membrane nanotubes as model systems that include such area reservoirs. To demonstrate the increased robustness of these tubulated vesicles, we use micropipette aspiration and changes in the osmotic conditions applied to phospholipid membranes doped with the glycolipid GM1. A quantitative comparison between theory and experiment reveals that the response of the GUVs is governed by the membranes' spontaneous tension, a curvature-elastic material parameter that describes the bilayer asymmetry on the nanoscale. Because of their increased robustness, GUVs with nanotubes represent improved research tools for membrane science, in general, with potential applications as storage and delivery systems and as cell-like microcompartments in bioengineering, pharmacology, and synthetic biology.

show abstract

“…Essentially the same mechanical instability has been observed for liquid droplets, − neutrophils ( i.e. , certain types of white blood cells), , and fibroblasts .…”

Section: Resultssupporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Membrane Nanotubes Increase the Robustness of Giant Vesicles

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Several studies have confirmed such a behavior, whatever the particle size, particle shape, i.e. dumbbell [59] and ellipsoidal [4] shapes, particle nature [4,59], or bubble/drop size. We stress that all the results from literature can be gathered on the same graph by plotting the reduced collapse pressure ∆ (2 ⁄ ) ⁄ (which is equivalent to Π ⁄ ) as a function of ⁄ (see figure 2-d).…”

Section: Shrinkage Of Armored Spherical Bubbles and Dropsmentioning

confidence: 97%

Rheology of particulate rafts, films, and foams

Pitois

Rouyer

2019

Current Opinion in Colloid & Interface Science

View full text Add to dashboard Cite

Liquid foam exhibits remarkable rheological behavior although it is made with simple fluids: it behaves like a solid at low shear stress but flows like a liquid above a critical shear stress. Such properties, that have been proved to be useful for many applications, are even enhanced by adding solid particles. Depending on their hydrophobicity and their size, the particles can have different geometrical configurations at the mesoscopic scale, i.e. at the air-liquid interfaces, in the films or the interstices between the bubbles. In this review, we present rheological studies performed on granular rafts and films, on spherical armored interfaces, on gas marbles and on aqueous foams laden with hydrophilic grains.

show abstract

“…Indeed, if the area reservoir provided by the membrane nanotubes is sufficiently large, the micropipette aspiration of the vesicles leads to a mechanical instability and to the uptake of the vesicle by the micropipette, as in Figure 10e, in close analogy to the corresponding mechanical instability that has been observed during the micropipette aspiration of a liquid droplet. [ 92–94 ]…”

Section: Elasticity Of Biomembranesmentioning

confidence: 99%

Remodeling of Membrane Shape and Topology by Curvature Elasticity and Membrane Tension

Lipowsky

2021

Advanced Biology

View full text Add to dashboard Cite

Cellular membranes exhibit a fascinating variety of different morphologies, which are continuously remodeled by transformations of membrane shape and topology. This remodeling is essential for important biological processes (cell division, intracellular vesicle trafficking, endocytosis) and can be elucidated in a systematic and quantitative manner using synthetic membrane systems. Here, recent insights obtained from such synthetic systems are reviewed, integrating experimental observations and molecular dynamics simulations with the theory of membrane elasticity. The study starts from the polymorphism of biomembranes as observed for giant vesicles by optical microscopy and small nanovesicles in simulations. This polymorphism reflects the unusual elasticity of fluid membranes and includes the formation of membrane necks or fluid ‘worm holes'. The proliferation of membrane necks generates stable multi‐spherical shapes, which can form tubules and tubular junctions. Membrane necks are also essential for the remodeling of membrane topology via membrane fission and fusion. Neck fission can be induced by fine‐tuning of membrane curvature, which leads to the controlled division of giant vesicles, and by adhesion‐induced membrane tension as observed for small nanovesicles. Challenges for future research include the interplay of curvature elasticity and membrane tension during membrane fusion and the localization of fission and fusion processes within intramembrane domains.

show abstract

Mechanical stability of particle-stabilized droplets under micropipette aspiration

Cited by 10 publications

References 33 publications

Membrane Nanotubes Increase the Robustness of Giant Vesicles

Membrane Nanotubes Increase the Robustness of Giant Vesicles

Rheology of particulate rafts, films, and foams

Remodeling of Membrane Shape and Topology by Curvature Elasticity and Membrane Tension

Contact Info

Product

Resources

About