Mechanical Transmission, Purification, and Some Properties of Whitefly-Borne Mung Bean Yellow Mosaic Virus in Thailand

Honda, Y.; Iwaki, Mitsuro; Saito, Yasuo; Thongmeearkom, Pornpod; Kittisak, K.; Deema, Nualchan

doi:10.1094/pd-67-801

Cited by 50 publications

(32 citation statements)

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“…Therefore, an attempt was made to develop tobacco as a model plant to study PDR in transgenic plants harbouring MYMV genes. MYMV inoculation of tobacco (Nicotiana tabacum) did not lead to the development of viral symptoms [36]. Here, we report that tobacco leaf discs agroinoculated with partial dimers of MYMV-Vig support viral replication.…”

Section: Introductionmentioning

confidence: 82%

Expression of full-length and truncated Rep genes from Mungbean yellow mosaic virus-Vigna inhibits viral replication in transgenic tobacco

et al. 2006

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Mungbean yellow mosaic virus-Vigna (MYMV-Vig) is a bipartite geminivirus that causes a severe yellow mosaic disease in blackgram. An assay was developed to study MYMV-Vig replication by agroinoculation of tobacco leaf discs with partial dimers of the virus. This assay, in a non-host model plant, was used to evaluate pathogen-derived resistance contributed by MYMV-Vig genes in transgenic plants. Viral DNA accumulation was optimum in tobacco leaf discs cultured for 10 days after infection with Agrobacterium tumefaciens strain Ach5 containing partial dimers of both DNA A and DNA B of MYMV-Vig. Transgenic tobacco plants with MYMV-Vig genes for coat protein (CP), replication-associated protein (Rep)-sense, Rep-antisense, truncated Rep (T-Rep), nuclear shuttle protein (NSP) and movement protein (MP) were generated. Leaf discs from transgenic tobacco plants, harbouring MYMV-Vig genes, were agroinoculated with partial dimers of MYMV-Vig and analyzed for viral DNA accumulation. The leaf discs from transgenic tobacco plants harbouring CP and MP genes supported the accumulation of higher levels of MYMV-Vig DNA. However, MYMV-Vig accumulation was inhibited in one transgenic plant harbouring the Rep-sense gene and in two plants harbouring the T-Rep gene. Northern analysis of these plants revealed a good correlation between expression of Rep or T-Rep genes and inhibition of MYMV-Vig accumulation.

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Section: Introductionmentioning

confidence: 82%

Expression of full-length and truncated Rep genes from Mungbean yellow mosaic virus-Vigna inhibits viral replication in transgenic tobacco

et al. 2006

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“…Mungbean yellow mosaic disease is transmitted by the vector, the whitefly (Bemisia tabaci). It is found to spread the begomoviruses, the major hazard to the flourishing production of mungbean in India, Sri Lanka, Pakistan, Bangladesh, Papu New Guinea, Philippines and Thailand (Honda et al 1983;Chenulu & Verma 1988;Varma et al 1992;Jones 2003;Haq et al 2011a). Based on sequence identity analyses, the bipartite begomovirus isolates, namely, mungbean yellow mosaic virus (MYMV), mungbean yellow mosaic India virus (MYMIV) and horse gram yellow mosaic virus (HgYMV) are recognized as the causal agents of MYMD in different regions of the world (Qazi et al 2007;Malathi & John 2008a;Ilyas et al 2010).…”

Section: Mungbean Yellow Mosaic Virus (Mymv): a Threat To Green Gram mentioning

confidence: 99%

Mungbean yellow mosaic virus (MYMV): a threat to green gram (Vigna radiata) production in Asia

Karthikeyan

Shobhana

Manickam

et al. 2014

International Journal of Pest Management

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Mungbean yellow mosaic virus (MYMV) disease is one of the most vicious diseases of green gram and has been renowned in India for more than five decades. It is caused by a group of geminiviruses belonging to the genus, begomovirus of the family, Geminiviridae. They are transmitted through whitefly in a persistent manner. The economic losses due to this virus account up to 85% in green gram which is spreading faster towards newer areas. The escalating economic importance of MYMV has resulted in the call for accurate detection and identification procedures that inspire rigorous research efforts focussing on the biology, diversity and epidemiology of the virus, so that viable management strategies could be designed. Breeding for resistance or tolerance appears to be the best approach to control this disease. However, the commercially offered genotypes are only partially resistant. Therefore, the hunt for newer sources of disease resistance needs to be intensified. This review updates all the accessible information on MYMV and outlines the areas in which advance research is indispensable.

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“…Almost all available varieties are vulnerable to disease and damage may approach up to 100% yield losses in severe epidemics (Sudha et al, 2013;Mohan et al, 2014). The disease is a major threat to mungbean cultivation in different countries including India, Sri-Lanka, Pakistan, Bangladesh, Papu New Guinea, Philippines and Thailand (Honda et al, 1983;Chenulu and Verma, 1988). The disease was first time reported in India on lima bean in 1940 (Capoor and Varma, 1948), in Dolichos (Capoor and Varma, 1950) and in mungbean (Nariani, 1960).…”

Section: Introductionmentioning

confidence: 99%

Exploration of Durable Resistance in Mungbean Against Mungbean Yellow Mosaic Disease

Akbar¹

2017

PAKJAS

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Mungbean yellow mosaic disease is most damaging disease of mungbean in all of its growing areas. Genetic resistance is a viable and environment friendly solution, for which resistance source is prerequisite. Total 83 mungbean genotypes were experimented for their resistance potential against disease for two consecutive years 2014 and 2015 during summer season. Infection percentage and disease severity based scoring scales were compared for their discrimination potential for screening. Disease severity based scoring scale was found superior to infection percentage based scale because it categorized genotypes into five groups while only two groups were generated by later. Infection %age ranged from 33.33 to 100% while disease severity index varied from 10 to 75%. Only one genotype (NM 6-68-2) was found comparatively tolerant (D.I. 10%) during both the years. Biplot analysis depicted that behavior of some genotypes showed instability in the second year while most of the genotypes showed a stable resistance/susceptibility levels during both the years. Accession NM 1-32-1 was highly susceptible genotype during both the years. Disease severity showed maximum correlation with chlorophyll-a reduction followed by 100 seed weight while it had high level of association with yield. Analytical dissection of correlation among traits revealed that direct effect of disease on yield reduction was negligible but it caused yield reduction via reduced harvest index and 100 seed weight. It is concluded that resistance sources are very scarce and resistance level is very low but still these sources can be exploited in breeding programs. For the improvement of genetic variability induced mutation could be another option.

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Mechanical Transmission, Purification, and Some Properties of Whitefly-Borne Mung Bean Yellow Mosaic Virus in Thailand

Cited by 50 publications

References 1 publication

Expression of full-length and truncated Rep genes from Mungbean yellow mosaic virus-Vigna inhibits viral replication in transgenic tobacco

Expression of full-length and truncated Rep genes from Mungbean yellow mosaic virus-Vigna inhibits viral replication in transgenic tobacco

Mungbean yellow mosaic virus (MYMV): a threat to green gram (Vigna radiata) production in Asia

Exploration of Durable Resistance in Mungbean Against Mungbean Yellow Mosaic Disease

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